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Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties
Cereal Brittle1 protein has been demonstrated to be involved in the ADP-Glc transport into endosperm plastids, and plays vital roles in the biosynthesis of starch. In this study, the genomic sequences of the ZmBT1 gene in 80 elite maize inbred lines were obtained, and the nucleotide polymorphisms an...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118901/ https://www.ncbi.nlm.nih.gov/pubmed/25084007 http://dx.doi.org/10.1371/journal.pone.0103627 |
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author | Xu, Shuhui Yang, Zefeng Zhang, Enying Jiang, Ying Pan, Liang Chen, Qing Xie, Zhengwen Xu, Chenwu |
author_facet | Xu, Shuhui Yang, Zefeng Zhang, Enying Jiang, Ying Pan, Liang Chen, Qing Xie, Zhengwen Xu, Chenwu |
author_sort | Xu, Shuhui |
collection | PubMed |
description | Cereal Brittle1 protein has been demonstrated to be involved in the ADP-Glc transport into endosperm plastids, and plays vital roles in the biosynthesis of starch. In this study, the genomic sequences of the ZmBT1 gene in 80 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. A total of 30 variants, including 22 SNPs and 8 indels, were detected from the full sequences of this gene. Among these polymorphic sites, 9 SNPs and 2 indels were found to be located in the coding region. The polymorphisms of CDS sequences classified the maize ZmBT1 gene into 6 haplotypes, which encode 6 different ZmBT1 proteins. Neutrality tests revealed a decrease in population size and/or balancing selection on the maize ZmBT1 locus. To detect the association between sequence variations of this gene and the starch physicochemical properties, 7 pasting and 4 gelatinization traits of starch were measured for the tested inbred lines using rapid visco analyzer (RVA) and differential scanning calorimeter (DSC), respectively. The result of association analysis revealed that an indel in the coding region was significantly associated with the phenotypic variation of starch gelatinization enthalpy. |
format | Online Article Text |
id | pubmed-4118901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41189012014-08-04 Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties Xu, Shuhui Yang, Zefeng Zhang, Enying Jiang, Ying Pan, Liang Chen, Qing Xie, Zhengwen Xu, Chenwu PLoS One Research Article Cereal Brittle1 protein has been demonstrated to be involved in the ADP-Glc transport into endosperm plastids, and plays vital roles in the biosynthesis of starch. In this study, the genomic sequences of the ZmBT1 gene in 80 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. A total of 30 variants, including 22 SNPs and 8 indels, were detected from the full sequences of this gene. Among these polymorphic sites, 9 SNPs and 2 indels were found to be located in the coding region. The polymorphisms of CDS sequences classified the maize ZmBT1 gene into 6 haplotypes, which encode 6 different ZmBT1 proteins. Neutrality tests revealed a decrease in population size and/or balancing selection on the maize ZmBT1 locus. To detect the association between sequence variations of this gene and the starch physicochemical properties, 7 pasting and 4 gelatinization traits of starch were measured for the tested inbred lines using rapid visco analyzer (RVA) and differential scanning calorimeter (DSC), respectively. The result of association analysis revealed that an indel in the coding region was significantly associated with the phenotypic variation of starch gelatinization enthalpy. Public Library of Science 2014-08-01 /pmc/articles/PMC4118901/ /pubmed/25084007 http://dx.doi.org/10.1371/journal.pone.0103627 Text en © 2014 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xu, Shuhui Yang, Zefeng Zhang, Enying Jiang, Ying Pan, Liang Chen, Qing Xie, Zhengwen Xu, Chenwu Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties |
title | Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties |
title_full | Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties |
title_fullStr | Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties |
title_full_unstemmed | Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties |
title_short | Nucleotide Diversity of Maize ZmBT1 Gene and Association with Starch Physicochemical Properties |
title_sort | nucleotide diversity of maize zmbt1 gene and association with starch physicochemical properties |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118901/ https://www.ncbi.nlm.nih.gov/pubmed/25084007 http://dx.doi.org/10.1371/journal.pone.0103627 |
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