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Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds
The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involv...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115236/ https://www.ncbi.nlm.nih.gov/pubmed/25076843 http://dx.doi.org/10.7150/ijms.9139 |
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author | Ribeiro, Jorge Pereira, Tiago Amorim, Irina Caseiro, Ana Rita Lopes, Maria A Lima, Joana Gartner, Andrea Santos, José Domingos Bártolo, Paulo J Rodrigues, Jorge Manuel Mauricio, Ana Colette Luís, Ana Lúcia |
author_facet | Ribeiro, Jorge Pereira, Tiago Amorim, Irina Caseiro, Ana Rita Lopes, Maria A Lima, Joana Gartner, Andrea Santos, José Domingos Bártolo, Paulo J Rodrigues, Jorge Manuel Mauricio, Ana Colette Luís, Ana Lúcia |
author_sort | Ribeiro, Jorge |
collection | PubMed |
description | The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem cells (MSCs) are nowadays a promising treatment of skin ulcers which are a cause of high morbidity. The MSCs modulate the inflammatory local response and induce cell replacing, by a paracrine mode of action, being an important cell therapy for the impaired wound healing. The local application of human MSCs (hMSCs) isolated from the umbilical cord Wharton's jelly together with a poly(vinyl alcohol) hydrogel (PVA) membrane, was tested to promote wound healing in two dogs that were referred for clinical examination at UPVET Hospital, showing non-healing large skin lesions by the standard treatments. The wounds were infiltrated with 1000 cells/µl hMSCs in a total volume of 100 µl per cm(2) of lesion area. A PVA membrane was applied to completely cover the wound to prevent its dehydration. Both animals after the treatment demonstrated a significant progress in skin regeneration with decreased extent of ulcerated areas confirmed by histological analysis. The use of Wharton's jelly MSCs associated with a PVA membrane showed promising clinical results for future application in the treatment of chronic wounds in companion animals and humans. |
format | Online Article Text |
id | pubmed-4115236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-41152362014-07-30 Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds Ribeiro, Jorge Pereira, Tiago Amorim, Irina Caseiro, Ana Rita Lopes, Maria A Lima, Joana Gartner, Andrea Santos, José Domingos Bártolo, Paulo J Rodrigues, Jorge Manuel Mauricio, Ana Colette Luís, Ana Lúcia Int J Med Sci Research Paper The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem cells (MSCs) are nowadays a promising treatment of skin ulcers which are a cause of high morbidity. The MSCs modulate the inflammatory local response and induce cell replacing, by a paracrine mode of action, being an important cell therapy for the impaired wound healing. The local application of human MSCs (hMSCs) isolated from the umbilical cord Wharton's jelly together with a poly(vinyl alcohol) hydrogel (PVA) membrane, was tested to promote wound healing in two dogs that were referred for clinical examination at UPVET Hospital, showing non-healing large skin lesions by the standard treatments. The wounds were infiltrated with 1000 cells/µl hMSCs in a total volume of 100 µl per cm(2) of lesion area. A PVA membrane was applied to completely cover the wound to prevent its dehydration. Both animals after the treatment demonstrated a significant progress in skin regeneration with decreased extent of ulcerated areas confirmed by histological analysis. The use of Wharton's jelly MSCs associated with a PVA membrane showed promising clinical results for future application in the treatment of chronic wounds in companion animals and humans. Ivyspring International Publisher 2014-07-11 /pmc/articles/PMC4115236/ /pubmed/25076843 http://dx.doi.org/10.7150/ijms.9139 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Ribeiro, Jorge Pereira, Tiago Amorim, Irina Caseiro, Ana Rita Lopes, Maria A Lima, Joana Gartner, Andrea Santos, José Domingos Bártolo, Paulo J Rodrigues, Jorge Manuel Mauricio, Ana Colette Luís, Ana Lúcia Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds |
title | Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds |
title_full | Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds |
title_fullStr | Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds |
title_full_unstemmed | Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds |
title_short | Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds |
title_sort | cell therapy with human mscs isolated from the umbilical cord wharton jelly associated to a pva membrane in the treatment of chronic skin wounds |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115236/ https://www.ncbi.nlm.nih.gov/pubmed/25076843 http://dx.doi.org/10.7150/ijms.9139 |
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