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Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

Octamer-binding transcription factor 4 (OCT4) is one of the factors associated with self-renewal and differentiation in cancer stem cells, and is crucial for the progression of various types of human malignancy. However, the expression and function of OCT4 in human pancreatic cancer has not been ful...

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Autores principales: LIN, HAI, SUN, LI-HUA, HAN, WEI, HE, TIE-YING, XU, XIN-JIAN, CHENG, KUN, GENG, CHENG, SU, LI-DAN, WEN, HAO, WANG, XI-YAN, CHEN, QI-LONG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121418/
https://www.ncbi.nlm.nih.gov/pubmed/25017645
http://dx.doi.org/10.3892/mmr.2014.2367
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author LIN, HAI
SUN, LI-HUA
HAN, WEI
HE, TIE-YING
XU, XIN-JIAN
CHENG, KUN
GENG, CHENG
SU, LI-DAN
WEN, HAO
WANG, XI-YAN
CHEN, QI-LONG
author_facet LIN, HAI
SUN, LI-HUA
HAN, WEI
HE, TIE-YING
XU, XIN-JIAN
CHENG, KUN
GENG, CHENG
SU, LI-DAN
WEN, HAO
WANG, XI-YAN
CHEN, QI-LONG
author_sort LIN, HAI
collection PubMed
description Octamer-binding transcription factor 4 (OCT4) is one of the factors associated with self-renewal and differentiation in cancer stem cells, and is crucial for the progression of various types of human malignancy. However, the expression and function of OCT4 in human pancreatic cancer has not been fully elucidated. The purpose of the present study was to investigate the function and molecular mechanisms of OCT4 in pancreatic cancer cells. The clinical significance of OCT4 expression was assessed by an immunohistochemical assay using a tissue microarray procedure in pancreatic cancer tissues and cells with different degrees of differentiation. A loss-of-function approach was used to examine the effects of a lentivirus-mediated OCT4 small hairpin RNA vector on biological behaviors, including cell proliferative activity and invasive potential. The results demonstrated that the expression levels of OCT4 protein in cancer tissues were significantly elevated compared with those in adjacent non-cancerous tissues (65.0 vs. 42.5%; P=0.005), which was correlated with tumor differentiation (P=0.008). The knockdown of OCT4 inhibited the proliferation and invasion of pancreatic cancer cells (Panc-1) expressing high levels of OCT4, accompanied with decreased expression of AKT, proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase-2 (MMP-2). In conclusion, the present study reveals that the increased expression of OCT4 is correlated with the differentiation of pancreatic cancer, while knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of AKT pathway-mediated PCNA and MMP-2 expression, suggesting that OCT4 might serve as a potential therapeutic target for the treatment of pancreatic cancer.
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spelling pubmed-41214182014-08-14 Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway LIN, HAI SUN, LI-HUA HAN, WEI HE, TIE-YING XU, XIN-JIAN CHENG, KUN GENG, CHENG SU, LI-DAN WEN, HAO WANG, XI-YAN CHEN, QI-LONG Mol Med Rep Articles Octamer-binding transcription factor 4 (OCT4) is one of the factors associated with self-renewal and differentiation in cancer stem cells, and is crucial for the progression of various types of human malignancy. However, the expression and function of OCT4 in human pancreatic cancer has not been fully elucidated. The purpose of the present study was to investigate the function and molecular mechanisms of OCT4 in pancreatic cancer cells. The clinical significance of OCT4 expression was assessed by an immunohistochemical assay using a tissue microarray procedure in pancreatic cancer tissues and cells with different degrees of differentiation. A loss-of-function approach was used to examine the effects of a lentivirus-mediated OCT4 small hairpin RNA vector on biological behaviors, including cell proliferative activity and invasive potential. The results demonstrated that the expression levels of OCT4 protein in cancer tissues were significantly elevated compared with those in adjacent non-cancerous tissues (65.0 vs. 42.5%; P=0.005), which was correlated with tumor differentiation (P=0.008). The knockdown of OCT4 inhibited the proliferation and invasion of pancreatic cancer cells (Panc-1) expressing high levels of OCT4, accompanied with decreased expression of AKT, proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase-2 (MMP-2). In conclusion, the present study reveals that the increased expression of OCT4 is correlated with the differentiation of pancreatic cancer, while knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of AKT pathway-mediated PCNA and MMP-2 expression, suggesting that OCT4 might serve as a potential therapeutic target for the treatment of pancreatic cancer. D.A. Spandidos 2014-09 2014-07-07 /pmc/articles/PMC4121418/ /pubmed/25017645 http://dx.doi.org/10.3892/mmr.2014.2367 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
LIN, HAI
SUN, LI-HUA
HAN, WEI
HE, TIE-YING
XU, XIN-JIAN
CHENG, KUN
GENG, CHENG
SU, LI-DAN
WEN, HAO
WANG, XI-YAN
CHEN, QI-LONG
Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway
title Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway
title_full Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway
title_fullStr Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway
title_full_unstemmed Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway
title_short Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway
title_sort knockdown of oct4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the akt pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121418/
https://www.ncbi.nlm.nih.gov/pubmed/25017645
http://dx.doi.org/10.3892/mmr.2014.2367
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