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Circulating Muscle-specific miRNAs in Duchenne Muscular Dystrophy Patients

Noninvasive biomarkers with diagnostic value and prognostic applications have long been desired to replace muscle biopsy for Duchenne muscular dystrophy (DMD) patients. Growing evidence indicates that circulating microRNAs are biomarkers to assess pathophysiological status. Here, we show that the se...

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Autores principales: Li, Xihua, Li, Yuying, Zhao, Lei, Zhang, Duo, Yao, Xuan, Zhang, Huihui, Wang, Yu-cheng, Wang, Xin-yi, Xia, Hongfeng, Yan, Jun, Ying, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121518/
https://www.ncbi.nlm.nih.gov/pubmed/25050825
http://dx.doi.org/10.1038/mtna.2014.29
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author Li, Xihua
Li, Yuying
Zhao, Lei
Zhang, Duo
Yao, Xuan
Zhang, Huihui
Wang, Yu-cheng
Wang, Xin-yi
Xia, Hongfeng
Yan, Jun
Ying, Hao
author_facet Li, Xihua
Li, Yuying
Zhao, Lei
Zhang, Duo
Yao, Xuan
Zhang, Huihui
Wang, Yu-cheng
Wang, Xin-yi
Xia, Hongfeng
Yan, Jun
Ying, Hao
author_sort Li, Xihua
collection PubMed
description Noninvasive biomarkers with diagnostic value and prognostic applications have long been desired to replace muscle biopsy for Duchenne muscular dystrophy (DMD) patients. Growing evidence indicates that circulating microRNAs are biomarkers to assess pathophysiological status. Here, we show that the serum levels of six muscle-specific miRNAs (miR-1/206/133/499/208a/208b, also known as myomiRs) were all elevated in DMD patients (P < 0.01). The receiver operating characteristic curves of circulating miR-206, miR-499, miR-208b, and miR-133 levels reflected strong separation between Becker's muscular dystrophy (BMD) and DMD patients (P < 0.05). miR-206, miR-499, and miR-208b levels were positively correlated with both age and type IIc muscle fiber content in DMD patients (2–6 years), indicating that they might represent the stage of disease as well as the process of regeneration. miR-499 and miR-208b levels were correlated with slow and fast fiber content and might reflect the ratio of slow to fast fibers in DMD patient (>6 years). Fibroblast growth factor, transforming growth factor-β, and tumor necrosis factor-α could affect the secretion of myomiRs, suggesting that circulating myomiRs might reflect the effects of cytokines and growth factors on degenerating and regenerating muscles. Collectively, our data indicated that circulating myomiRs could serve as promising biomarkers for DMD diagnosis and disease progression.
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spelling pubmed-41215182014-08-14 Circulating Muscle-specific miRNAs in Duchenne Muscular Dystrophy Patients Li, Xihua Li, Yuying Zhao, Lei Zhang, Duo Yao, Xuan Zhang, Huihui Wang, Yu-cheng Wang, Xin-yi Xia, Hongfeng Yan, Jun Ying, Hao Mol Ther Nucleic Acids Original Article Noninvasive biomarkers with diagnostic value and prognostic applications have long been desired to replace muscle biopsy for Duchenne muscular dystrophy (DMD) patients. Growing evidence indicates that circulating microRNAs are biomarkers to assess pathophysiological status. Here, we show that the serum levels of six muscle-specific miRNAs (miR-1/206/133/499/208a/208b, also known as myomiRs) were all elevated in DMD patients (P < 0.01). The receiver operating characteristic curves of circulating miR-206, miR-499, miR-208b, and miR-133 levels reflected strong separation between Becker's muscular dystrophy (BMD) and DMD patients (P < 0.05). miR-206, miR-499, and miR-208b levels were positively correlated with both age and type IIc muscle fiber content in DMD patients (2–6 years), indicating that they might represent the stage of disease as well as the process of regeneration. miR-499 and miR-208b levels were correlated with slow and fast fiber content and might reflect the ratio of slow to fast fibers in DMD patient (>6 years). Fibroblast growth factor, transforming growth factor-β, and tumor necrosis factor-α could affect the secretion of myomiRs, suggesting that circulating myomiRs might reflect the effects of cytokines and growth factors on degenerating and regenerating muscles. Collectively, our data indicated that circulating myomiRs could serve as promising biomarkers for DMD diagnosis and disease progression. Nature Publishing Group 2014-07 2014-07-22 /pmc/articles/PMC4121518/ /pubmed/25050825 http://dx.doi.org/10.1038/mtna.2014.29 Text en Copyright © 2014 The American Society of Gene & Cell Therapy http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Li, Xihua
Li, Yuying
Zhao, Lei
Zhang, Duo
Yao, Xuan
Zhang, Huihui
Wang, Yu-cheng
Wang, Xin-yi
Xia, Hongfeng
Yan, Jun
Ying, Hao
Circulating Muscle-specific miRNAs in Duchenne Muscular Dystrophy Patients
title Circulating Muscle-specific miRNAs in Duchenne Muscular Dystrophy Patients
title_full Circulating Muscle-specific miRNAs in Duchenne Muscular Dystrophy Patients
title_fullStr Circulating Muscle-specific miRNAs in Duchenne Muscular Dystrophy Patients
title_full_unstemmed Circulating Muscle-specific miRNAs in Duchenne Muscular Dystrophy Patients
title_short Circulating Muscle-specific miRNAs in Duchenne Muscular Dystrophy Patients
title_sort circulating muscle-specific mirnas in duchenne muscular dystrophy patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121518/
https://www.ncbi.nlm.nih.gov/pubmed/25050825
http://dx.doi.org/10.1038/mtna.2014.29
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