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Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model

Experimental neural cell therapies, including donor neural stem/progenitor cells (NPCs) have been reported to offer beneficial effects on the recovery after an injury and to counteract inflammatory and degenerative processes in the central nervous system (CNS). The interplay between donor neural cel...

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Autores principales: Liu, Jia, Hjorth, Erik, Zhu, Mingqin, Calzarossa, Cinzia, Samuelsson, Eva-Britt, Schultzberg, Marianne, Åkesson, Elisabet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117556/
https://www.ncbi.nlm.nih.gov/pubmed/24034597
http://dx.doi.org/10.1111/jcmm.12123
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author Liu, Jia
Hjorth, Erik
Zhu, Mingqin
Calzarossa, Cinzia
Samuelsson, Eva-Britt
Schultzberg, Marianne
Åkesson, Elisabet
author_facet Liu, Jia
Hjorth, Erik
Zhu, Mingqin
Calzarossa, Cinzia
Samuelsson, Eva-Britt
Schultzberg, Marianne
Åkesson, Elisabet
author_sort Liu, Jia
collection PubMed
description Experimental neural cell therapies, including donor neural stem/progenitor cells (NPCs) have been reported to offer beneficial effects on the recovery after an injury and to counteract inflammatory and degenerative processes in the central nervous system (CNS). The interplay between donor neural cells and the host CNS still to a large degree remains unclear, in particular in human allogeneic conditions. Here, we focused our studies on the interaction of human NPCs and microglia utilizing a co-culture model. In co-cultures, both NPCs and microglia showed increased survival and proliferation compared with mono-cultures. In the presence of microglia, a larger subpopulation of NPCs expressed the progenitor cell marker nestin, whereas a smaller group of NPCs expressed the neural markers polysialylated neural cell adhesion molecule, A2B5 and glial fibrillary acidic protein compared with NPC mono-cultures. Microglia thus hindered differentiation of NPCs. The presence of human NPCs increased microglial phagocytosis of latex beads. Furthermore, we observed that the expression of CD200 molecules on NPCs and the CD200 receptor protein on microglia was enhanced in co-cultures, whereas the release of transforming growth factor-β was increased suggesting anti-inflammatory features of the co-cultures. To conclude, the interplay between human allogeneic NPCs and microglia, significantly affected their respective proliferation and phenotype. Neural cell therapy including human donor NPCs may in addition to offering cell replacement, modulate host microglial phenotypes and functions to benefit neuroprotection and repair.
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spelling pubmed-41175562014-12-03 Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model Liu, Jia Hjorth, Erik Zhu, Mingqin Calzarossa, Cinzia Samuelsson, Eva-Britt Schultzberg, Marianne Åkesson, Elisabet J Cell Mol Med Original Articles Experimental neural cell therapies, including donor neural stem/progenitor cells (NPCs) have been reported to offer beneficial effects on the recovery after an injury and to counteract inflammatory and degenerative processes in the central nervous system (CNS). The interplay between donor neural cells and the host CNS still to a large degree remains unclear, in particular in human allogeneic conditions. Here, we focused our studies on the interaction of human NPCs and microglia utilizing a co-culture model. In co-cultures, both NPCs and microglia showed increased survival and proliferation compared with mono-cultures. In the presence of microglia, a larger subpopulation of NPCs expressed the progenitor cell marker nestin, whereas a smaller group of NPCs expressed the neural markers polysialylated neural cell adhesion molecule, A2B5 and glial fibrillary acidic protein compared with NPC mono-cultures. Microglia thus hindered differentiation of NPCs. The presence of human NPCs increased microglial phagocytosis of latex beads. Furthermore, we observed that the expression of CD200 molecules on NPCs and the CD200 receptor protein on microglia was enhanced in co-cultures, whereas the release of transforming growth factor-β was increased suggesting anti-inflammatory features of the co-cultures. To conclude, the interplay between human allogeneic NPCs and microglia, significantly affected their respective proliferation and phenotype. Neural cell therapy including human donor NPCs may in addition to offering cell replacement, modulate host microglial phenotypes and functions to benefit neuroprotection and repair. John Wiley & Sons 2013-11 2013-09-12 /pmc/articles/PMC4117556/ /pubmed/24034597 http://dx.doi.org/10.1111/jcmm.12123 Text en Copyright © 2013 John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Liu, Jia
Hjorth, Erik
Zhu, Mingqin
Calzarossa, Cinzia
Samuelsson, Eva-Britt
Schultzberg, Marianne
Åkesson, Elisabet
Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model
title Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model
title_full Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model
title_fullStr Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model
title_full_unstemmed Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model
title_short Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model
title_sort interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117556/
https://www.ncbi.nlm.nih.gov/pubmed/24034597
http://dx.doi.org/10.1111/jcmm.12123
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