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Genomic mapping of cAMP receptor protein (CRP(Mt)) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRP(Mt) as a transcription factor

Chromatin immunoprecipitation identified 191 binding sites of Mycobacterium tuberculosis cAMP receptor protein (CRP(Mt)) at endogenous expression levels using a specific α-CRP(Mt) antibody. Under these native conditions an equal distribution between intragenic and intergenic locations was observed....

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Autores principales: Kahramanoglou, Christina, Cortes, Teresa, Matange, Nishad, Hunt, Debbie M., Visweswariah, Sandhya S., Young, Douglas B., Buxton, Roger S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117774/
https://www.ncbi.nlm.nih.gov/pubmed/24957601
http://dx.doi.org/10.1093/nar/gku548
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author Kahramanoglou, Christina
Cortes, Teresa
Matange, Nishad
Hunt, Debbie M.
Visweswariah, Sandhya S.
Young, Douglas B.
Buxton, Roger S.
author_facet Kahramanoglou, Christina
Cortes, Teresa
Matange, Nishad
Hunt, Debbie M.
Visweswariah, Sandhya S.
Young, Douglas B.
Buxton, Roger S.
author_sort Kahramanoglou, Christina
collection PubMed
description Chromatin immunoprecipitation identified 191 binding sites of Mycobacterium tuberculosis cAMP receptor protein (CRP(Mt)) at endogenous expression levels using a specific α-CRP(Mt) antibody. Under these native conditions an equal distribution between intragenic and intergenic locations was observed. CRP(Mt) binding overlapped a palindromic consensus sequence. Analysis by RNA sequencing revealed widespread changes in transcriptional profile in a mutant strain lacking CRP(Mt) during exponential growth, and in response to nutrient starvation. Differential expression of genes with a CRP(Mt)-binding site represented only a minor portion of this transcriptional reprogramming with ∼19% of those representing transcriptional regulators potentially controlled by CRP(Mt). The subset of genes that are differentially expressed in the deletion mutant under both culture conditions conformed to a pattern resembling canonical CRP regulation in Escherichia coli, with binding close to the transcriptional start site associated with repression and upstream binding with activation. CRP(Mt) can function as a classical transcription factor in M. tuberculosis, though this occurs at only a subset of CRP(Mt)-binding sites.
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spelling pubmed-41177742014-08-15 Genomic mapping of cAMP receptor protein (CRP(Mt)) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRP(Mt) as a transcription factor Kahramanoglou, Christina Cortes, Teresa Matange, Nishad Hunt, Debbie M. Visweswariah, Sandhya S. Young, Douglas B. Buxton, Roger S. Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Chromatin immunoprecipitation identified 191 binding sites of Mycobacterium tuberculosis cAMP receptor protein (CRP(Mt)) at endogenous expression levels using a specific α-CRP(Mt) antibody. Under these native conditions an equal distribution between intragenic and intergenic locations was observed. CRP(Mt) binding overlapped a palindromic consensus sequence. Analysis by RNA sequencing revealed widespread changes in transcriptional profile in a mutant strain lacking CRP(Mt) during exponential growth, and in response to nutrient starvation. Differential expression of genes with a CRP(Mt)-binding site represented only a minor portion of this transcriptional reprogramming with ∼19% of those representing transcriptional regulators potentially controlled by CRP(Mt). The subset of genes that are differentially expressed in the deletion mutant under both culture conditions conformed to a pattern resembling canonical CRP regulation in Escherichia coli, with binding close to the transcriptional start site associated with repression and upstream binding with activation. CRP(Mt) can function as a classical transcription factor in M. tuberculosis, though this occurs at only a subset of CRP(Mt)-binding sites. Oxford University Press 2014-09-01 2014-06-21 /pmc/articles/PMC4117774/ /pubmed/24957601 http://dx.doi.org/10.1093/nar/gku548 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene regulation, Chromatin and Epigenetics
Kahramanoglou, Christina
Cortes, Teresa
Matange, Nishad
Hunt, Debbie M.
Visweswariah, Sandhya S.
Young, Douglas B.
Buxton, Roger S.
Genomic mapping of cAMP receptor protein (CRP(Mt)) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRP(Mt) as a transcription factor
title Genomic mapping of cAMP receptor protein (CRP(Mt)) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRP(Mt) as a transcription factor
title_full Genomic mapping of cAMP receptor protein (CRP(Mt)) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRP(Mt) as a transcription factor
title_fullStr Genomic mapping of cAMP receptor protein (CRP(Mt)) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRP(Mt) as a transcription factor
title_full_unstemmed Genomic mapping of cAMP receptor protein (CRP(Mt)) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRP(Mt) as a transcription factor
title_short Genomic mapping of cAMP receptor protein (CRP(Mt)) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRP(Mt) as a transcription factor
title_sort genomic mapping of camp receptor protein (crp(mt)) in mycobacterium tuberculosis: relation to transcriptional start sites and the role of crp(mt) as a transcription factor
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117774/
https://www.ncbi.nlm.nih.gov/pubmed/24957601
http://dx.doi.org/10.1093/nar/gku548
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