Cargando…

Absolute quantification by droplet digital PCR versus analog real-time PCR

Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased by 37–86%) and improved day-...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hindson, Christopher M, Chevillet, John R, Briggs, Hilary A, Gallichotte, Emily N, Ruf, Ingrid K, Hindson, Benjamin J, Vessella, Robert L, Tewari, Muneesh
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2013
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118677/ https://www.ncbi.nlm.nih.gov/pubmed/23995387 http://dx.doi.org/10.1038/nmeth.2633

Ejemplares similares

High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number
por: Hindson, Benjamin J., et al.
Publicado: (2011)

Quantitative Evaluation of CFTR Gene Expression: A Comparison between Relative Quantification by Real-Time PCR and Absolute Quantification by Droplet Digital PCR
por: Bruno, Sabina Maria, et al.
Publicado: (2023)

Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification
por: Pinheiro, Leonardo B., et al.
Publicado: (2011)

Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR
por: Košir, Alexandra Bogožalec, et al.
Publicado: (2017)

Droplet Digital PCR for absolute quantification and determination of proviral load of HTLV-1 and 2
por: Hedberg, Sara Thulin, et al.
Publicado: (2015)

Evaluation of Digital PCR for Absolute RNA Quantification
por: Sanders, Rebecca, et al.
Publicado: (2013)

Quantification of HEV RNA by Droplet Digital PCR
por: Nicot, Florence, et al.
Publicado: (2016)

Droplet digital PCR (ddPCR) for the detection and quantification of Ureaplasma spp.
por: Huang, Yanfang, et al.
Publicado: (2021)

A duplex droplet digital PCR assay for absolute quantification and characterization of long self-amplifying RNA
por: Casmil, Irafasha C., et al.
Publicado: (2023)

Droplet Digital PCR for Estimating Absolute Abundances of Widespread Pelagibacter Viruses
por: Martinez-Hernandez, Francisco, et al.
Publicado: (2019)

Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)
por: Koepfli, Cristian, et al.
Publicado: (2016)

A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR
por: He, Kuang, et al.
Publicado: (2019)

Comparison of Droplet Digital PCR and Seminested Real-Time PCR for Quantification of Cell-Associated HIV-1 RNA
por: Kiselinova, Maja, et al.
Publicado: (2014)

Detection and quantification of Verticillium dahliae and V. longisporum by droplet digital PCR versus quantitative real-time PCR
por: Wang, Di, et al.
Publicado: (2022)

Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR
por: Feng, Junxia, et al.
Publicado: (2023)

Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification of Aspergillus species in the Human Airway
por: Poh, Tuang Yeow, et al.
Publicado: (2020)

Absolute Quantification of Viable but Nonculturable Vibrio cholerae Using Droplet Digital PCR with Oil-Enveloped Bacterial Cells
por: Zhao, Shuo, et al.
Publicado: (2022)

Absolute quantification of Mycoplasma pneumoniae in infected patients by droplet digital PCR to track disease severity and treatment efficacy
por: Zhao, Hanqing, et al.
Publicado: (2023)

Absolute quantification of viable Vibrio cholerae in seawater samples using multiplex droplet digital PCR combined with propidium monoazide
por: Yang, Jinsong, et al.
Publicado: (2023)

Diagnostic utility of droplet digital PCR for HIV reservoir quantification
por: Trypsteen, Wim, et al.
Publicado: (2016)

Impact of variance components on reliability of absolute quantification using digital PCR
por: Jacobs, Bart KM, et al.
Publicado: (2014)

Ultrasensitive detection and quantification of viral nucleic acids with Raindance droplet digital PCR (ddPCR)
por: Long, Samuel, et al.
Publicado: (2022)

Digital Droplet PCR for the Absolute Quantification of Exon Skipping Induced by Antisense Oligonucleotides in (Pre-)Clinical Development for Duchenne Muscular Dystrophy
por: Verheul, Ruurd C., et al.
Publicado: (2016)

Digital ILC 2020
por: Hindson, Jordan
Publicado: (2020)

Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR
por: Plotka, Magdalena, et al.
Publicado: (2017)

Droplet digital PCR for the quantification of Alu methylation status in hematological malignancies
por: Orsini, Paola, et al.
Publicado: (2018)

Four human Plasmodium species quantification using droplet digital PCR
por: Srisutham, Suttipat, et al.
Publicado: (2017)

Quantification of circulating microRNAs by droplet digital PCR for cancer detection
por: Cirillo, Priscila D. R., et al.
Publicado: (2020)

Digital-Droplet PCR for Quantification of CD19-Directed CAR T-Cells
por: Mika, Thomas, et al.
Publicado: (2020)

HIV reservoir quantification by five-target multiplex droplet digital PCR
por: Levy, Claire N., et al.
Publicado: (2021)

Clinical Application of Droplet Digital PCR for Hepatitis Delta Virus Quantification
por: Olivero, Antonella, et al.
Publicado: (2022)

Simultaneous Giant Virus and Virophage Quantification Using Droplet Digital PCR
por: del Arco, Ana, et al.
Publicado: (2022)

Development of the Droplet Digital PCR Method for the Detection and Quantification of Erwinia pyrifoliae
por: He, Lin, et al.
Publicado: (2023)

Absolute quantification of SARS-CoV-2 with Clarity Plus™ digital PCR
por: Tan, Shawn Yi Han, et al.
Publicado: (2022)

Droplet digital PCR versus real-time PCR for in-house validation of porcine detection and quantification protocol: An artificial recombinant plasmid approach
por: Nuraeni, Umi, et al.
Publicado: (2023)

Droplet Digital PCR (ddPCR) Analysis for the Detection and Quantification of Cow DNA in Buffalo Mozzarella Cheese
por: Cutarelli, Anna, et al.
Publicado: (2021)

Assessment of the real-time PCR and different digital PCR platforms for DNA quantification
por: Pavšič, Jernej, et al.
Publicado: (2015)

Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR
por: Maheshwari, Yogita, et al.
Publicado: (2017)

Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces
por: Verhaegen, Bavo, et al.
Publicado: (2016)

Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR
por: D’Alessandra, Yuri, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_ihco4mo4f4f6j99sr4jhekho0c