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Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds

The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involv...

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Autores principales: Ribeiro, Jorge, Pereira, Tiago, Amorim, Irina, Caseiro, Ana Rita, Lopes, Maria A, Lima, Joana, Gartner, Andrea, Santos, José Domingos, Bártolo, Paulo J, Rodrigues, Jorge Manuel, Mauricio, Ana Colette, Luís, Ana Lúcia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115236/
https://www.ncbi.nlm.nih.gov/pubmed/25076843
http://dx.doi.org/10.7150/ijms.9139
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author Ribeiro, Jorge
Pereira, Tiago
Amorim, Irina
Caseiro, Ana Rita
Lopes, Maria A
Lima, Joana
Gartner, Andrea
Santos, José Domingos
Bártolo, Paulo J
Rodrigues, Jorge Manuel
Mauricio, Ana Colette
Luís, Ana Lúcia
author_facet Ribeiro, Jorge
Pereira, Tiago
Amorim, Irina
Caseiro, Ana Rita
Lopes, Maria A
Lima, Joana
Gartner, Andrea
Santos, José Domingos
Bártolo, Paulo J
Rodrigues, Jorge Manuel
Mauricio, Ana Colette
Luís, Ana Lúcia
author_sort Ribeiro, Jorge
collection PubMed
description The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem cells (MSCs) are nowadays a promising treatment of skin ulcers which are a cause of high morbidity. The MSCs modulate the inflammatory local response and induce cell replacing, by a paracrine mode of action, being an important cell therapy for the impaired wound healing. The local application of human MSCs (hMSCs) isolated from the umbilical cord Wharton's jelly together with a poly(vinyl alcohol) hydrogel (PVA) membrane, was tested to promote wound healing in two dogs that were referred for clinical examination at UPVET Hospital, showing non-healing large skin lesions by the standard treatments. The wounds were infiltrated with 1000 cells/µl hMSCs in a total volume of 100 µl per cm(2) of lesion area. A PVA membrane was applied to completely cover the wound to prevent its dehydration. Both animals after the treatment demonstrated a significant progress in skin regeneration with decreased extent of ulcerated areas confirmed by histological analysis. The use of Wharton's jelly MSCs associated with a PVA membrane showed promising clinical results for future application in the treatment of chronic wounds in companion animals and humans.
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spelling pubmed-41152362014-07-30 Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds Ribeiro, Jorge Pereira, Tiago Amorim, Irina Caseiro, Ana Rita Lopes, Maria A Lima, Joana Gartner, Andrea Santos, José Domingos Bártolo, Paulo J Rodrigues, Jorge Manuel Mauricio, Ana Colette Luís, Ana Lúcia Int J Med Sci Research Paper The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem cells (MSCs) are nowadays a promising treatment of skin ulcers which are a cause of high morbidity. The MSCs modulate the inflammatory local response and induce cell replacing, by a paracrine mode of action, being an important cell therapy for the impaired wound healing. The local application of human MSCs (hMSCs) isolated from the umbilical cord Wharton's jelly together with a poly(vinyl alcohol) hydrogel (PVA) membrane, was tested to promote wound healing in two dogs that were referred for clinical examination at UPVET Hospital, showing non-healing large skin lesions by the standard treatments. The wounds were infiltrated with 1000 cells/µl hMSCs in a total volume of 100 µl per cm(2) of lesion area. A PVA membrane was applied to completely cover the wound to prevent its dehydration. Both animals after the treatment demonstrated a significant progress in skin regeneration with decreased extent of ulcerated areas confirmed by histological analysis. The use of Wharton's jelly MSCs associated with a PVA membrane showed promising clinical results for future application in the treatment of chronic wounds in companion animals and humans. Ivyspring International Publisher 2014-07-11 /pmc/articles/PMC4115236/ /pubmed/25076843 http://dx.doi.org/10.7150/ijms.9139 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Ribeiro, Jorge
Pereira, Tiago
Amorim, Irina
Caseiro, Ana Rita
Lopes, Maria A
Lima, Joana
Gartner, Andrea
Santos, José Domingos
Bártolo, Paulo J
Rodrigues, Jorge Manuel
Mauricio, Ana Colette
Luís, Ana Lúcia
Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds
title Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds
title_full Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds
title_fullStr Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds
title_full_unstemmed Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds
title_short Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds
title_sort cell therapy with human mscs isolated from the umbilical cord wharton jelly associated to a pva membrane in the treatment of chronic skin wounds
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115236/
https://www.ncbi.nlm.nih.gov/pubmed/25076843
http://dx.doi.org/10.7150/ijms.9139
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