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EphrinB2 Affects Apical Constriction in Xenopus Embryos and is Regulated by ADAM10 and Flotillin-1
The Eph/ephrin signaling pathways have a critical function in cell adhesion and repulsion, and thus play key roles in various morphogenetic events during development. Here we show that a decrease in ephrinB2 protein causes neural tube closure defects during Xenopus laevis embryogenesis. Such a decre...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120273/ https://www.ncbi.nlm.nih.gov/pubmed/24662724 http://dx.doi.org/10.1038/ncomms4516 |
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author | Ji, Yon Ju Hwang, Yoo-Seok Mood, Kathleen Cho, Hee-Jun Lee, Hyun-Shik Winterbottom, Emily Cousin, Hèléne Daar, Ira O. |
author_facet | Ji, Yon Ju Hwang, Yoo-Seok Mood, Kathleen Cho, Hee-Jun Lee, Hyun-Shik Winterbottom, Emily Cousin, Hèléne Daar, Ira O. |
author_sort | Ji, Yon Ju |
collection | PubMed |
description | The Eph/ephrin signaling pathways have a critical function in cell adhesion and repulsion, and thus play key roles in various morphogenetic events during development. Here we show that a decrease in ephrinB2 protein causes neural tube closure defects during Xenopus laevis embryogenesis. Such a decrease in ephrinB2 protein levels is observed upon the loss of flotillin-1 scaffold protein, a newly identified ephrinB2-binding partner. This dramatic decline in ephrinB2 protein levels upon the absence of flotillin-1 expression is specific, and is partly the result of an increased susceptibility to cleavage by the metalloprotease ADAM10. These findings indicate that flotillin-1 regulates ephrinB2 protein levels through ADAM10, and is required for appropriate neural tube morphogenesis in the Xenopus embryo. |
format | Online Article Text |
id | pubmed-4120273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-41202732014-09-24 EphrinB2 Affects Apical Constriction in Xenopus Embryos and is Regulated by ADAM10 and Flotillin-1 Ji, Yon Ju Hwang, Yoo-Seok Mood, Kathleen Cho, Hee-Jun Lee, Hyun-Shik Winterbottom, Emily Cousin, Hèléne Daar, Ira O. Nat Commun Article The Eph/ephrin signaling pathways have a critical function in cell adhesion and repulsion, and thus play key roles in various morphogenetic events during development. Here we show that a decrease in ephrinB2 protein causes neural tube closure defects during Xenopus laevis embryogenesis. Such a decrease in ephrinB2 protein levels is observed upon the loss of flotillin-1 scaffold protein, a newly identified ephrinB2-binding partner. This dramatic decline in ephrinB2 protein levels upon the absence of flotillin-1 expression is specific, and is partly the result of an increased susceptibility to cleavage by the metalloprotease ADAM10. These findings indicate that flotillin-1 regulates ephrinB2 protein levels through ADAM10, and is required for appropriate neural tube morphogenesis in the Xenopus embryo. 2014-03-24 /pmc/articles/PMC4120273/ /pubmed/24662724 http://dx.doi.org/10.1038/ncomms4516 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Ji, Yon Ju Hwang, Yoo-Seok Mood, Kathleen Cho, Hee-Jun Lee, Hyun-Shik Winterbottom, Emily Cousin, Hèléne Daar, Ira O. EphrinB2 Affects Apical Constriction in Xenopus Embryos and is Regulated by ADAM10 and Flotillin-1 |
title | EphrinB2 Affects Apical Constriction in Xenopus Embryos and is Regulated by ADAM10 and Flotillin-1 |
title_full | EphrinB2 Affects Apical Constriction in Xenopus Embryos and is Regulated by ADAM10 and Flotillin-1 |
title_fullStr | EphrinB2 Affects Apical Constriction in Xenopus Embryos and is Regulated by ADAM10 and Flotillin-1 |
title_full_unstemmed | EphrinB2 Affects Apical Constriction in Xenopus Embryos and is Regulated by ADAM10 and Flotillin-1 |
title_short | EphrinB2 Affects Apical Constriction in Xenopus Embryos and is Regulated by ADAM10 and Flotillin-1 |
title_sort | ephrinb2 affects apical constriction in xenopus embryos and is regulated by adam10 and flotillin-1 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120273/ https://www.ncbi.nlm.nih.gov/pubmed/24662724 http://dx.doi.org/10.1038/ncomms4516 |
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