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Evaluating bias-reducing protocols for RNA sequencing library preparation

BACKGROUND: Next-generation sequencing does not yield fully unbiased estimates for read abundance, which may impact on the conclusions that can be drawn from sequencing data. The ligation step in RNA sequencing library generation is a known source of bias, motivating developments in enzyme technolog...

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Detalles Bibliográficos
Autores principales:	Jackson, Thomas J, Spriggs, Ruth V, Burgoyne, Nicholas J, Jones, Carolyn, Willis, Anne E
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117970/ https://www.ncbi.nlm.nih.gov/pubmed/25001197 http://dx.doi.org/10.1186/1471-2164-15-569

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