The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain

The first structure of the catalytic domain of RpfC (Rv1884), one of the resuscitation-promoting factors (RPFs) from Mycobacterium tuberculosis, is reported. The structure was solved using molecular replacement once the space group had been correctly identified as twinned P2(1) rather than the appar...

Descripción completa

Detalles Bibliográficos
Autores principales: Chauviac, Francois-Xavier, Robertson, Giles, Quay, Doris H. X., Bagnéris, Claire, Dumas, Christian, Henderson, Brian, Ward, John, Keep, Nicholas H., Cohen-Gonsaud, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2014
Materias:
Structural Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118796/
https://www.ncbi.nlm.nih.gov/pubmed/25084374
http://dx.doi.org/10.1107/S2053230X1401317X
_version_ 1782328886291333120
author Chauviac, Francois-Xavier
Robertson, Giles
Quay, Doris H. X.
Bagnéris, Claire
Dumas, Christian
Henderson, Brian
Ward, John
Keep, Nicholas H.
Cohen-Gonsaud, Martin
author_facet Chauviac, Francois-Xavier
Robertson, Giles
Quay, Doris H. X.
Bagnéris, Claire
Dumas, Christian
Henderson, Brian
Ward, John
Keep, Nicholas H.
Cohen-Gonsaud, Martin
author_sort Chauviac, Francois-Xavier
collection PubMed
description The first structure of the catalytic domain of RpfC (Rv1884), one of the resuscitation-promoting factors (RPFs) from Mycobacterium tuberculosis, is reported. The structure was solved using molecular replacement once the space group had been correctly identified as twinned P2(1) rather than the apparent C222(1) by searching for anomalous scattering sites in P1. The structure displays a very high degree of structural conservation with the previously published structures of the catalytic domains of RpfB (Rv1009) and RpfE (Rv2450). This structural conservation highlights the importance of the versatile domain composition of the RPF family.
format Online
Article
Text
id pubmed-4118796
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher International Union of Crystallography
record_format MEDLINE/PubMed
spelling pubmed-41187962014-08-15 The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain Chauviac, Francois-Xavier Robertson, Giles Quay, Doris H. X. Bagnéris, Claire Dumas, Christian Henderson, Brian Ward, John Keep, Nicholas H. Cohen-Gonsaud, Martin Acta Crystallogr F Struct Biol Commun Structural Communications The first structure of the catalytic domain of RpfC (Rv1884), one of the resuscitation-promoting factors (RPFs) from Mycobacterium tuberculosis, is reported. The structure was solved using molecular replacement once the space group had been correctly identified as twinned P2(1) rather than the apparent C222(1) by searching for anomalous scattering sites in P1. The structure displays a very high degree of structural conservation with the previously published structures of the catalytic domains of RpfB (Rv1009) and RpfE (Rv2450). This structural conservation highlights the importance of the versatile domain composition of the RPF family. International Union of Crystallography 2014-07-23 /pmc/articles/PMC4118796/ /pubmed/25084374 http://dx.doi.org/10.1107/S2053230X1401317X Text en © Chauviac et al. 2014 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Structural Communications
Chauviac, Francois-Xavier
Robertson, Giles
Quay, Doris H. X.
Bagnéris, Claire
Dumas, Christian
Henderson, Brian
Ward, John
Keep, Nicholas H.
Cohen-Gonsaud, Martin
The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain
title The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain
title_full The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain
title_fullStr The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain
title_full_unstemmed The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain
title_short The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain
title_sort rpfc (rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain
topic Structural Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118796/
https://www.ncbi.nlm.nih.gov/pubmed/25084374
http://dx.doi.org/10.1107/S2053230X1401317X
work_keys_str_mv AT chauviacfrancoisxavier therpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT robertsongiles therpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT quaydorishx therpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT bagnerisclaire therpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT dumaschristian therpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT hendersonbrian therpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT wardjohn therpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT keepnicholash therpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT cohengonsaudmartin therpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT chauviacfrancoisxavier rpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT robertsongiles rpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT quaydorishx rpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT bagnerisclaire rpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT dumaschristian rpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT hendersonbrian rpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT wardjohn rpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT keepnicholash rpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain
AT cohengonsaudmartin rpfcrv1884atomicstructureshowshighstructuralconservationwithintheresuscitationpromotingfactorcatalyticdomain

Ejemplares similares