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Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution

The telomere repeat amplification protocol (TRAP) for the human reverse transcriptase, telomerase, is a PCR-based assay developed two decades ago and is still used for routine determination of telomerase activity. The TRAP assay can only reproducibly detect ∼2-fold differences and is only quantitati...

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Detalles Bibliográficos
Autores principales:	Ludlow, Andrew T., Robin, Jerome D., Sayed, Mohammed, Litterst, Claudia M., Shelton, Dawne N., Shay, Jerry W., Wright, Woodring E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117742/ https://www.ncbi.nlm.nih.gov/pubmed/24861623 http://dx.doi.org/10.1093/nar/gku439

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