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The organization of RNA contacts by PTB for regulation of FAS splicing

Post-transcriptional steps of gene expression are regulated by RNA binding proteins. Major progress has been made in characterizing RNA-protein interactions, from high resolution structures to transcriptome-wide profiling. Due to the inherent technical challenges, less attention has been paid to the...

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Autores principales: Mickleburgh, Ian, Kafasla, Panagiota, Cherny, Dmitry, Llorian, Miriam, Curry, Stephen, Jackson, Richard J., Smith, Christopher W.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117754/
https://www.ncbi.nlm.nih.gov/pubmed/24957602
http://dx.doi.org/10.1093/nar/gku519
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author Mickleburgh, Ian
Kafasla, Panagiota
Cherny, Dmitry
Llorian, Miriam
Curry, Stephen
Jackson, Richard J.
Smith, Christopher W.J.
author_facet Mickleburgh, Ian
Kafasla, Panagiota
Cherny, Dmitry
Llorian, Miriam
Curry, Stephen
Jackson, Richard J.
Smith, Christopher W.J.
author_sort Mickleburgh, Ian
collection PubMed
description Post-transcriptional steps of gene expression are regulated by RNA binding proteins. Major progress has been made in characterizing RNA-protein interactions, from high resolution structures to transcriptome-wide profiling. Due to the inherent technical challenges, less attention has been paid to the way in which proteins with multiple RNA binding domains engage with target RNAs. We have investigated how the four RNA recognition motif (RRM) domains of Polypyrimidine tract binding (PTB) protein, a major splicing regulator, interact with FAS pre-mRNA under conditions in which PTB represses FAS exon 6 splicing. A combination of tethered hydroxyl radical probing, targeted inactivation of individual RRMs and single molecule analyses revealed an unequal division of labour between the four RRMs of PTB. RNA binding by RRM4 is the most important for function despite the low intrinsic binding specificity and the complete lack of effect of disrupting individual RRM4 contact points on the RNA. The ordered RRM3-4 di-domain packing provides an extended binding surface for RNA interacting at RRM4, via basic residues in the preceding linker. Our results illustrate how multiple alternative low-specificity binding configurations of RRM4 are consistent with repressor function as long as the overall ribonucleoprotein architecture provided by appropriate di-domain packing is maintained.
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spelling pubmed-41177542014-08-15 The organization of RNA contacts by PTB for regulation of FAS splicing Mickleburgh, Ian Kafasla, Panagiota Cherny, Dmitry Llorian, Miriam Curry, Stephen Jackson, Richard J. Smith, Christopher W.J. Nucleic Acids Res RNA Post-transcriptional steps of gene expression are regulated by RNA binding proteins. Major progress has been made in characterizing RNA-protein interactions, from high resolution structures to transcriptome-wide profiling. Due to the inherent technical challenges, less attention has been paid to the way in which proteins with multiple RNA binding domains engage with target RNAs. We have investigated how the four RNA recognition motif (RRM) domains of Polypyrimidine tract binding (PTB) protein, a major splicing regulator, interact with FAS pre-mRNA under conditions in which PTB represses FAS exon 6 splicing. A combination of tethered hydroxyl radical probing, targeted inactivation of individual RRMs and single molecule analyses revealed an unequal division of labour between the four RRMs of PTB. RNA binding by RRM4 is the most important for function despite the low intrinsic binding specificity and the complete lack of effect of disrupting individual RRM4 contact points on the RNA. The ordered RRM3-4 di-domain packing provides an extended binding surface for RNA interacting at RRM4, via basic residues in the preceding linker. Our results illustrate how multiple alternative low-specificity binding configurations of RRM4 are consistent with repressor function as long as the overall ribonucleoprotein architecture provided by appropriate di-domain packing is maintained. Oxford University Press 2014-09-01 2014-06-21 /pmc/articles/PMC4117754/ /pubmed/24957602 http://dx.doi.org/10.1093/nar/gku519 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Mickleburgh, Ian
Kafasla, Panagiota
Cherny, Dmitry
Llorian, Miriam
Curry, Stephen
Jackson, Richard J.
Smith, Christopher W.J.
The organization of RNA contacts by PTB for regulation of FAS splicing
title The organization of RNA contacts by PTB for regulation of FAS splicing
title_full The organization of RNA contacts by PTB for regulation of FAS splicing
title_fullStr The organization of RNA contacts by PTB for regulation of FAS splicing
title_full_unstemmed The organization of RNA contacts by PTB for regulation of FAS splicing
title_short The organization of RNA contacts by PTB for regulation of FAS splicing
title_sort organization of rna contacts by ptb for regulation of fas splicing
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117754/
https://www.ncbi.nlm.nih.gov/pubmed/24957602
http://dx.doi.org/10.1093/nar/gku519
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