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Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing

Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equ...

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Autores principales: Hatagishi, Etsuko, Okamoto, Michiko, Ohmiya, Suguru, Yano, Hisakazu, Hori, Toru, Saito, Wakana, Miki, Hiroshi, Suzuki, Yasushi, Saito, Reiko, Yamamoto, Taro, Shoji, Makoto, Morisaki, Yoshihisa, Sakata, Soichiro, Nishimura, Hidekazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118893/
https://www.ncbi.nlm.nih.gov/pubmed/25083787
http://dx.doi.org/10.1371/journal.pone.0103560
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author Hatagishi, Etsuko
Okamoto, Michiko
Ohmiya, Suguru
Yano, Hisakazu
Hori, Toru
Saito, Wakana
Miki, Hiroshi
Suzuki, Yasushi
Saito, Reiko
Yamamoto, Taro
Shoji, Makoto
Morisaki, Yoshihisa
Sakata, Soichiro
Nishimura, Hidekazu
author_facet Hatagishi, Etsuko
Okamoto, Michiko
Ohmiya, Suguru
Yano, Hisakazu
Hori, Toru
Saito, Wakana
Miki, Hiroshi
Suzuki, Yasushi
Saito, Reiko
Yamamoto, Taro
Shoji, Makoto
Morisaki, Yoshihisa
Sakata, Soichiro
Nishimura, Hidekazu
author_sort Hatagishi, Etsuko
collection PubMed
description Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equipped with an air sampler to draw air and pass it through a gelatin membrane filter connected to a cone-shaped, megaphone-like device to guide the cough airflow to the membrane. The membrane was dissolved in a medium, and the viral load was measured using quantitative real-time reverse transcriptase-polymerase chain reaction and a plaque assay. The approximate viral recovery rate of this system was 10% in simulation experiments to collect and quantify the viral particles aerosolized by a nebulizer. Using this system, cough samples were collected from 56 influenza A patients. The total viral detection rate was 41% (23/56), and the viral loads varied significantly (from <10, less than the detection limit, to 2240 viral gene copies/cough). Viable viruses were detected from 3 samples with ≤18 plaque forming units per cough sample. The virus detection rates were similar among different groups of patients infected with different viral subtypes and during different influenza seasons. Among patients who did not receive antiviral treatment, viruses were detected in one of six cases in the vaccinated group and four of six cases in the unvaccinated group. We found cases with high viral titers in throat swabs or oral secretions but very low or undetectable in coughs and vice versa suggesting other possible anatomical sites where the viruses might be mixed into the cough. Our system is easy to operate, appropriate for bedside use, and is useful for comparing the viral load in cough samples from influenza patients under various conditions and settings. However, further large-scale studies are warranted to validate our results.
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spelling pubmed-41188932014-08-04 Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing Hatagishi, Etsuko Okamoto, Michiko Ohmiya, Suguru Yano, Hisakazu Hori, Toru Saito, Wakana Miki, Hiroshi Suzuki, Yasushi Saito, Reiko Yamamoto, Taro Shoji, Makoto Morisaki, Yoshihisa Sakata, Soichiro Nishimura, Hidekazu PLoS One Research Article Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equipped with an air sampler to draw air and pass it through a gelatin membrane filter connected to a cone-shaped, megaphone-like device to guide the cough airflow to the membrane. The membrane was dissolved in a medium, and the viral load was measured using quantitative real-time reverse transcriptase-polymerase chain reaction and a plaque assay. The approximate viral recovery rate of this system was 10% in simulation experiments to collect and quantify the viral particles aerosolized by a nebulizer. Using this system, cough samples were collected from 56 influenza A patients. The total viral detection rate was 41% (23/56), and the viral loads varied significantly (from <10, less than the detection limit, to 2240 viral gene copies/cough). Viable viruses were detected from 3 samples with ≤18 plaque forming units per cough sample. The virus detection rates were similar among different groups of patients infected with different viral subtypes and during different influenza seasons. Among patients who did not receive antiviral treatment, viruses were detected in one of six cases in the vaccinated group and four of six cases in the unvaccinated group. We found cases with high viral titers in throat swabs or oral secretions but very low or undetectable in coughs and vice versa suggesting other possible anatomical sites where the viruses might be mixed into the cough. Our system is easy to operate, appropriate for bedside use, and is useful for comparing the viral load in cough samples from influenza patients under various conditions and settings. However, further large-scale studies are warranted to validate our results. Public Library of Science 2014-08-01 /pmc/articles/PMC4118893/ /pubmed/25083787 http://dx.doi.org/10.1371/journal.pone.0103560 Text en © 2014 Hatagishi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hatagishi, Etsuko
Okamoto, Michiko
Ohmiya, Suguru
Yano, Hisakazu
Hori, Toru
Saito, Wakana
Miki, Hiroshi
Suzuki, Yasushi
Saito, Reiko
Yamamoto, Taro
Shoji, Makoto
Morisaki, Yoshihisa
Sakata, Soichiro
Nishimura, Hidekazu
Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing
title Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing
title_full Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing
title_fullStr Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing
title_full_unstemmed Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing
title_short Establishment and Clinical Applications of a Portable System for Capturing Influenza Viruses Released through Coughing
title_sort establishment and clinical applications of a portable system for capturing influenza viruses released through coughing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118893/
https://www.ncbi.nlm.nih.gov/pubmed/25083787
http://dx.doi.org/10.1371/journal.pone.0103560
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