High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis

The efficiency of one-step and multi-step protocols of DNA isolation from lysed sputum samples containing the Mycobacterium tuberculosis complex has been compared. DNA was isolated using spin-cartridges containing a special silica-based sorbent modified with fluoroplast and polyaniline, or using an...

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Detalles Bibliográficos
Autores principales: Kapustin, D. V., Prostyakova, A. I., Alexeev, Ya. I., Varlamov, D. A., Zubov, V. P., Zavriev, S. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115226/
https://www.ncbi.nlm.nih.gov/pubmed/25093111
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author Kapustin, D. V.
Prostyakova, A. I.
Alexeev, Ya. I.
Varlamov, D. A.
Zubov, V. P.
Zavriev, S. K.
author_facet Kapustin, D. V.
Prostyakova, A. I.
Alexeev, Ya. I.
Varlamov, D. A.
Zubov, V. P.
Zavriev, S. K.
author_sort Kapustin, D. V.
collection PubMed
description The efficiency of one-step and multi-step protocols of DNA isolation from lysed sputum samples containing the Mycobacterium tuberculosis complex has been compared. DNA was isolated using spin-cartridges containing a special silica-based sorbent modified with fluoroplast and polyaniline, or using an automated isolation system. One-step isolation using the obtained sorbent has been shown to ensure a significantly lower DNA loss and higher sensitivity in the PCR detection of Mycobacterium tuberculosis as compared to a system based on sorption and desorption of nucleic acids during the isolation.
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spelling pubmed-41152262014-08-04 High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis Kapustin, D. V. Prostyakova, A. I. Alexeev, Ya. I. Varlamov, D. A. Zubov, V. P. Zavriev, S. K. Acta Naturae Research Article The efficiency of one-step and multi-step protocols of DNA isolation from lysed sputum samples containing the Mycobacterium tuberculosis complex has been compared. DNA was isolated using spin-cartridges containing a special silica-based sorbent modified with fluoroplast and polyaniline, or using an automated isolation system. One-step isolation using the obtained sorbent has been shown to ensure a significantly lower DNA loss and higher sensitivity in the PCR detection of Mycobacterium tuberculosis as compared to a system based on sorption and desorption of nucleic acids during the isolation. A.I. Gordeyev 2014 /pmc/articles/PMC4115226/ /pubmed/25093111 Text en Copyright ® 2014 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kapustin, D. V.
Prostyakova, A. I.
Alexeev, Ya. I.
Varlamov, D. A.
Zubov, V. P.
Zavriev, S. K.
High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis
title High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis
title_full High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis
title_fullStr High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis
title_full_unstemmed High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis
title_short High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis
title_sort high-throughput method of one-step dna isolation for pcr diagnostics of mycobacterium tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115226/
https://www.ncbi.nlm.nih.gov/pubmed/25093111
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