Absolute quantification by droplet digital PCR versus analog real-time PCR

Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased by 37–86%) and improved day-...

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Autores principales: Hindson, Christopher M, Chevillet, John R, Briggs, Hilary A, Gallichotte, Emily N, Ruf, Ingrid K, Hindson, Benjamin J, Vessella, Robert L, Tewari, Muneesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118677/
https://www.ncbi.nlm.nih.gov/pubmed/23995387
http://dx.doi.org/10.1038/nmeth.2633
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author Hindson, Christopher M
Chevillet, John R
Briggs, Hilary A
Gallichotte, Emily N
Ruf, Ingrid K
Hindson, Benjamin J
Vessella, Robert L
Tewari, Muneesh
author_facet Hindson, Christopher M
Chevillet, John R
Briggs, Hilary A
Gallichotte, Emily N
Ruf, Ingrid K
Hindson, Benjamin J
Vessella, Robert L
Tewari, Muneesh
author_sort Hindson, Christopher M
collection PubMed
description Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased by 37–86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis, this translated to superior diagnostic performance for identifying individuals with cancer.
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spelling pubmed-41186772014-08-01 Absolute quantification by droplet digital PCR versus analog real-time PCR Hindson, Christopher M Chevillet, John R Briggs, Hilary A Gallichotte, Emily N Ruf, Ingrid K Hindson, Benjamin J Vessella, Robert L Tewari, Muneesh Nat Methods Article Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased by 37–86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis, this translated to superior diagnostic performance for identifying individuals with cancer. 2013-09-01 2013-10 /pmc/articles/PMC4118677/ /pubmed/23995387 http://dx.doi.org/10.1038/nmeth.2633 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hindson, Christopher M
Chevillet, John R
Briggs, Hilary A
Gallichotte, Emily N
Ruf, Ingrid K
Hindson, Benjamin J
Vessella, Robert L
Tewari, Muneesh
Absolute quantification by droplet digital PCR versus analog real-time PCR
title Absolute quantification by droplet digital PCR versus analog real-time PCR
title_full Absolute quantification by droplet digital PCR versus analog real-time PCR
title_fullStr Absolute quantification by droplet digital PCR versus analog real-time PCR
title_full_unstemmed Absolute quantification by droplet digital PCR versus analog real-time PCR
title_short Absolute quantification by droplet digital PCR versus analog real-time PCR
title_sort absolute quantification by droplet digital pcr versus analog real-time pcr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118677/
https://www.ncbi.nlm.nih.gov/pubmed/23995387
http://dx.doi.org/10.1038/nmeth.2633
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