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Differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool?

BACKGROUND: Thyroid nodules are common, but only 5% of nodules are found to be malignant. In North America, the incidence of thyroid cancer is increasing. Fine needle aspirate (FNA) biopsy is the diagnostic test of choice. Unfortunately, up to 20% of FNAs are non-diagnostic. A specific molecular mar...

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Autores principales: Brace, Matthew D, Wang, Jun, Petten, Mark, Bullock, Martin J, Makki, Fawaz, Trites, Jonathan, Taylor, S Mark, Hart, Robert D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115165/
https://www.ncbi.nlm.nih.gov/pubmed/25927212
http://dx.doi.org/10.1186/s40463-014-0022-x
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author Brace, Matthew D
Wang, Jun
Petten, Mark
Bullock, Martin J
Makki, Fawaz
Trites, Jonathan
Taylor, S Mark
Hart, Robert D
author_facet Brace, Matthew D
Wang, Jun
Petten, Mark
Bullock, Martin J
Makki, Fawaz
Trites, Jonathan
Taylor, S Mark
Hart, Robert D
author_sort Brace, Matthew D
collection PubMed
description BACKGROUND: Thyroid nodules are common, but only 5% of nodules are found to be malignant. In North America, the incidence of thyroid cancer is increasing. Fine needle aspirate (FNA) biopsy is the diagnostic test of choice. Unfortunately, up to 20% of FNAs are non-diagnostic. A specific molecular marker for thyroid cancer is desirable. Evidence suggests that cell signaling through transforming growth factor beta (TGF- β) is important in the development of thyroid cancer. We sought to compare the expression of TGF- β in malignant and benign thyroid nodules. METHODS: From 2008-present, thyroid nodule tissue from thyroidectomy specimens was prospectively collected and stored at −80°C. RNA extraction and reverse transcription was performed on 47 samples (24 papillary thyroid cancer and 23 benign nodules). Quantitative PCR using SYBR green was performed to detect TGF-β-1 and −2. Resulting C(T) values were normalized against β-actin. Gene expression was calculated using the 2(-ΔC)(T) method. RESULTS: A significantly greater expression of TGF- β1 (p < 0.0001) was detected in the group of malignant thyroid nodules compared to benign nodules. There was no difference in the expression of TGF- β2 (p = 0.4735) between the two groups. CONCLUSIONS: In this study, we demonstrated that expression of TGF- β1 but not TGF- β2 is significantly increased in papillary thyroid cancer compared to benign thyroid nodules. This may serve as a potential diagnostic marker for papillary thyroid cancer.
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spelling pubmed-41151652014-08-05 Differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool? Brace, Matthew D Wang, Jun Petten, Mark Bullock, Martin J Makki, Fawaz Trites, Jonathan Taylor, S Mark Hart, Robert D J Otolaryngol Head Neck Surg Original Research Article BACKGROUND: Thyroid nodules are common, but only 5% of nodules are found to be malignant. In North America, the incidence of thyroid cancer is increasing. Fine needle aspirate (FNA) biopsy is the diagnostic test of choice. Unfortunately, up to 20% of FNAs are non-diagnostic. A specific molecular marker for thyroid cancer is desirable. Evidence suggests that cell signaling through transforming growth factor beta (TGF- β) is important in the development of thyroid cancer. We sought to compare the expression of TGF- β in malignant and benign thyroid nodules. METHODS: From 2008-present, thyroid nodule tissue from thyroidectomy specimens was prospectively collected and stored at −80°C. RNA extraction and reverse transcription was performed on 47 samples (24 papillary thyroid cancer and 23 benign nodules). Quantitative PCR using SYBR green was performed to detect TGF-β-1 and −2. Resulting C(T) values were normalized against β-actin. Gene expression was calculated using the 2(-ΔC)(T) method. RESULTS: A significantly greater expression of TGF- β1 (p < 0.0001) was detected in the group of malignant thyroid nodules compared to benign nodules. There was no difference in the expression of TGF- β2 (p = 0.4735) between the two groups. CONCLUSIONS: In this study, we demonstrated that expression of TGF- β1 but not TGF- β2 is significantly increased in papillary thyroid cancer compared to benign thyroid nodules. This may serve as a potential diagnostic marker for papillary thyroid cancer. BioMed Central 2014-07-18 /pmc/articles/PMC4115165/ /pubmed/25927212 http://dx.doi.org/10.1186/s40463-014-0022-x Text en Copyright © 2014 Brace et al. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Original Research Article
Brace, Matthew D
Wang, Jun
Petten, Mark
Bullock, Martin J
Makki, Fawaz
Trites, Jonathan
Taylor, S Mark
Hart, Robert D
Differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool?
title Differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool?
title_full Differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool?
title_fullStr Differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool?
title_full_unstemmed Differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool?
title_short Differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool?
title_sort differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool?
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115165/
https://www.ncbi.nlm.nih.gov/pubmed/25927212
http://dx.doi.org/10.1186/s40463-014-0022-x
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