Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis

Analogue-sensitive (as) mutants of kinases are widely used to selectively inhibit a single kinase with few off-target effects. The analogue-sensitive mutant cdc2-as of fission yeast (Schizosaccharomyces pombe) is a powerful tool to study the cell cycle, but the strain displays meiotic defects, and i...

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Detalles Bibliográficos
Autores principales: Aoi, Yuki, Kawashima, Shigehiro A., Simanis, Viesturs, Yamamoto, Masayuki, Sato, Masamitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118601/
https://www.ncbi.nlm.nih.gov/pubmed/24990387
http://dx.doi.org/10.1098/rsob.140063

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118601/
https://www.ncbi.nlm.nih.gov/pubmed/24990387
http://dx.doi.org/10.1098/rsob.140063

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