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Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation

High mobility group box 1 (HMGB1), a nonhistone DNA-binding protein, is released into the extracellular space and promotes inflammation. HMGB1 binds to related cell signaling transduction receptors, including receptor for advanced glycation end products (RAGE), which actively participate in vascular...

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Autores principales: Tancharoen, Salunya, Tengrungsun, Tassanee, Suddhasthira, Theeralaksna, Kikuchi, Kiyoshi, Vechvongvan, Nuttavun, Tokuda, Masayuki, Maruyama, Ikuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121219/
https://www.ncbi.nlm.nih.gov/pubmed/25114379
http://dx.doi.org/10.1155/2014/754069
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author Tancharoen, Salunya
Tengrungsun, Tassanee
Suddhasthira, Theeralaksna
Kikuchi, Kiyoshi
Vechvongvan, Nuttavun
Tokuda, Masayuki
Maruyama, Ikuro
author_facet Tancharoen, Salunya
Tengrungsun, Tassanee
Suddhasthira, Theeralaksna
Kikuchi, Kiyoshi
Vechvongvan, Nuttavun
Tokuda, Masayuki
Maruyama, Ikuro
author_sort Tancharoen, Salunya
collection PubMed
description High mobility group box 1 (HMGB1), a nonhistone DNA-binding protein, is released into the extracellular space and promotes inflammation. HMGB1 binds to related cell signaling transduction receptors, including receptor for advanced glycation end products (RAGE), which actively participate in vascular and inflammatory diseases. The aim of this study was to examine whether RAGE and HMGB1 are involved in the pathogenesis of pulpitis and investigate the effect of Prevotella intermedia (P. intermedia) lipopolysaccharide (LPS) on RAGE and HMGB1 expression in odontoblast-like cells (OLC-1). RAGE and HMGB1 expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. Upregulated expression of RAGE was observed in odontoblasts, stromal pulp fibroblasts-like cells, and endothelial-like cell lining human pulpitis tissue. Strong cytoplasmic HMGB1 immunoreactivity was noted in odontoblasts, whereas nuclear HMGB1 immunoreactivity was seen in stromal pulp fibroblasts-like cells in human pulpitis tissue. LPS stimulated OLC-1 cells produced HMGB1 in a dose-dependent manner through RAGE. HMGB1 translocation towards the cytoplasm and secretion from OLC-1 in response to LPS was inhibited by TPCA-1, an inhibitor of NF-κB activation. These findings suggest that RAGE and HMGB1 play an important role in the pulpal immune response to oral bacterial infection.
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spelling pubmed-41212192014-08-11 Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation Tancharoen, Salunya Tengrungsun, Tassanee Suddhasthira, Theeralaksna Kikuchi, Kiyoshi Vechvongvan, Nuttavun Tokuda, Masayuki Maruyama, Ikuro Mediators Inflamm Research Article High mobility group box 1 (HMGB1), a nonhistone DNA-binding protein, is released into the extracellular space and promotes inflammation. HMGB1 binds to related cell signaling transduction receptors, including receptor for advanced glycation end products (RAGE), which actively participate in vascular and inflammatory diseases. The aim of this study was to examine whether RAGE and HMGB1 are involved in the pathogenesis of pulpitis and investigate the effect of Prevotella intermedia (P. intermedia) lipopolysaccharide (LPS) on RAGE and HMGB1 expression in odontoblast-like cells (OLC-1). RAGE and HMGB1 expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. Upregulated expression of RAGE was observed in odontoblasts, stromal pulp fibroblasts-like cells, and endothelial-like cell lining human pulpitis tissue. Strong cytoplasmic HMGB1 immunoreactivity was noted in odontoblasts, whereas nuclear HMGB1 immunoreactivity was seen in stromal pulp fibroblasts-like cells in human pulpitis tissue. LPS stimulated OLC-1 cells produced HMGB1 in a dose-dependent manner through RAGE. HMGB1 translocation towards the cytoplasm and secretion from OLC-1 in response to LPS was inhibited by TPCA-1, an inhibitor of NF-κB activation. These findings suggest that RAGE and HMGB1 play an important role in the pulpal immune response to oral bacterial infection. Hindawi Publishing Corporation 2014 2014-07-10 /pmc/articles/PMC4121219/ /pubmed/25114379 http://dx.doi.org/10.1155/2014/754069 Text en Copyright © 2014 Salunya Tancharoen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tancharoen, Salunya
Tengrungsun, Tassanee
Suddhasthira, Theeralaksna
Kikuchi, Kiyoshi
Vechvongvan, Nuttavun
Tokuda, Masayuki
Maruyama, Ikuro
Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation
title Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation
title_full Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation
title_fullStr Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation
title_full_unstemmed Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation
title_short Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation
title_sort overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121219/
https://www.ncbi.nlm.nih.gov/pubmed/25114379
http://dx.doi.org/10.1155/2014/754069
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