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Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface

In this work we report on an electrochemical biosensor for the determination of the S100B protein. The His-tagged VC1 domains of Receptors for Advanced Glycation End (RAGE) products used as analytically active molecules were covalently immobilized on a monolayer of a thiol derivative of pentetic aci...

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Autores principales: Mikuła, Edyta, Wysłouch-Cieszyńska, Aleksandra, Zhukova, Liliya, Puchalska, Monika, Verwilst, Peter, Dehaen, Wim, Radecki, Jerzy, Radecka, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118347/
https://www.ncbi.nlm.nih.gov/pubmed/24940866
http://dx.doi.org/10.3390/s140610650
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author Mikuła, Edyta
Wysłouch-Cieszyńska, Aleksandra
Zhukova, Liliya
Puchalska, Monika
Verwilst, Peter
Dehaen, Wim
Radecki, Jerzy
Radecka, Hanna
author_facet Mikuła, Edyta
Wysłouch-Cieszyńska, Aleksandra
Zhukova, Liliya
Puchalska, Monika
Verwilst, Peter
Dehaen, Wim
Radecki, Jerzy
Radecka, Hanna
author_sort Mikuła, Edyta
collection PubMed
description In this work we report on an electrochemical biosensor for the determination of the S100B protein. The His-tagged VC1 domains of Receptors for Advanced Glycation End (RAGE) products used as analytically active molecules were covalently immobilized on a monolayer of a thiol derivative of pentetic acid (DPTA) complex with Cu(II) deposited on a gold electrode surface. The recognition processes between the RAGE VC1 domain and the S100B protein results in changes in the redox activity of the DPTA-Cu(II) centres which were measured by Osteryoung square-wave voltammetry (OSWV). In order to verify whether the observed analytical signal originates from the recognition process between the His(6)–RAGE VC1 domains and the S100B protein, the electrode modified with the His(6)–RAGE C2 and His(6)–RAGE VC1 deleted domains which have no ability to bind S100B peptides were applied. The proposed biosensor was quite sensitive, with a detection limit of 0.52 pM recorded in the buffer solution. The presence of diluted human plasma and 10 nM Aβ(1-40) have no influence on the biosensor performance.
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spelling pubmed-41183472014-08-01 Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface Mikuła, Edyta Wysłouch-Cieszyńska, Aleksandra Zhukova, Liliya Puchalska, Monika Verwilst, Peter Dehaen, Wim Radecki, Jerzy Radecka, Hanna Sensors (Basel) Article In this work we report on an electrochemical biosensor for the determination of the S100B protein. The His-tagged VC1 domains of Receptors for Advanced Glycation End (RAGE) products used as analytically active molecules were covalently immobilized on a monolayer of a thiol derivative of pentetic acid (DPTA) complex with Cu(II) deposited on a gold electrode surface. The recognition processes between the RAGE VC1 domain and the S100B protein results in changes in the redox activity of the DPTA-Cu(II) centres which were measured by Osteryoung square-wave voltammetry (OSWV). In order to verify whether the observed analytical signal originates from the recognition process between the His(6)–RAGE VC1 domains and the S100B protein, the electrode modified with the His(6)–RAGE C2 and His(6)–RAGE VC1 deleted domains which have no ability to bind S100B peptides were applied. The proposed biosensor was quite sensitive, with a detection limit of 0.52 pM recorded in the buffer solution. The presence of diluted human plasma and 10 nM Aβ(1-40) have no influence on the biosensor performance. MDPI 2014-06-17 /pmc/articles/PMC4118347/ /pubmed/24940866 http://dx.doi.org/10.3390/s140610650 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Mikuła, Edyta
Wysłouch-Cieszyńska, Aleksandra
Zhukova, Liliya
Puchalska, Monika
Verwilst, Peter
Dehaen, Wim
Radecki, Jerzy
Radecka, Hanna
Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface
title Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface
title_full Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface
title_fullStr Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface
title_full_unstemmed Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface
title_short Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface
title_sort voltammetric detection of s100b protein using his-tagged receptor domains for advanced glycation end products (rage) immobilized onto a gold electrode surface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118347/
https://www.ncbi.nlm.nih.gov/pubmed/24940866
http://dx.doi.org/10.3390/s140610650
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