A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein

This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated tha...

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Detalles Bibliográficos
Autores principales: Kawano, Ryuji, Tsuji, Yutaro, Kamiya, Koki, Kodama, Taiga, Osaki, Toshihisa, Miki, Norihisa, Takeuchi, Shoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114450/
https://www.ncbi.nlm.nih.gov/pubmed/25072468
http://dx.doi.org/10.1371/journal.pone.0102427
Descripción
Sumario:This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor.

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