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A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
BACKGROUND: Microinjection of clustered regulatory interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-related RNA and DNA into fertilized eggs is a novel approach for creating gene-modified mice. Blastocysts obtained just before implantation may be appropriate for testi...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118159/ https://www.ncbi.nlm.nih.gov/pubmed/25042988 http://dx.doi.org/10.1186/1472-6750-14-69 |