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Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria
Curcuma zedoaria also known as Temu putih is traditionally used in food preparations and treatment of various ailments including cancer. The cytotoxic activity of hexane, dichloromethane, ethyl acetate, methanol, and the methanol-soxhlet extracts of Curcuma zedoaria rhizomes was tested on two human...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121215/ https://www.ncbi.nlm.nih.gov/pubmed/25126594 http://dx.doi.org/10.1155/2014/321943 |
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author | Ahmed Hamdi, Omer Abdalla Syed Abdul Rahman, Syarifah Nur Awang, Khalijah Abdul Wahab, Norhanom Looi, Chung Yeng Thomas, Noel Francis Abd Malek, Sri Nurestri |
author_facet | Ahmed Hamdi, Omer Abdalla Syed Abdul Rahman, Syarifah Nur Awang, Khalijah Abdul Wahab, Norhanom Looi, Chung Yeng Thomas, Noel Francis Abd Malek, Sri Nurestri |
author_sort | Ahmed Hamdi, Omer Abdalla |
collection | PubMed |
description | Curcuma zedoaria also known as Temu putih is traditionally used in food preparations and treatment of various ailments including cancer. The cytotoxic activity of hexane, dichloromethane, ethyl acetate, methanol, and the methanol-soxhlet extracts of Curcuma zedoaria rhizomes was tested on two human cancer cell lines (Ca Ski and MCF-7) and a noncancer cell line (HUVEC) using MTT assay. Investigation on the chemical components in the hexane and dichloromethane fractions gave 19 compounds, namely, labda-8(17),12 diene-15,16 dial (1), dehydrocurdione (2), curcumenone (3), comosone II (4), curcumenol (5), procurcumenol (6), germacrone (7), zerumbone epoxide (8), zederone (9), 9-isopropylidene-2,6-dimethyl-11-oxatricyclo[6.2.1.0(1,5)]undec-6-en-8-ol (10), furanodiene (11), germacrone-4,5-epoxide (12), calcaratarin A (13), isoprocurcumenol (14), germacrone-1,10-epoxide (15), zerumin A (16), curcumanolide A (17), curcuzedoalide (18), and gweicurculactone (19). Compounds (1–19) were evaluated for their antiproliferative effect using MTT assay against four cancer cell lines (Ca Ski, MCF-7, PC-3, and HT-29). Curcumenone (3) and curcumenol (5) displayed strong antiproliferative activity (IC(50) = 8.3 ± 1.0 and 9.3 ± 0.3 μg/mL, resp.) and were found to induce apoptotic cell death on MCF-7 cells using phase contrast and Hoechst 33342/PI double-staining assay. Thus, the present study provides basis for the ethnomedical application of Curcuma zedoaria in the treatment of breast cancer. |
format | Online Article Text |
id | pubmed-4121215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41212152014-08-14 Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria Ahmed Hamdi, Omer Abdalla Syed Abdul Rahman, Syarifah Nur Awang, Khalijah Abdul Wahab, Norhanom Looi, Chung Yeng Thomas, Noel Francis Abd Malek, Sri Nurestri ScientificWorldJournal Research Article Curcuma zedoaria also known as Temu putih is traditionally used in food preparations and treatment of various ailments including cancer. The cytotoxic activity of hexane, dichloromethane, ethyl acetate, methanol, and the methanol-soxhlet extracts of Curcuma zedoaria rhizomes was tested on two human cancer cell lines (Ca Ski and MCF-7) and a noncancer cell line (HUVEC) using MTT assay. Investigation on the chemical components in the hexane and dichloromethane fractions gave 19 compounds, namely, labda-8(17),12 diene-15,16 dial (1), dehydrocurdione (2), curcumenone (3), comosone II (4), curcumenol (5), procurcumenol (6), germacrone (7), zerumbone epoxide (8), zederone (9), 9-isopropylidene-2,6-dimethyl-11-oxatricyclo[6.2.1.0(1,5)]undec-6-en-8-ol (10), furanodiene (11), germacrone-4,5-epoxide (12), calcaratarin A (13), isoprocurcumenol (14), germacrone-1,10-epoxide (15), zerumin A (16), curcumanolide A (17), curcuzedoalide (18), and gweicurculactone (19). Compounds (1–19) were evaluated for their antiproliferative effect using MTT assay against four cancer cell lines (Ca Ski, MCF-7, PC-3, and HT-29). Curcumenone (3) and curcumenol (5) displayed strong antiproliferative activity (IC(50) = 8.3 ± 1.0 and 9.3 ± 0.3 μg/mL, resp.) and were found to induce apoptotic cell death on MCF-7 cells using phase contrast and Hoechst 33342/PI double-staining assay. Thus, the present study provides basis for the ethnomedical application of Curcuma zedoaria in the treatment of breast cancer. Hindawi Publishing Corporation 2014 2014-07-13 /pmc/articles/PMC4121215/ /pubmed/25126594 http://dx.doi.org/10.1155/2014/321943 Text en Copyright © 2014 Omer Abdalla Ahmed Hamdi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ahmed Hamdi, Omer Abdalla Syed Abdul Rahman, Syarifah Nur Awang, Khalijah Abdul Wahab, Norhanom Looi, Chung Yeng Thomas, Noel Francis Abd Malek, Sri Nurestri Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria |
title | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria
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title_full | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria
|
title_fullStr | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria
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title_full_unstemmed | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria
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title_short | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria
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title_sort | cytotoxic constituents from the rhizomes of curcuma zedoaria |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121215/ https://www.ncbi.nlm.nih.gov/pubmed/25126594 http://dx.doi.org/10.1155/2014/321943 |
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