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Colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase C λ/ι, E-cadherin, β-catenin and basement membrane component
Colorectal flat-type tumors include laterally spreading tumors (LSTs) and flat depressed-type tumors. The former of which shows a predominant lateral spreading growth rather than an invasive growth. The present study examined the morphological characteristics of LSTs, in comparison with polypoid- or...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114626/ https://www.ncbi.nlm.nih.gov/pubmed/25120645 http://dx.doi.org/10.3892/ol.2014.2271 |
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author | ICHIKAWA, YASUSHI NAGASHIMA, YOJI MORIOKA, KAORI AKIMOTO, KAZUNORI KOJIMA, YASUYUKI ISHIKAWA, TAKASHI GOTO, AYUMU KOBAYASHI, NORITOSHI WATANABE, KAZUTERU OTA, MITSUYOSHI FUJII, SHOICHI KAWAMATA, MAYUMI TAKAGAWA, RYO KUNIZAKI, CHIKARA TAKAHASHI, HIROKAZU NAKAJIMA, ATSUSHI MAEDA, SHIN SHIMADA, HIROSHI INAYAMA, YOSHIAKI OHNO, SHIGEO ENDO, ITARU |
author_facet | ICHIKAWA, YASUSHI NAGASHIMA, YOJI MORIOKA, KAORI AKIMOTO, KAZUNORI KOJIMA, YASUYUKI ISHIKAWA, TAKASHI GOTO, AYUMU KOBAYASHI, NORITOSHI WATANABE, KAZUTERU OTA, MITSUYOSHI FUJII, SHOICHI KAWAMATA, MAYUMI TAKAGAWA, RYO KUNIZAKI, CHIKARA TAKAHASHI, HIROKAZU NAKAJIMA, ATSUSHI MAEDA, SHIN SHIMADA, HIROSHI INAYAMA, YOSHIAKI OHNO, SHIGEO ENDO, ITARU |
author_sort | ICHIKAWA, YASUSHI |
collection | PubMed |
description | Colorectal flat-type tumors include laterally spreading tumors (LSTs) and flat depressed-type tumors. The former of which shows a predominant lateral spreading growth rather than an invasive growth. The present study examined the morphological characteristics of LSTs, in comparison with polypoid- or flat depressed-type tumors, along with the expression of atypical protein kinase C (aPKC) λ/ι, a pivotal cell polarity regulator, and the hallmarks of cell polarity, as well as with type IV collagen, β-catenin and E-cadherin. In total, 37 flat-type (24 LSTs and 13 flat depressed-type tumors) and 20 polypoid-type colorectal tumors were examined. The LSTs were classified as 15 LST adenoma (LST-A) and nine LST cancer in adenoma (LST-CA). An immunohistochemical examination was performed on aPKC λ/ι, type IV collagen, β-catenin and E-cadherin. The LST-A and -CA showed a superficial replacing growth pattern, with expression of β-catenin and E-cadherin in the basolateral membrane and type IV collagen along the basement membrane. In addition, 86.6% of LST-A and 55.6% of LST-CA showed aPKC λ/ι expression of 1+ (weak to normal intensity staining in the cytoplasm compared with the normal epithelium). Furthermore, ~45% of the polypoid-type adenomas showed 2+ (moderate intensity staining in the cytoplasm and/or nucleus) and 66.7% of the polypoid-type cancer in adenoma were 3+ (strong intensity staining in the cytoplasm and nucleus). A statistically significant positive correlation was observed between the expression of aPKC λ/ι and β-catenin (r=0.842; P<0.001), or type IV collagen (r=0.823; P<0.001). The LSTs showed a unique growth pattern, different from the expanding growth pattern presented by a polypoid tumor and invasive cancer. The growth characteristics of LST appear to be caused by adequate coexpression of β-catenin, type IV collagen and aPKC λ/ι. |
format | Online Article Text |
id | pubmed-4114626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-41146262014-08-12 Colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase C λ/ι, E-cadherin, β-catenin and basement membrane component ICHIKAWA, YASUSHI NAGASHIMA, YOJI MORIOKA, KAORI AKIMOTO, KAZUNORI KOJIMA, YASUYUKI ISHIKAWA, TAKASHI GOTO, AYUMU KOBAYASHI, NORITOSHI WATANABE, KAZUTERU OTA, MITSUYOSHI FUJII, SHOICHI KAWAMATA, MAYUMI TAKAGAWA, RYO KUNIZAKI, CHIKARA TAKAHASHI, HIROKAZU NAKAJIMA, ATSUSHI MAEDA, SHIN SHIMADA, HIROSHI INAYAMA, YOSHIAKI OHNO, SHIGEO ENDO, ITARU Oncol Lett Articles Colorectal flat-type tumors include laterally spreading tumors (LSTs) and flat depressed-type tumors. The former of which shows a predominant lateral spreading growth rather than an invasive growth. The present study examined the morphological characteristics of LSTs, in comparison with polypoid- or flat depressed-type tumors, along with the expression of atypical protein kinase C (aPKC) λ/ι, a pivotal cell polarity regulator, and the hallmarks of cell polarity, as well as with type IV collagen, β-catenin and E-cadherin. In total, 37 flat-type (24 LSTs and 13 flat depressed-type tumors) and 20 polypoid-type colorectal tumors were examined. The LSTs were classified as 15 LST adenoma (LST-A) and nine LST cancer in adenoma (LST-CA). An immunohistochemical examination was performed on aPKC λ/ι, type IV collagen, β-catenin and E-cadherin. The LST-A and -CA showed a superficial replacing growth pattern, with expression of β-catenin and E-cadherin in the basolateral membrane and type IV collagen along the basement membrane. In addition, 86.6% of LST-A and 55.6% of LST-CA showed aPKC λ/ι expression of 1+ (weak to normal intensity staining in the cytoplasm compared with the normal epithelium). Furthermore, ~45% of the polypoid-type adenomas showed 2+ (moderate intensity staining in the cytoplasm and/or nucleus) and 66.7% of the polypoid-type cancer in adenoma were 3+ (strong intensity staining in the cytoplasm and nucleus). A statistically significant positive correlation was observed between the expression of aPKC λ/ι and β-catenin (r=0.842; P<0.001), or type IV collagen (r=0.823; P<0.001). The LSTs showed a unique growth pattern, different from the expanding growth pattern presented by a polypoid tumor and invasive cancer. The growth characteristics of LST appear to be caused by adequate coexpression of β-catenin, type IV collagen and aPKC λ/ι. D.A. Spandidos 2014-09 2014-06-20 /pmc/articles/PMC4114626/ /pubmed/25120645 http://dx.doi.org/10.3892/ol.2014.2271 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles ICHIKAWA, YASUSHI NAGASHIMA, YOJI MORIOKA, KAORI AKIMOTO, KAZUNORI KOJIMA, YASUYUKI ISHIKAWA, TAKASHI GOTO, AYUMU KOBAYASHI, NORITOSHI WATANABE, KAZUTERU OTA, MITSUYOSHI FUJII, SHOICHI KAWAMATA, MAYUMI TAKAGAWA, RYO KUNIZAKI, CHIKARA TAKAHASHI, HIROKAZU NAKAJIMA, ATSUSHI MAEDA, SHIN SHIMADA, HIROSHI INAYAMA, YOSHIAKI OHNO, SHIGEO ENDO, ITARU Colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase C λ/ι, E-cadherin, β-catenin and basement membrane component |
title | Colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase C λ/ι, E-cadherin, β-catenin and basement membrane component |
title_full | Colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase C λ/ι, E-cadherin, β-catenin and basement membrane component |
title_fullStr | Colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase C λ/ι, E-cadherin, β-catenin and basement membrane component |
title_full_unstemmed | Colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase C λ/ι, E-cadherin, β-catenin and basement membrane component |
title_short | Colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase C λ/ι, E-cadherin, β-catenin and basement membrane component |
title_sort | colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase c λ/ι, e-cadherin, β-catenin and basement membrane component |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114626/ https://www.ncbi.nlm.nih.gov/pubmed/25120645 http://dx.doi.org/10.3892/ol.2014.2271 |
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