A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein

This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated tha...

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Detalles Bibliográficos
Autores principales: Kawano, Ryuji, Tsuji, Yutaro, Kamiya, Koki, Kodama, Taiga, Osaki, Toshihisa, Miki, Norihisa, Takeuchi, Shoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114450/
https://www.ncbi.nlm.nih.gov/pubmed/25072468
http://dx.doi.org/10.1371/journal.pone.0102427
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author Kawano, Ryuji
Tsuji, Yutaro
Kamiya, Koki
Kodama, Taiga
Osaki, Toshihisa
Miki, Norihisa
Takeuchi, Shoji
author_facet Kawano, Ryuji
Tsuji, Yutaro
Kamiya, Koki
Kodama, Taiga
Osaki, Toshihisa
Miki, Norihisa
Takeuchi, Shoji
author_sort Kawano, Ryuji
collection PubMed
description This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor.
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spelling pubmed-41144502014-08-04 A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein Kawano, Ryuji Tsuji, Yutaro Kamiya, Koki Kodama, Taiga Osaki, Toshihisa Miki, Norihisa Takeuchi, Shoji PLoS One Research Article This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor. Public Library of Science 2014-07-29 /pmc/articles/PMC4114450/ /pubmed/25072468 http://dx.doi.org/10.1371/journal.pone.0102427 Text en © 2014 Kawano et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kawano, Ryuji
Tsuji, Yutaro
Kamiya, Koki
Kodama, Taiga
Osaki, Toshihisa
Miki, Norihisa
Takeuchi, Shoji
A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
title A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
title_full A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
title_fullStr A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
title_full_unstemmed A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
title_short A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
title_sort portable lipid bilayer system for environmental sensing with a transmembrane protein
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114450/
https://www.ncbi.nlm.nih.gov/pubmed/25072468
http://dx.doi.org/10.1371/journal.pone.0102427
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