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Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods

Spatiotemporal mapping of the position and orientation of nano-machinery inside complex and dynamic cellular environments is essential for the detailed understanding of many bio-physical processes. For the genuine observation of such biomolecular dynamics with high signal to noise ratio and reduced...

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Autores principales: Chaudhari, Kamalesh, Pradeep, Thalappil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121602/
https://www.ncbi.nlm.nih.gov/pubmed/25091698
http://dx.doi.org/10.1038/srep05948
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author Chaudhari, Kamalesh
Pradeep, Thalappil
author_facet Chaudhari, Kamalesh
Pradeep, Thalappil
author_sort Chaudhari, Kamalesh
collection PubMed
description Spatiotemporal mapping of the position and orientation of nano-machinery inside complex and dynamic cellular environments is essential for the detailed understanding of many bio-physical processes. For the genuine observation of such biomolecular dynamics with high signal to noise ratio and reduced disturbance from the labeling probes, reduction in the size of nano-bio labels and simplification of techniques for their observation are important. Here we achieve this using polarized dark field scattering micro-spectroscopy (PDFSMS), in its simplest form so that it is deployable in several experiments. We not only locate tiny gold nanorods (GNRs) of size 30 (length) × 10 nm (diameter) inside HEK293 cells but also demonstrate mapping of their in-situ polarization patterns using a novel method. Real time observations of rotating GNR with DFSMS and PDFSMS are used to resolve in-plane and out-of-plane rotational modes of GNR. We have shown that PDFSMS itself can provide complete information about the state of GNR. A step ahead, we demonstrate the application of PDFSMS to track three dimensional rotational dynamics of transferrin-conjugated GNRs inside live HEK293 cells. These first-time observations of the three dimensional intracellular rotational dynamics of tiny GNRs using PDFSMS present a new landmark in single particle scattering spectroscopy.
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spelling pubmed-41216022014-08-14 Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods Chaudhari, Kamalesh Pradeep, Thalappil Sci Rep Article Spatiotemporal mapping of the position and orientation of nano-machinery inside complex and dynamic cellular environments is essential for the detailed understanding of many bio-physical processes. For the genuine observation of such biomolecular dynamics with high signal to noise ratio and reduced disturbance from the labeling probes, reduction in the size of nano-bio labels and simplification of techniques for their observation are important. Here we achieve this using polarized dark field scattering micro-spectroscopy (PDFSMS), in its simplest form so that it is deployable in several experiments. We not only locate tiny gold nanorods (GNRs) of size 30 (length) × 10 nm (diameter) inside HEK293 cells but also demonstrate mapping of their in-situ polarization patterns using a novel method. Real time observations of rotating GNR with DFSMS and PDFSMS are used to resolve in-plane and out-of-plane rotational modes of GNR. We have shown that PDFSMS itself can provide complete information about the state of GNR. A step ahead, we demonstrate the application of PDFSMS to track three dimensional rotational dynamics of transferrin-conjugated GNRs inside live HEK293 cells. These first-time observations of the three dimensional intracellular rotational dynamics of tiny GNRs using PDFSMS present a new landmark in single particle scattering spectroscopy. Nature Publishing Group 2014-08-05 /pmc/articles/PMC4121602/ /pubmed/25091698 http://dx.doi.org/10.1038/srep05948 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Chaudhari, Kamalesh
Pradeep, Thalappil
Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods
title Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods
title_full Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods
title_fullStr Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods
title_full_unstemmed Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods
title_short Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods
title_sort spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121602/
https://www.ncbi.nlm.nih.gov/pubmed/25091698
http://dx.doi.org/10.1038/srep05948
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