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Co-Expression and Co-Purification of Archaeal and Eukaryal Box C/D RNPs

Box C/D ribonucleoprotein particles (RNPs) are 2′-O-methylation enzymes required for maturation of ribosomal and small nuclear RNA. Previous biochemical and structural studies of the box C/D RNPs were limited by the unavailability of purified intact RNPs. We developed a bacterial co-expression strat...

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Detalles Bibliográficos
Autores principales: Peng, Yu, Yu, Ge, Tian, Shaoxiong, Li, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117494/
https://www.ncbi.nlm.nih.gov/pubmed/25078083
http://dx.doi.org/10.1371/journal.pone.0103096

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