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Ferric chloride-induced murine carotid arterial injury: A model of redox pathology()

Ferric chloride (FeCl(3)) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and...

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Detalles Bibliográficos
Autores principales: Li, Wei, McIntyre, Thomas M., Silverstein, Roy L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116643/
https://www.ncbi.nlm.nih.gov/pubmed/25101237
http://dx.doi.org/10.1016/j.redox.2012.11.001
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author Li, Wei
McIntyre, Thomas M.
Silverstein, Roy L.
author_facet Li, Wei
McIntyre, Thomas M.
Silverstein, Roy L.
author_sort Li, Wei
collection PubMed
description Ferric chloride (FeCl(3)) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and anti-platelets drugs. The time required for platelet aggregation to occlude blood flow gives a quantitative measure of vascular damage that is pathologically relevant to thrombotic disease. We have refined the traditional FeCl(3)-induced carotid artery model making the data highly reproducible with lower variation. This paper will describe our artifices and report the role of varying the oxidative damage by varying FeCl(3) concentrations and exposure. To explore a maximum difference between experimental groups, adjustment of the selected FeCl(3) dose and exposure duration may be necessary.
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spelling pubmed-41166432014-08-06 Ferric chloride-induced murine carotid arterial injury: A model of redox pathology() Li, Wei McIntyre, Thomas M. Silverstein, Roy L. Redox Biol Method Ferric chloride (FeCl(3)) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and anti-platelets drugs. The time required for platelet aggregation to occlude blood flow gives a quantitative measure of vascular damage that is pathologically relevant to thrombotic disease. We have refined the traditional FeCl(3)-induced carotid artery model making the data highly reproducible with lower variation. This paper will describe our artifices and report the role of varying the oxidative damage by varying FeCl(3) concentrations and exposure. To explore a maximum difference between experimental groups, adjustment of the selected FeCl(3) dose and exposure duration may be necessary. Elsevier 2013-01-26 /pmc/articles/PMC4116643/ /pubmed/25101237 http://dx.doi.org/10.1016/j.redox.2012.11.001 Text en © 2013 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Method
Li, Wei
McIntyre, Thomas M.
Silverstein, Roy L.
Ferric chloride-induced murine carotid arterial injury: A model of redox pathology()
title Ferric chloride-induced murine carotid arterial injury: A model of redox pathology()
title_full Ferric chloride-induced murine carotid arterial injury: A model of redox pathology()
title_fullStr Ferric chloride-induced murine carotid arterial injury: A model of redox pathology()
title_full_unstemmed Ferric chloride-induced murine carotid arterial injury: A model of redox pathology()
title_short Ferric chloride-induced murine carotid arterial injury: A model of redox pathology()
title_sort ferric chloride-induced murine carotid arterial injury: a model of redox pathology()
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116643/
https://www.ncbi.nlm.nih.gov/pubmed/25101237
http://dx.doi.org/10.1016/j.redox.2012.11.001
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