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Xilei San Ameliorates Experimental Colitis in Rats by Selectively Degrading Proinflammatory Mediators and Promoting Mucosal Repair
Xilei san (XLS), a herbal preparation widely used in China for erosive and ulcerative diseases, has been shown to be effective in ulcerative colitis (UC). The present experiments were conducted to assess its efficacy and determine its mechanism of action in a rat model that resembles human UC. The m...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120479/ https://www.ncbi.nlm.nih.gov/pubmed/25120575 http://dx.doi.org/10.1155/2014/569587 |
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author | Hao, Yongbiao Nagase, Kazuko Hori, Kazutoshi Wang, Shenglan Kogure, Yoko Fukunaga, Ken Kashiwamura, Shinichiro Yamamoto, Satoshi Nakamura, Shiro Li, Junxiang Miwa, Hiroto Noguchi, Koichi Dai, Yi |
author_facet | Hao, Yongbiao Nagase, Kazuko Hori, Kazutoshi Wang, Shenglan Kogure, Yoko Fukunaga, Ken Kashiwamura, Shinichiro Yamamoto, Satoshi Nakamura, Shiro Li, Junxiang Miwa, Hiroto Noguchi, Koichi Dai, Yi |
author_sort | Hao, Yongbiao |
collection | PubMed |
description | Xilei san (XLS), a herbal preparation widely used in China for erosive and ulcerative diseases, has been shown to be effective in ulcerative colitis (UC). The present experiments were conducted to assess its efficacy and determine its mechanism of action in a rat model that resembles human UC. The model was induced by adding 4% dextran sulfate sodium (DSS) to the rats' drinking water for 7 days. XLS was administered daily by retention enema from day 2 to day 7; the rats were sacrificed on day 8. The colon tissues were obtained for further experiments. A histological damage score and the activity of tissue myeloperoxidase were used to evaluate the severity of the colitis. The colonic cytokine levels were detected in a suspension array, and epithelial proliferation was assessed using Ki-67 immunohistochemistry. Intrarectal administration of XLS attenuated the DSS-induced colitis, as evidenced by a reduction in both the histological damage score and myeloperoxidase activity. It also decreased the levels of proinflammatory cytokines, but increased the mucosal repair-related cytokines. In addition, the epithelial Ki-67 expression was upregulated by XLS. These results suggest that XLS attenuates DSS-induced colitis by degrading proinflammatory mediators and promoting mucosal repair. XLS could be a potential topical treatment for human UC. |
format | Online Article Text |
id | pubmed-4120479 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41204792014-08-12 Xilei San Ameliorates Experimental Colitis in Rats by Selectively Degrading Proinflammatory Mediators and Promoting Mucosal Repair Hao, Yongbiao Nagase, Kazuko Hori, Kazutoshi Wang, Shenglan Kogure, Yoko Fukunaga, Ken Kashiwamura, Shinichiro Yamamoto, Satoshi Nakamura, Shiro Li, Junxiang Miwa, Hiroto Noguchi, Koichi Dai, Yi Evid Based Complement Alternat Med Research Article Xilei san (XLS), a herbal preparation widely used in China for erosive and ulcerative diseases, has been shown to be effective in ulcerative colitis (UC). The present experiments were conducted to assess its efficacy and determine its mechanism of action in a rat model that resembles human UC. The model was induced by adding 4% dextran sulfate sodium (DSS) to the rats' drinking water for 7 days. XLS was administered daily by retention enema from day 2 to day 7; the rats were sacrificed on day 8. The colon tissues were obtained for further experiments. A histological damage score and the activity of tissue myeloperoxidase were used to evaluate the severity of the colitis. The colonic cytokine levels were detected in a suspension array, and epithelial proliferation was assessed using Ki-67 immunohistochemistry. Intrarectal administration of XLS attenuated the DSS-induced colitis, as evidenced by a reduction in both the histological damage score and myeloperoxidase activity. It also decreased the levels of proinflammatory cytokines, but increased the mucosal repair-related cytokines. In addition, the epithelial Ki-67 expression was upregulated by XLS. These results suggest that XLS attenuates DSS-induced colitis by degrading proinflammatory mediators and promoting mucosal repair. XLS could be a potential topical treatment for human UC. Hindawi Publishing Corporation 2014 2014-07-10 /pmc/articles/PMC4120479/ /pubmed/25120575 http://dx.doi.org/10.1155/2014/569587 Text en Copyright © 2014 Yongbiao Hao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hao, Yongbiao Nagase, Kazuko Hori, Kazutoshi Wang, Shenglan Kogure, Yoko Fukunaga, Ken Kashiwamura, Shinichiro Yamamoto, Satoshi Nakamura, Shiro Li, Junxiang Miwa, Hiroto Noguchi, Koichi Dai, Yi Xilei San Ameliorates Experimental Colitis in Rats by Selectively Degrading Proinflammatory Mediators and Promoting Mucosal Repair |
title | Xilei San Ameliorates Experimental Colitis in Rats by Selectively Degrading Proinflammatory Mediators and Promoting Mucosal Repair |
title_full | Xilei San Ameliorates Experimental Colitis in Rats by Selectively Degrading Proinflammatory Mediators and Promoting Mucosal Repair |
title_fullStr | Xilei San Ameliorates Experimental Colitis in Rats by Selectively Degrading Proinflammatory Mediators and Promoting Mucosal Repair |
title_full_unstemmed | Xilei San Ameliorates Experimental Colitis in Rats by Selectively Degrading Proinflammatory Mediators and Promoting Mucosal Repair |
title_short | Xilei San Ameliorates Experimental Colitis in Rats by Selectively Degrading Proinflammatory Mediators and Promoting Mucosal Repair |
title_sort | xilei san ameliorates experimental colitis in rats by selectively degrading proinflammatory mediators and promoting mucosal repair |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120479/ https://www.ncbi.nlm.nih.gov/pubmed/25120575 http://dx.doi.org/10.1155/2014/569587 |
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