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Curvelet Based Offline Analysis of SEM Images

Manual offline analysis, of a scanning electron microscopy (SEM) image, is a time consuming process and requires continuous human intervention and efforts. This paper presents an image processing based method for automated offline analyses of SEM images. To this end, our strategy relies on a two-sta...

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Detalles Bibliográficos
Autores principales: Shirazi, Syed Hamad, Haq, Nuhman ul, Hayat, Khizar, Naz, Saeeda, Haque, Ihsan ul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121203/
https://www.ncbi.nlm.nih.gov/pubmed/25089617
http://dx.doi.org/10.1371/journal.pone.0103942
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author Shirazi, Syed Hamad
Haq, Nuhman ul
Hayat, Khizar
Naz, Saeeda
Haque, Ihsan ul
author_facet Shirazi, Syed Hamad
Haq, Nuhman ul
Hayat, Khizar
Naz, Saeeda
Haque, Ihsan ul
author_sort Shirazi, Syed Hamad
collection PubMed
description Manual offline analysis, of a scanning electron microscopy (SEM) image, is a time consuming process and requires continuous human intervention and efforts. This paper presents an image processing based method for automated offline analyses of SEM images. To this end, our strategy relies on a two-stage process, viz. texture analysis and quantification. The method involves a preprocessing step, aimed at the noise removal, in order to avoid false edges. For texture analysis, the proposed method employs a state of the art Curvelet transform followed by segmentation through a combination of entropy filtering, thresholding and mathematical morphology (MM). The quantification is carried out by the application of a box-counting algorithm, for fractal dimension (FD) calculations, with the ultimate goal of measuring the parameters, like surface area and perimeter. The perimeter is estimated indirectly by counting the boundary boxes of the filled shapes. The proposed method, when applied to a representative set of SEM images, not only showed better results in image segmentation but also exhibited a good accuracy in the calculation of surface area and perimeter. The proposed method outperforms the well-known Watershed segmentation algorithm.
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spelling pubmed-41212032014-08-05 Curvelet Based Offline Analysis of SEM Images Shirazi, Syed Hamad Haq, Nuhman ul Hayat, Khizar Naz, Saeeda Haque, Ihsan ul PLoS One Research Article Manual offline analysis, of a scanning electron microscopy (SEM) image, is a time consuming process and requires continuous human intervention and efforts. This paper presents an image processing based method for automated offline analyses of SEM images. To this end, our strategy relies on a two-stage process, viz. texture analysis and quantification. The method involves a preprocessing step, aimed at the noise removal, in order to avoid false edges. For texture analysis, the proposed method employs a state of the art Curvelet transform followed by segmentation through a combination of entropy filtering, thresholding and mathematical morphology (MM). The quantification is carried out by the application of a box-counting algorithm, for fractal dimension (FD) calculations, with the ultimate goal of measuring the parameters, like surface area and perimeter. The perimeter is estimated indirectly by counting the boundary boxes of the filled shapes. The proposed method, when applied to a representative set of SEM images, not only showed better results in image segmentation but also exhibited a good accuracy in the calculation of surface area and perimeter. The proposed method outperforms the well-known Watershed segmentation algorithm. Public Library of Science 2014-08-04 /pmc/articles/PMC4121203/ /pubmed/25089617 http://dx.doi.org/10.1371/journal.pone.0103942 Text en © 2014 Shirazi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shirazi, Syed Hamad
Haq, Nuhman ul
Hayat, Khizar
Naz, Saeeda
Haque, Ihsan ul
Curvelet Based Offline Analysis of SEM Images
title Curvelet Based Offline Analysis of SEM Images
title_full Curvelet Based Offline Analysis of SEM Images
title_fullStr Curvelet Based Offline Analysis of SEM Images
title_full_unstemmed Curvelet Based Offline Analysis of SEM Images
title_short Curvelet Based Offline Analysis of SEM Images
title_sort curvelet based offline analysis of sem images
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121203/
https://www.ncbi.nlm.nih.gov/pubmed/25089617
http://dx.doi.org/10.1371/journal.pone.0103942
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