Cargando…
Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing
Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equ...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118893/ https://www.ncbi.nlm.nih.gov/pubmed/25083787 http://dx.doi.org/10.1371/journal.pone.0103560 |
_version_ | 1782328902335594496 |
---|---|
author | Hatagishi, Etsuko Okamoto, Michiko Ohmiya, Suguru Yano, Hisakazu Hori, Toru Saito, Wakana Miki, Hiroshi Suzuki, Yasushi Saito, Reiko Yamamoto, Taro Shoji, Makoto Morisaki, Yoshihisa Sakata, Soichiro Nishimura, Hidekazu |
author_facet | Hatagishi, Etsuko Okamoto, Michiko Ohmiya, Suguru Yano, Hisakazu Hori, Toru Saito, Wakana Miki, Hiroshi Suzuki, Yasushi Saito, Reiko Yamamoto, Taro Shoji, Makoto Morisaki, Yoshihisa Sakata, Soichiro Nishimura, Hidekazu |
author_sort | Hatagishi, Etsuko |
collection | PubMed |
description | Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equipped with an air sampler to draw air and pass it through a gelatin membrane filter connected to a cone-shaped, megaphone-like device to guide the cough airflow to the membrane. The membrane was dissolved in a medium, and the viral load was measured using quantitative real-time reverse transcriptase-polymerase chain reaction and a plaque assay. The approximate viral recovery rate of this system was 10% in simulation experiments to collect and quantify the viral particles aerosolized by a nebulizer. Using this system, cough samples were collected from 56 influenza A patients. The total viral detection rate was 41% (23/56), and the viral loads varied significantly (from <10, less than the detection limit, to 2240 viral gene copies/cough). Viable viruses were detected from 3 samples with ≤18 plaque forming units per cough sample. The virus detection rates were similar among different groups of patients infected with different viral subtypes and during different influenza seasons. Among patients who did not receive antiviral treatment, viruses were detected in one of six cases in the vaccinated group and four of six cases in the unvaccinated group. We found cases with high viral titers in throat swabs or oral secretions but very low or undetectable in coughs and vice versa suggesting other possible anatomical sites where the viruses might be mixed into the cough. Our system is easy to operate, appropriate for bedside use, and is useful for comparing the viral load in cough samples from influenza patients under various conditions and settings. However, further large-scale studies are warranted to validate our results. |
format | Online Article Text |
id | pubmed-4118893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41188932014-08-04 Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing Hatagishi, Etsuko Okamoto, Michiko Ohmiya, Suguru Yano, Hisakazu Hori, Toru Saito, Wakana Miki, Hiroshi Suzuki, Yasushi Saito, Reiko Yamamoto, Taro Shoji, Makoto Morisaki, Yoshihisa Sakata, Soichiro Nishimura, Hidekazu PLoS One Research Article Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equipped with an air sampler to draw air and pass it through a gelatin membrane filter connected to a cone-shaped, megaphone-like device to guide the cough airflow to the membrane. The membrane was dissolved in a medium, and the viral load was measured using quantitative real-time reverse transcriptase-polymerase chain reaction and a plaque assay. The approximate viral recovery rate of this system was 10% in simulation experiments to collect and quantify the viral particles aerosolized by a nebulizer. Using this system, cough samples were collected from 56 influenza A patients. The total viral detection rate was 41% (23/56), and the viral loads varied significantly (from <10, less than the detection limit, to 2240 viral gene copies/cough). Viable viruses were detected from 3 samples with ≤18 plaque forming units per cough sample. The virus detection rates were similar among different groups of patients infected with different viral subtypes and during different influenza seasons. Among patients who did not receive antiviral treatment, viruses were detected in one of six cases in the vaccinated group and four of six cases in the unvaccinated group. We found cases with high viral titers in throat swabs or oral secretions but very low or undetectable in coughs and vice versa suggesting other possible anatomical sites where the viruses might be mixed into the cough. Our system is easy to operate, appropriate for bedside use, and is useful for comparing the viral load in cough samples from influenza patients under various conditions and settings. However, further large-scale studies are warranted to validate our results. Public Library of Science 2014-08-01 /pmc/articles/PMC4118893/ /pubmed/25083787 http://dx.doi.org/10.1371/journal.pone.0103560 Text en © 2014 Hatagishi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hatagishi, Etsuko Okamoto, Michiko Ohmiya, Suguru Yano, Hisakazu Hori, Toru Saito, Wakana Miki, Hiroshi Suzuki, Yasushi Saito, Reiko Yamamoto, Taro Shoji, Makoto Morisaki, Yoshihisa Sakata, Soichiro Nishimura, Hidekazu Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing |
title | Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing |
title_full | Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing |
title_fullStr | Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing |
title_full_unstemmed | Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing |
title_short | Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing |
title_sort | establishment and clinical applications of a portable system for capturing influenza viruses released through coughing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118893/ https://www.ncbi.nlm.nih.gov/pubmed/25083787 http://dx.doi.org/10.1371/journal.pone.0103560 |
work_keys_str_mv | AT hatagishietsuko establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT okamotomichiko establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT ohmiyasuguru establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT yanohisakazu establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT horitoru establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT saitowakana establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT mikihiroshi establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT suzukiyasushi establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT saitoreiko establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT yamamototaro establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT shojimakoto establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT morisakiyoshihisa establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT sakatasoichiro establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing AT nishimurahidekazu establishmentandclinicalapplicationsofaportablesystemforcapturinginfluenzavirusesreleasedthroughcoughing |