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Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria
Under stress conditions, many species of bacteria enter into starvation mode of metabolism or a physiologically viable but non-culturable (VBNC) state. Several human pathogenic bacteria have been reported to enter into the VBNC state under these conditions. The pathogenic VBNC bacteria cannot be gro...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116801/ https://www.ncbi.nlm.nih.gov/pubmed/25133139 http://dx.doi.org/10.3389/fpubh.2014.00103 |
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author | Ramamurthy, Thandavarayan Ghosh, Amit Pazhani, Gururaja P. Shinoda, Sumio |
author_facet | Ramamurthy, Thandavarayan Ghosh, Amit Pazhani, Gururaja P. Shinoda, Sumio |
author_sort | Ramamurthy, Thandavarayan |
collection | PubMed |
description | Under stress conditions, many species of bacteria enter into starvation mode of metabolism or a physiologically viable but non-culturable (VBNC) state. Several human pathogenic bacteria have been reported to enter into the VBNC state under these conditions. The pathogenic VBNC bacteria cannot be grown using conventional culture media, although they continue to retain their viability and express their virulence. Though there have been debates on the VBNC concept in the past, several molecular studies have shown that not only can the VBNC state be induced under in vitro conditions but also that resuscitation from this state is possible under appropriate conditions. The most notable advance in resuscitating VBNC bacteria is the discovery of resuscitation-promoting factor (Rpf), which is a bacterial cytokines found in both Gram-positive and Gram-negative organisms. VBNC state is a survival strategy adopted by the bacteria, which has important implication in several fields, including environmental monitoring, food technology, and infectious disease management; and hence it is important to investigate the association of bacterial pathogens under VBNC state and the water/foodborne outbreaks. In this review, we describe various aspects of VBNC bacteria, which include their proteomic and genetic profiles under the VBNC state, conditions of resuscitation, methods of detection, antibiotic resistance, and observations on Rpf. |
format | Online Article Text |
id | pubmed-4116801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-41168012014-08-15 Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria Ramamurthy, Thandavarayan Ghosh, Amit Pazhani, Gururaja P. Shinoda, Sumio Front Public Health Public Health Under stress conditions, many species of bacteria enter into starvation mode of metabolism or a physiologically viable but non-culturable (VBNC) state. Several human pathogenic bacteria have been reported to enter into the VBNC state under these conditions. The pathogenic VBNC bacteria cannot be grown using conventional culture media, although they continue to retain their viability and express their virulence. Though there have been debates on the VBNC concept in the past, several molecular studies have shown that not only can the VBNC state be induced under in vitro conditions but also that resuscitation from this state is possible under appropriate conditions. The most notable advance in resuscitating VBNC bacteria is the discovery of resuscitation-promoting factor (Rpf), which is a bacterial cytokines found in both Gram-positive and Gram-negative organisms. VBNC state is a survival strategy adopted by the bacteria, which has important implication in several fields, including environmental monitoring, food technology, and infectious disease management; and hence it is important to investigate the association of bacterial pathogens under VBNC state and the water/foodborne outbreaks. In this review, we describe various aspects of VBNC bacteria, which include their proteomic and genetic profiles under the VBNC state, conditions of resuscitation, methods of detection, antibiotic resistance, and observations on Rpf. Frontiers Media S.A. 2014-07-31 /pmc/articles/PMC4116801/ /pubmed/25133139 http://dx.doi.org/10.3389/fpubh.2014.00103 Text en Copyright © 2014 Ramamurthy, Ghosh, Pazhani and Shinoda. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Public Health Ramamurthy, Thandavarayan Ghosh, Amit Pazhani, Gururaja P. Shinoda, Sumio Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria |
title | Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria |
title_full | Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria |
title_fullStr | Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria |
title_full_unstemmed | Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria |
title_short | Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria |
title_sort | current perspectives on viable but non-culturable (vbnc) pathogenic bacteria |
topic | Public Health |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116801/ https://www.ncbi.nlm.nih.gov/pubmed/25133139 http://dx.doi.org/10.3389/fpubh.2014.00103 |
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