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Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties

Cereal Brittle1 protein has been demonstrated to be involved in the ADP-Glc transport into endosperm plastids, and plays vital roles in the biosynthesis of starch. In this study, the genomic sequences of the ZmBT1 gene in 80 elite maize inbred lines were obtained, and the nucleotide polymorphisms an...

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Autores principales: Xu, Shuhui, Yang, Zefeng, Zhang, Enying, Jiang, Ying, Pan, Liang, Chen, Qing, Xie, Zhengwen, Xu, Chenwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118901/
https://www.ncbi.nlm.nih.gov/pubmed/25084007
http://dx.doi.org/10.1371/journal.pone.0103627
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author Xu, Shuhui
Yang, Zefeng
Zhang, Enying
Jiang, Ying
Pan, Liang
Chen, Qing
Xie, Zhengwen
Xu, Chenwu
author_facet Xu, Shuhui
Yang, Zefeng
Zhang, Enying
Jiang, Ying
Pan, Liang
Chen, Qing
Xie, Zhengwen
Xu, Chenwu
author_sort Xu, Shuhui
collection PubMed
description Cereal Brittle1 protein has been demonstrated to be involved in the ADP-Glc transport into endosperm plastids, and plays vital roles in the biosynthesis of starch. In this study, the genomic sequences of the ZmBT1 gene in 80 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. A total of 30 variants, including 22 SNPs and 8 indels, were detected from the full sequences of this gene. Among these polymorphic sites, 9 SNPs and 2 indels were found to be located in the coding region. The polymorphisms of CDS sequences classified the maize ZmBT1 gene into 6 haplotypes, which encode 6 different ZmBT1 proteins. Neutrality tests revealed a decrease in population size and/or balancing selection on the maize ZmBT1 locus. To detect the association between sequence variations of this gene and the starch physicochemical properties, 7 pasting and 4 gelatinization traits of starch were measured for the tested inbred lines using rapid visco analyzer (RVA) and differential scanning calorimeter (DSC), respectively. The result of association analysis revealed that an indel in the coding region was significantly associated with the phenotypic variation of starch gelatinization enthalpy.
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spelling pubmed-41189012014-08-04 Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties Xu, Shuhui Yang, Zefeng Zhang, Enying Jiang, Ying Pan, Liang Chen, Qing Xie, Zhengwen Xu, Chenwu PLoS One Research Article Cereal Brittle1 protein has been demonstrated to be involved in the ADP-Glc transport into endosperm plastids, and plays vital roles in the biosynthesis of starch. In this study, the genomic sequences of the ZmBT1 gene in 80 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. A total of 30 variants, including 22 SNPs and 8 indels, were detected from the full sequences of this gene. Among these polymorphic sites, 9 SNPs and 2 indels were found to be located in the coding region. The polymorphisms of CDS sequences classified the maize ZmBT1 gene into 6 haplotypes, which encode 6 different ZmBT1 proteins. Neutrality tests revealed a decrease in population size and/or balancing selection on the maize ZmBT1 locus. To detect the association between sequence variations of this gene and the starch physicochemical properties, 7 pasting and 4 gelatinization traits of starch were measured for the tested inbred lines using rapid visco analyzer (RVA) and differential scanning calorimeter (DSC), respectively. The result of association analysis revealed that an indel in the coding region was significantly associated with the phenotypic variation of starch gelatinization enthalpy. Public Library of Science 2014-08-01 /pmc/articles/PMC4118901/ /pubmed/25084007 http://dx.doi.org/10.1371/journal.pone.0103627 Text en © 2014 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xu, Shuhui
Yang, Zefeng
Zhang, Enying
Jiang, Ying
Pan, Liang
Chen, Qing
Xie, Zhengwen
Xu, Chenwu
Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties
title Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties
title_full Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties
title_fullStr Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties
title_full_unstemmed Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties
title_short Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties
title_sort nucleotide diversity of maize zmbt1 gene and association with starch physicochemical properties
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118901/
https://www.ncbi.nlm.nih.gov/pubmed/25084007
http://dx.doi.org/10.1371/journal.pone.0103627
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