Cargando…
Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties
The Escherichia coli H-NS protein is a major nucleoid-associated protein that is involved in chromosomal DNA packaging and gene regulatory functions. These biological processes are intimately related to the DNA supercoiling state and thus suggest a direct relationship between H-NS binding and DNA su...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117784/ https://www.ncbi.nlm.nih.gov/pubmed/24990375 http://dx.doi.org/10.1093/nar/gku566 |
_version_ | 1782328748536758272 |
---|---|
author | Lim, Ci Ji Kenney, Linda J. Yan, Jie |
author_facet | Lim, Ci Ji Kenney, Linda J. Yan, Jie |
author_sort | Lim, Ci Ji |
collection | PubMed |
description | The Escherichia coli H-NS protein is a major nucleoid-associated protein that is involved in chromosomal DNA packaging and gene regulatory functions. These biological processes are intimately related to the DNA supercoiling state and thus suggest a direct relationship between H-NS binding and DNA supercoiling. Here, we show that H-NS, which has two distinct DNA-binding modes, is able to differentially regulate DNA supercoiling. H-NS DNA-stiffening mode caused by nucleoprotein filament formation is able to suppress DNA plectoneme formation during DNA supercoiling. In contrast, when H-NS is in its DNA-bridging mode, it is able to promote DNA plectoneme formation during DNA supercoiling. In addition, the DNA-bridging mode is able to block twists diffusion thus trapping DNA in supercoiled domains. Overall, this work reveals the mechanical interplay between H-NS and DNA supercoiling which provides insights to H-NS organization of chromosomal DNA based on its two distinct DNA architectural properties. |
format | Online Article Text |
id | pubmed-4117784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41177842014-08-15 Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties Lim, Ci Ji Kenney, Linda J. Yan, Jie Nucleic Acids Res Gene regulation, Chromatin and Epigenetics The Escherichia coli H-NS protein is a major nucleoid-associated protein that is involved in chromosomal DNA packaging and gene regulatory functions. These biological processes are intimately related to the DNA supercoiling state and thus suggest a direct relationship between H-NS binding and DNA supercoiling. Here, we show that H-NS, which has two distinct DNA-binding modes, is able to differentially regulate DNA supercoiling. H-NS DNA-stiffening mode caused by nucleoprotein filament formation is able to suppress DNA plectoneme formation during DNA supercoiling. In contrast, when H-NS is in its DNA-bridging mode, it is able to promote DNA plectoneme formation during DNA supercoiling. In addition, the DNA-bridging mode is able to block twists diffusion thus trapping DNA in supercoiled domains. Overall, this work reveals the mechanical interplay between H-NS and DNA supercoiling which provides insights to H-NS organization of chromosomal DNA based on its two distinct DNA architectural properties. Oxford University Press 2014-09-01 2014-07-18 /pmc/articles/PMC4117784/ /pubmed/24990375 http://dx.doi.org/10.1093/nar/gku566 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene regulation, Chromatin and Epigenetics Lim, Ci Ji Kenney, Linda J. Yan, Jie Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties |
title | Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties |
title_full | Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties |
title_fullStr | Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties |
title_full_unstemmed | Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties |
title_short | Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties |
title_sort | single-molecule studies on the mechanical interplay between dna supercoiling and h-ns dna architectural properties |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117784/ https://www.ncbi.nlm.nih.gov/pubmed/24990375 http://dx.doi.org/10.1093/nar/gku566 |
work_keys_str_mv | AT limciji singlemoleculestudiesonthemechanicalinterplaybetweendnasupercoilingandhnsdnaarchitecturalproperties AT kenneylindaj singlemoleculestudiesonthemechanicalinterplaybetweendnasupercoilingandhnsdnaarchitecturalproperties AT yanjie singlemoleculestudiesonthemechanicalinterplaybetweendnasupercoilingandhnsdnaarchitecturalproperties |