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Hypermethylated-capped selenoprotein mRNAs in mammals

Mammalian mRNAs are generated by complex and coordinated biogenesis pathways and acquire 5′-end m(7)G caps that play fundamental roles in processing and translation. Here we show that several selenoprotein mRNAs are not recognized efficiently by translation initiation factor eIF4E because they bear...

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Autores principales: Wurth, Laurence, Gribling-Burrer, Anne-Sophie, Verheggen, Céline, Leichter, Michael, Takeuchi, Akiko, Baudrey, Stéphanie, Martin, Franck, Krol, Alain, Bertrand, Edouard, Allmang, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117793/
https://www.ncbi.nlm.nih.gov/pubmed/25013170
http://dx.doi.org/10.1093/nar/gku580
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author Wurth, Laurence
Gribling-Burrer, Anne-Sophie
Verheggen, Céline
Leichter, Michael
Takeuchi, Akiko
Baudrey, Stéphanie
Martin, Franck
Krol, Alain
Bertrand, Edouard
Allmang, Christine
author_facet Wurth, Laurence
Gribling-Burrer, Anne-Sophie
Verheggen, Céline
Leichter, Michael
Takeuchi, Akiko
Baudrey, Stéphanie
Martin, Franck
Krol, Alain
Bertrand, Edouard
Allmang, Christine
author_sort Wurth, Laurence
collection PubMed
description Mammalian mRNAs are generated by complex and coordinated biogenesis pathways and acquire 5′-end m(7)G caps that play fundamental roles in processing and translation. Here we show that several selenoprotein mRNAs are not recognized efficiently by translation initiation factor eIF4E because they bear a hypermethylated cap. This cap modification is acquired via a 5′-end maturation pathway similar to that of the small nucle(ol)ar RNAs (sn- and snoRNAs). Our findings also establish that the trimethylguanosine synthase 1 (Tgs1) interacts with selenoprotein mRNAs for cap hypermethylation and that assembly chaperones and core proteins devoted to sn- and snoRNP maturation contribute to recruiting Tgs1 to selenoprotein mRNPs. We further demonstrate that the hypermethylated-capped selenoprotein mRNAs localize to the cytoplasm, are associated with polysomes and thus translated. Moreover, we found that the activity of Tgs1, but not of eIF4E, is required for the synthesis of the GPx1 selenoprotein in vivo.
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spelling pubmed-41177932014-08-15 Hypermethylated-capped selenoprotein mRNAs in mammals Wurth, Laurence Gribling-Burrer, Anne-Sophie Verheggen, Céline Leichter, Michael Takeuchi, Akiko Baudrey, Stéphanie Martin, Franck Krol, Alain Bertrand, Edouard Allmang, Christine Nucleic Acids Res RNA Mammalian mRNAs are generated by complex and coordinated biogenesis pathways and acquire 5′-end m(7)G caps that play fundamental roles in processing and translation. Here we show that several selenoprotein mRNAs are not recognized efficiently by translation initiation factor eIF4E because they bear a hypermethylated cap. This cap modification is acquired via a 5′-end maturation pathway similar to that of the small nucle(ol)ar RNAs (sn- and snoRNAs). Our findings also establish that the trimethylguanosine synthase 1 (Tgs1) interacts with selenoprotein mRNAs for cap hypermethylation and that assembly chaperones and core proteins devoted to sn- and snoRNP maturation contribute to recruiting Tgs1 to selenoprotein mRNPs. We further demonstrate that the hypermethylated-capped selenoprotein mRNAs localize to the cytoplasm, are associated with polysomes and thus translated. Moreover, we found that the activity of Tgs1, but not of eIF4E, is required for the synthesis of the GPx1 selenoprotein in vivo. Oxford University Press 2014-09-01 2014-07-10 /pmc/articles/PMC4117793/ /pubmed/25013170 http://dx.doi.org/10.1093/nar/gku580 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle RNA
Wurth, Laurence
Gribling-Burrer, Anne-Sophie
Verheggen, Céline
Leichter, Michael
Takeuchi, Akiko
Baudrey, Stéphanie
Martin, Franck
Krol, Alain
Bertrand, Edouard
Allmang, Christine
Hypermethylated-capped selenoprotein mRNAs in mammals
title Hypermethylated-capped selenoprotein mRNAs in mammals
title_full Hypermethylated-capped selenoprotein mRNAs in mammals
title_fullStr Hypermethylated-capped selenoprotein mRNAs in mammals
title_full_unstemmed Hypermethylated-capped selenoprotein mRNAs in mammals
title_short Hypermethylated-capped selenoprotein mRNAs in mammals
title_sort hypermethylated-capped selenoprotein mrnas in mammals
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117793/
https://www.ncbi.nlm.nih.gov/pubmed/25013170
http://dx.doi.org/10.1093/nar/gku580
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