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Polarizing intestinal epithelial cells electrically through Ror2
The apicobasal polarity of enterocytes determines where the brush border membrane (apical membrane) will form, but how this apical membrane faces the lumen is not well understood. The electrical signal across the epithelium could serve as a coordinating cue, orienting and polarizing enterocytes. Her...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117229/ https://www.ncbi.nlm.nih.gov/pubmed/24928904 http://dx.doi.org/10.1242/jcs.146357 |
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author | Cao, Lin McCaig, Colin D. Scott, Roderick H. Zhao, Siwei Milne, Gillian Clevers, Hans Zhao, Min Pu, Jin |
author_facet | Cao, Lin McCaig, Colin D. Scott, Roderick H. Zhao, Siwei Milne, Gillian Clevers, Hans Zhao, Min Pu, Jin |
author_sort | Cao, Lin |
collection | PubMed |
description | The apicobasal polarity of enterocytes determines where the brush border membrane (apical membrane) will form, but how this apical membrane faces the lumen is not well understood. The electrical signal across the epithelium could serve as a coordinating cue, orienting and polarizing enterocytes. Here, we show that applying a physiological electric field to intestinal epithelial cells, to mimic the natural electric field created by the transepithelial potential difference, polarized phosphorylation of the actin-binding protein ezrin, increased expression of intestinal alkaline phosphatase (ALPI, a differentiation marker) and remodeled the actin cytoskeleton selectively on the cathode side. In addition, an applied electric field also activated ERK1/2 and LKB1 (also known as STK11), key molecules in apical membrane formation. Disruption of the tyrosine protein kinase transmembrane receptor Ror2 suppressed activation of ERK1/2 and LKB1 significantly, and subsequently inhibited apical membrane formation in enterocytes. Our findings indicate that the endogenous electric field created by the transepithelial potential difference might act as an essential coordinating signal for apical membrane formation at a tissue level, through activation of LKB1 mediated by Ror2–ERK signaling. |
format | Online Article Text |
id | pubmed-4117229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-41172292014-08-19 Polarizing intestinal epithelial cells electrically through Ror2 Cao, Lin McCaig, Colin D. Scott, Roderick H. Zhao, Siwei Milne, Gillian Clevers, Hans Zhao, Min Pu, Jin J Cell Sci Short Report The apicobasal polarity of enterocytes determines where the brush border membrane (apical membrane) will form, but how this apical membrane faces the lumen is not well understood. The electrical signal across the epithelium could serve as a coordinating cue, orienting and polarizing enterocytes. Here, we show that applying a physiological electric field to intestinal epithelial cells, to mimic the natural electric field created by the transepithelial potential difference, polarized phosphorylation of the actin-binding protein ezrin, increased expression of intestinal alkaline phosphatase (ALPI, a differentiation marker) and remodeled the actin cytoskeleton selectively on the cathode side. In addition, an applied electric field also activated ERK1/2 and LKB1 (also known as STK11), key molecules in apical membrane formation. Disruption of the tyrosine protein kinase transmembrane receptor Ror2 suppressed activation of ERK1/2 and LKB1 significantly, and subsequently inhibited apical membrane formation in enterocytes. Our findings indicate that the endogenous electric field created by the transepithelial potential difference might act as an essential coordinating signal for apical membrane formation at a tissue level, through activation of LKB1 mediated by Ror2–ERK signaling. The Company of Biologists 2014-08-01 /pmc/articles/PMC4117229/ /pubmed/24928904 http://dx.doi.org/10.1242/jcs.146357 Text en © 2014. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Short Report Cao, Lin McCaig, Colin D. Scott, Roderick H. Zhao, Siwei Milne, Gillian Clevers, Hans Zhao, Min Pu, Jin Polarizing intestinal epithelial cells electrically through Ror2 |
title | Polarizing intestinal epithelial cells electrically through Ror2 |
title_full | Polarizing intestinal epithelial cells electrically through Ror2 |
title_fullStr | Polarizing intestinal epithelial cells electrically through Ror2 |
title_full_unstemmed | Polarizing intestinal epithelial cells electrically through Ror2 |
title_short | Polarizing intestinal epithelial cells electrically through Ror2 |
title_sort | polarizing intestinal epithelial cells electrically through ror2 |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117229/ https://www.ncbi.nlm.nih.gov/pubmed/24928904 http://dx.doi.org/10.1242/jcs.146357 |
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