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Quantification of eggshell microstructure using X-ray micro computed tomography

1. X-ray microcomputed tomography can be used to produce rapid, fully analysable, three-dimensional images of biological and other materials without the need for complex or tedious sample preparation and sectioning. We describe the use of this technique to visualise and analyse the microstructure of...

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Detalles Bibliográficos
Autores principales: Riley, A., Sturrock, C. J., Mooney, S. J., Luck, M. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118947/
https://www.ncbi.nlm.nih.gov/pubmed/24875292
http://dx.doi.org/10.1080/00071668.2014.924093
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author Riley, A.
Sturrock, C. J.
Mooney, S. J.
Luck, M. R.
author_facet Riley, A.
Sturrock, C. J.
Mooney, S. J.
Luck, M. R.
author_sort Riley, A.
collection PubMed
description 1. X-ray microcomputed tomography can be used to produce rapid, fully analysable, three-dimensional images of biological and other materials without the need for complex or tedious sample preparation and sectioning. We describe the use of this technique to visualise and analyse the microstructure of fragments of shell taken from three regions of chicken eggs (sharp pole, blunt pole and equatorial region). 2. Two- and three-dimensional images and data were obtained at a resolution of 1.5 microns. The images were analysed to provide measurements of shell thickness, the spacial density of mammillary bodies, the frequency, shape, volume and effective diameter of individual pore spaces, and the intrinsic sponginess (proportion of non-X-ray dense material formed by vesicles) of the shell matrix. Measurements of these parameters were comparable with those derived by traditional methods and reported in the literature. 3. The advantages of using this technology for the quantification of eggshell microstructural parameters and its potential application for commercial, research and other purposes are discussed.
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spelling pubmed-41189472014-08-26 Quantification of eggshell microstructure using X-ray micro computed tomography Riley, A. Sturrock, C. J. Mooney, S. J. Luck, M. R. Br Poult Sci Research Article 1. X-ray microcomputed tomography can be used to produce rapid, fully analysable, three-dimensional images of biological and other materials without the need for complex or tedious sample preparation and sectioning. We describe the use of this technique to visualise and analyse the microstructure of fragments of shell taken from three regions of chicken eggs (sharp pole, blunt pole and equatorial region). 2. Two- and three-dimensional images and data were obtained at a resolution of 1.5 microns. The images were analysed to provide measurements of shell thickness, the spacial density of mammillary bodies, the frequency, shape, volume and effective diameter of individual pore spaces, and the intrinsic sponginess (proportion of non-X-ray dense material formed by vesicles) of the shell matrix. Measurements of these parameters were comparable with those derived by traditional methods and reported in the literature. 3. The advantages of using this technology for the quantification of eggshell microstructural parameters and its potential application for commercial, research and other purposes are discussed. Taylor & Francis 2014-07-04 2014-06 /pmc/articles/PMC4118947/ /pubmed/24875292 http://dx.doi.org/10.1080/00071668.2014.924093 Text en © 2014 The Author(s). Published by Taylor & Francis. http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Taylor & Francis journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This is an Open Access article. Non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly attributed, cited, and is not altered, transformed, or built upon in any way, is permitted. The moral rights of the named author(s) have been asserted.
spellingShingle Research Article
Riley, A.
Sturrock, C. J.
Mooney, S. J.
Luck, M. R.
Quantification of eggshell microstructure using X-ray micro computed tomography
title Quantification of eggshell microstructure using X-ray micro computed tomography
title_full Quantification of eggshell microstructure using X-ray micro computed tomography
title_fullStr Quantification of eggshell microstructure using X-ray micro computed tomography
title_full_unstemmed Quantification of eggshell microstructure using X-ray micro computed tomography
title_short Quantification of eggshell microstructure using X-ray micro computed tomography
title_sort quantification of eggshell microstructure using x-ray micro computed tomography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118947/
https://www.ncbi.nlm.nih.gov/pubmed/24875292
http://dx.doi.org/10.1080/00071668.2014.924093
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