Cargando…
Population genomics of parallel hybrid zones in the mimetic butterflies, H. melpomene and H. erato
Hybrid zones can be valuable tools for studying evolution and identifying genomic regions responsible for adaptive divergence and underlying phenotypic variation. Hybrid zones between subspecies of Heliconius butterflies can be very narrow and are maintained by strong selection acting on color patte...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120085/ https://www.ncbi.nlm.nih.gov/pubmed/24823669 http://dx.doi.org/10.1101/gr.169292.113 |
_version_ | 1782329038620065792 |
---|---|
author | Nadeau, Nicola J. Ruiz, Mayté Salazar, Patricio Counterman, Brian Medina, Jose Alejandro Ortiz-Zuazaga, Humberto Morrison, Anna McMillan, W. Owen Jiggins, Chris D. Papa, Riccardo |
author_facet | Nadeau, Nicola J. Ruiz, Mayté Salazar, Patricio Counterman, Brian Medina, Jose Alejandro Ortiz-Zuazaga, Humberto Morrison, Anna McMillan, W. Owen Jiggins, Chris D. Papa, Riccardo |
author_sort | Nadeau, Nicola J. |
collection | PubMed |
description | Hybrid zones can be valuable tools for studying evolution and identifying genomic regions responsible for adaptive divergence and underlying phenotypic variation. Hybrid zones between subspecies of Heliconius butterflies can be very narrow and are maintained by strong selection acting on color pattern. The comimetic species, H. erato and H. melpomene, have parallel hybrid zones in which both species undergo a change from one color pattern form to another. We use restriction-associated DNA sequencing to obtain several thousand genome-wide sequence markers and use these to analyze patterns of population divergence across two pairs of parallel hybrid zones in Peru and Ecuador. We compare two approaches for analysis of this type of data—alignment to a reference genome and de novo assembly—and find that alignment gives the best results for species both closely (H. melpomene) and distantly (H. erato, ∼15% divergent) related to the reference sequence. Our results confirm that the color pattern controlling loci account for the majority of divergent regions across the genome, but we also detect other divergent regions apparently unlinked to color pattern differences. We also use association mapping to identify previously unmapped color pattern loci, in particular the Ro locus. Finally, we identify a new cryptic population of H. timareta in Ecuador, which occurs at relatively low altitude and is mimetic with H. melpomene malleti. |
format | Online Article Text |
id | pubmed-4120085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41200852015-02-01 Population genomics of parallel hybrid zones in the mimetic butterflies, H. melpomene and H. erato Nadeau, Nicola J. Ruiz, Mayté Salazar, Patricio Counterman, Brian Medina, Jose Alejandro Ortiz-Zuazaga, Humberto Morrison, Anna McMillan, W. Owen Jiggins, Chris D. Papa, Riccardo Genome Res Research Hybrid zones can be valuable tools for studying evolution and identifying genomic regions responsible for adaptive divergence and underlying phenotypic variation. Hybrid zones between subspecies of Heliconius butterflies can be very narrow and are maintained by strong selection acting on color pattern. The comimetic species, H. erato and H. melpomene, have parallel hybrid zones in which both species undergo a change from one color pattern form to another. We use restriction-associated DNA sequencing to obtain several thousand genome-wide sequence markers and use these to analyze patterns of population divergence across two pairs of parallel hybrid zones in Peru and Ecuador. We compare two approaches for analysis of this type of data—alignment to a reference genome and de novo assembly—and find that alignment gives the best results for species both closely (H. melpomene) and distantly (H. erato, ∼15% divergent) related to the reference sequence. Our results confirm that the color pattern controlling loci account for the majority of divergent regions across the genome, but we also detect other divergent regions apparently unlinked to color pattern differences. We also use association mapping to identify previously unmapped color pattern loci, in particular the Ro locus. Finally, we identify a new cryptic population of H. timareta in Ecuador, which occurs at relatively low altitude and is mimetic with H. melpomene malleti. Cold Spring Harbor Laboratory Press 2014-08 /pmc/articles/PMC4120085/ /pubmed/24823669 http://dx.doi.org/10.1101/gr.169292.113 Text en © 2014 Nadeau et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Nadeau, Nicola J. Ruiz, Mayté Salazar, Patricio Counterman, Brian Medina, Jose Alejandro Ortiz-Zuazaga, Humberto Morrison, Anna McMillan, W. Owen Jiggins, Chris D. Papa, Riccardo Population genomics of parallel hybrid zones in the mimetic butterflies, H. melpomene and H. erato |
title | Population genomics of parallel hybrid zones in the mimetic butterflies, H. melpomene and H. erato |
title_full | Population genomics of parallel hybrid zones in the mimetic butterflies, H. melpomene and H. erato |
title_fullStr | Population genomics of parallel hybrid zones in the mimetic butterflies, H. melpomene and H. erato |
title_full_unstemmed | Population genomics of parallel hybrid zones in the mimetic butterflies, H. melpomene and H. erato |
title_short | Population genomics of parallel hybrid zones in the mimetic butterflies, H. melpomene and H. erato |
title_sort | population genomics of parallel hybrid zones in the mimetic butterflies, h. melpomene and h. erato |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120085/ https://www.ncbi.nlm.nih.gov/pubmed/24823669 http://dx.doi.org/10.1101/gr.169292.113 |
work_keys_str_mv | AT nadeaunicolaj populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato AT ruizmayte populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato AT salazarpatricio populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato AT countermanbrian populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato AT medinajosealejandro populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato AT ortizzuazagahumberto populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato AT morrisonanna populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato AT mcmillanwowen populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato AT jigginschrisd populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato AT papariccardo populationgenomicsofparallelhybridzonesinthemimeticbutterflieshmelpomeneandherato |