Cargando…
High Resolution Scanning Electron Microscopy of Cells Using Dielectrophoresis
Ultrastructural analysis of cells can reveal valuable information about their morphological, physiological, and biochemical characteristics. Scanning electron microscopy (SEM) has been widely used to provide high-resolution images from the surface of biological samples. However, samples need to be d...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121316/ https://www.ncbi.nlm.nih.gov/pubmed/25089528 http://dx.doi.org/10.1371/journal.pone.0104109 |
_version_ | 1782329216389349376 |
---|---|
author | Tang, Shi-Yang Zhang, Wei Soffe, Rebecca Nahavandi, Sofia Shukla, Ravi Khoshmanesh, Khashayar |
author_facet | Tang, Shi-Yang Zhang, Wei Soffe, Rebecca Nahavandi, Sofia Shukla, Ravi Khoshmanesh, Khashayar |
author_sort | Tang, Shi-Yang |
collection | PubMed |
description | Ultrastructural analysis of cells can reveal valuable information about their morphological, physiological, and biochemical characteristics. Scanning electron microscopy (SEM) has been widely used to provide high-resolution images from the surface of biological samples. However, samples need to be dehydrated and coated with conductive materials for SEM imaging. Besides, immobilizing non-adherent cells during processing and analysis is challenging and requires complex fixation protocols. In this work, we developed a novel dielectrophoresis based microfluidic platform for interfacing non-adherent cells with high-resolution SEM at low vacuum mode. The system enables rapid immobilization and dehydration of samples without deposition of chemical residues over the cell surface. Moreover, it enables the on-chip chemical stimulation and fixation of immobilized cells with minimum dislodgement. These advantages were demonstrated for comparing the morphological changes of non-budding and budding yeast cells following Lyticase treatment. |
format | Online Article Text |
id | pubmed-4121316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41213162014-08-05 High Resolution Scanning Electron Microscopy of Cells Using Dielectrophoresis Tang, Shi-Yang Zhang, Wei Soffe, Rebecca Nahavandi, Sofia Shukla, Ravi Khoshmanesh, Khashayar PLoS One Research Article Ultrastructural analysis of cells can reveal valuable information about their morphological, physiological, and biochemical characteristics. Scanning electron microscopy (SEM) has been widely used to provide high-resolution images from the surface of biological samples. However, samples need to be dehydrated and coated with conductive materials for SEM imaging. Besides, immobilizing non-adherent cells during processing and analysis is challenging and requires complex fixation protocols. In this work, we developed a novel dielectrophoresis based microfluidic platform for interfacing non-adherent cells with high-resolution SEM at low vacuum mode. The system enables rapid immobilization and dehydration of samples without deposition of chemical residues over the cell surface. Moreover, it enables the on-chip chemical stimulation and fixation of immobilized cells with minimum dislodgement. These advantages were demonstrated for comparing the morphological changes of non-budding and budding yeast cells following Lyticase treatment. Public Library of Science 2014-08-04 /pmc/articles/PMC4121316/ /pubmed/25089528 http://dx.doi.org/10.1371/journal.pone.0104109 Text en © 2014 Tang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tang, Shi-Yang Zhang, Wei Soffe, Rebecca Nahavandi, Sofia Shukla, Ravi Khoshmanesh, Khashayar High Resolution Scanning Electron Microscopy of Cells Using Dielectrophoresis |
title | High Resolution Scanning Electron Microscopy of Cells Using Dielectrophoresis |
title_full | High Resolution Scanning Electron Microscopy of Cells Using Dielectrophoresis |
title_fullStr | High Resolution Scanning Electron Microscopy of Cells Using Dielectrophoresis |
title_full_unstemmed | High Resolution Scanning Electron Microscopy of Cells Using Dielectrophoresis |
title_short | High Resolution Scanning Electron Microscopy of Cells Using Dielectrophoresis |
title_sort | high resolution scanning electron microscopy of cells using dielectrophoresis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121316/ https://www.ncbi.nlm.nih.gov/pubmed/25089528 http://dx.doi.org/10.1371/journal.pone.0104109 |
work_keys_str_mv | AT tangshiyang highresolutionscanningelectronmicroscopyofcellsusingdielectrophoresis AT zhangwei highresolutionscanningelectronmicroscopyofcellsusingdielectrophoresis AT sofferebecca highresolutionscanningelectronmicroscopyofcellsusingdielectrophoresis AT nahavandisofia highresolutionscanningelectronmicroscopyofcellsusingdielectrophoresis AT shuklaravi highresolutionscanningelectronmicroscopyofcellsusingdielectrophoresis AT khoshmaneshkhashayar highresolutionscanningelectronmicroscopyofcellsusingdielectrophoresis |