Cargando…

Molecular Phylogenetic Analysis Reveals the New Genus Hemisphaericaspora of the Family Debaryomycetaceae

Four strains of a novel ascomycetous yeast species were recovered from the frass of wood-boring beetles collected from the Baotianman Nature Reserve and the Laojieling Nature Reserve in Henan Province, China. This species produced unconjugated and deliquescent asci with hemispheroid or helmet-shaped...

Descripción completa

Detalles Bibliográficos
Autores principales: Hui, Fengli, Ren, Yongcheng, Chen, Liang, Li, Ying, Zhang, Lin, Niu, Qiuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116224/
https://www.ncbi.nlm.nih.gov/pubmed/25075963
http://dx.doi.org/10.1371/journal.pone.0103737
_version_ 1782328586341974016
author Hui, Fengli
Ren, Yongcheng
Chen, Liang
Li, Ying
Zhang, Lin
Niu, Qiuhong
author_facet Hui, Fengli
Ren, Yongcheng
Chen, Liang
Li, Ying
Zhang, Lin
Niu, Qiuhong
author_sort Hui, Fengli
collection PubMed
description Four strains of a novel ascomycetous yeast species were recovered from the frass of wood-boring beetles collected from the Baotianman Nature Reserve and the Laojieling Nature Reserve in Henan Province, China. This species produced unconjugated and deliquescent asci with hemispheroid or helmet-shaped ascospores. Analysis of gene sequences for the D1/D2 domain of the large subunit (LSU) rRNA, as well as analysis of concatenated gene sequences for the nearly complete small subunit (SSU) rRNA and D1/D2 domain of the large subunit (LSU) rRNA placed the novel species in a small clade including only one recognised species, Candida insectamans, in the family Debaryomycetaceae (Saccharomycotina, Ascomycota). DNA sequence analyses demonstrated that the novel species was distinct from all currently recognised teleomorphic yeast genus. The name Hemisphaericaspora nanyangensis gen nov., sp. nov. is proposed to accommodate the novel genus and species. The new genus can be distinguished from closely related teleomorphic genera Lodderomyces and Spathaspora through sequence comparison and ascospore morphology. The ex-type strain of H. nanyangensis is CBS 13020(T) ( = CICC 33021 = NYNU 13717). Furthermore, based on phenotypic and genotypic characteristics, C. insectamans is transferred to the newly described genus as Hemisphaericaspora insectamans comb. nov., in accordance with the changes in the International Code of Nomenclature for algae, fungi and plants.
format Online
Article
Text
id pubmed-4116224
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41162242014-08-04 Molecular Phylogenetic Analysis Reveals the New Genus Hemisphaericaspora of the Family Debaryomycetaceae Hui, Fengli Ren, Yongcheng Chen, Liang Li, Ying Zhang, Lin Niu, Qiuhong PLoS One Research Article Four strains of a novel ascomycetous yeast species were recovered from the frass of wood-boring beetles collected from the Baotianman Nature Reserve and the Laojieling Nature Reserve in Henan Province, China. This species produced unconjugated and deliquescent asci with hemispheroid or helmet-shaped ascospores. Analysis of gene sequences for the D1/D2 domain of the large subunit (LSU) rRNA, as well as analysis of concatenated gene sequences for the nearly complete small subunit (SSU) rRNA and D1/D2 domain of the large subunit (LSU) rRNA placed the novel species in a small clade including only one recognised species, Candida insectamans, in the family Debaryomycetaceae (Saccharomycotina, Ascomycota). DNA sequence analyses demonstrated that the novel species was distinct from all currently recognised teleomorphic yeast genus. The name Hemisphaericaspora nanyangensis gen nov., sp. nov. is proposed to accommodate the novel genus and species. The new genus can be distinguished from closely related teleomorphic genera Lodderomyces and Spathaspora through sequence comparison and ascospore morphology. The ex-type strain of H. nanyangensis is CBS 13020(T) ( = CICC 33021 = NYNU 13717). Furthermore, based on phenotypic and genotypic characteristics, C. insectamans is transferred to the newly described genus as Hemisphaericaspora insectamans comb. nov., in accordance with the changes in the International Code of Nomenclature for algae, fungi and plants. Public Library of Science 2014-07-30 /pmc/articles/PMC4116224/ /pubmed/25075963 http://dx.doi.org/10.1371/journal.pone.0103737 Text en © 2014 Hui et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hui, Fengli
Ren, Yongcheng
Chen, Liang
Li, Ying
Zhang, Lin
Niu, Qiuhong
Molecular Phylogenetic Analysis Reveals the New Genus Hemisphaericaspora of the Family Debaryomycetaceae
title Molecular Phylogenetic Analysis Reveals the New Genus Hemisphaericaspora of the Family Debaryomycetaceae
title_full Molecular Phylogenetic Analysis Reveals the New Genus Hemisphaericaspora of the Family Debaryomycetaceae
title_fullStr Molecular Phylogenetic Analysis Reveals the New Genus Hemisphaericaspora of the Family Debaryomycetaceae
title_full_unstemmed Molecular Phylogenetic Analysis Reveals the New Genus Hemisphaericaspora of the Family Debaryomycetaceae
title_short Molecular Phylogenetic Analysis Reveals the New Genus Hemisphaericaspora of the Family Debaryomycetaceae
title_sort molecular phylogenetic analysis reveals the new genus hemisphaericaspora of the family debaryomycetaceae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116224/
https://www.ncbi.nlm.nih.gov/pubmed/25075963
http://dx.doi.org/10.1371/journal.pone.0103737
work_keys_str_mv AT huifengli molecularphylogeneticanalysisrevealsthenewgenushemisphaericasporaofthefamilydebaryomycetaceae
AT renyongcheng molecularphylogeneticanalysisrevealsthenewgenushemisphaericasporaofthefamilydebaryomycetaceae
AT chenliang molecularphylogeneticanalysisrevealsthenewgenushemisphaericasporaofthefamilydebaryomycetaceae
AT liying molecularphylogeneticanalysisrevealsthenewgenushemisphaericasporaofthefamilydebaryomycetaceae
AT zhanglin molecularphylogeneticanalysisrevealsthenewgenushemisphaericasporaofthefamilydebaryomycetaceae
AT niuqiuhong molecularphylogeneticanalysisrevealsthenewgenushemisphaericasporaofthefamilydebaryomycetaceae