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Frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans

Objectives: The aim of this study was to evaluate the possibility of technique simplification for cytology slides in order to evaluate the frequency of micronuclei (FMic) and conduct a experiment looking to know the FMic of oral epithelial cells of healthy volunteers exposed to mate tea (Ilex paragu...

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Autores principales: Bortoluzzi, Marcelo C., Campagnoli, Eduardo B., Milan, José R., Reinheimer, Angélica, Masson, Maicon, Capella, Diogo L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medicina Oral S.L. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4119309/
https://www.ncbi.nlm.nih.gov/pubmed/24608213
http://dx.doi.org/10.4317/medoral.19570
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author Bortoluzzi, Marcelo C.
Campagnoli, Eduardo B.
Milan, José R.
Reinheimer, Angélica
Masson, Maicon
Capella, Diogo L.
author_facet Bortoluzzi, Marcelo C.
Campagnoli, Eduardo B.
Milan, José R.
Reinheimer, Angélica
Masson, Maicon
Capella, Diogo L.
author_sort Bortoluzzi, Marcelo C.
collection PubMed
description Objectives: The aim of this study was to evaluate the possibility of technique simplification for cytology slides in order to evaluate the frequency of micronuclei (FMic) and conduct a experiment looking to know the FMic of oral epithelial cells of healthy volunteers exposed to mate tea (Ilex paraguarariensis). Material and Methods: This is a laboratorial and nonrandomized trial (quasi-experiment), where the nonusers subjects were exposed to mate-tea, consumed in the traditional way, two drinks, two times a day for a single week. Two cytology of exfoliated epithelial cells were obtained before and after the mate tea exposition. Results: The sample was composed by 10 volunteers. The age ranged from 18 to 33 years (Mean 23; SD5.5). The use of mate tea did not showed significant variation in the FMic (Wilcoxon Signed Ranks Test p= .24). Conclusions: The proposed technique simplification showed to be reliable, without losses when compared to the conventional technique and with the advantage of eliminate toxic substances, becoming simple and practical tool for research in dentistry. The acute exposure to mate tea did not induce an increase of FMic in exfoliated buccal cells of healthy nondrinkers and nonsmokers subjects and may not have genotoxic effect. More human studies are needed before a conclusion can be made on the oral carcinogenic risk of mate tea to humans. Key words:Micronucleus, Oral Cancer, Cytology, Mate tea, Ilex paraguariensis.
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spelling pubmed-41193092014-08-14 Frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans Bortoluzzi, Marcelo C. Campagnoli, Eduardo B. Milan, José R. Reinheimer, Angélica Masson, Maicon Capella, Diogo L. Med Oral Patol Oral Cir Bucal Research Objectives: The aim of this study was to evaluate the possibility of technique simplification for cytology slides in order to evaluate the frequency of micronuclei (FMic) and conduct a experiment looking to know the FMic of oral epithelial cells of healthy volunteers exposed to mate tea (Ilex paraguarariensis). Material and Methods: This is a laboratorial and nonrandomized trial (quasi-experiment), where the nonusers subjects were exposed to mate-tea, consumed in the traditional way, two drinks, two times a day for a single week. Two cytology of exfoliated epithelial cells were obtained before and after the mate tea exposition. Results: The sample was composed by 10 volunteers. The age ranged from 18 to 33 years (Mean 23; SD5.5). The use of mate tea did not showed significant variation in the FMic (Wilcoxon Signed Ranks Test p= .24). Conclusions: The proposed technique simplification showed to be reliable, without losses when compared to the conventional technique and with the advantage of eliminate toxic substances, becoming simple and practical tool for research in dentistry. The acute exposure to mate tea did not induce an increase of FMic in exfoliated buccal cells of healthy nondrinkers and nonsmokers subjects and may not have genotoxic effect. More human studies are needed before a conclusion can be made on the oral carcinogenic risk of mate tea to humans. Key words:Micronucleus, Oral Cancer, Cytology, Mate tea, Ilex paraguariensis. Medicina Oral S.L. 2014-07 2014-03-08 /pmc/articles/PMC4119309/ /pubmed/24608213 http://dx.doi.org/10.4317/medoral.19570 Text en Copyright: © 2014 Medicina Oral S.L. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Bortoluzzi, Marcelo C.
Campagnoli, Eduardo B.
Milan, José R.
Reinheimer, Angélica
Masson, Maicon
Capella, Diogo L.
Frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans
title Frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans
title_full Frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans
title_fullStr Frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans
title_full_unstemmed Frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans
title_short Frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans
title_sort frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4119309/
https://www.ncbi.nlm.nih.gov/pubmed/24608213
http://dx.doi.org/10.4317/medoral.19570
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