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Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration
Polarized cell migration is essential for normal organism development and is also a critical component of cancer cell invasion and disease progression. Directional cell motility requires the coordination of dynamic cell–extracellular matrix interactions as well as repositioning of the Golgi apparatu...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121979/ https://www.ncbi.nlm.nih.gov/pubmed/25070956 http://dx.doi.org/10.1083/jcb.201403039 |
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author | Deakin, Nicholas O. Turner, Christopher E. |
author_facet | Deakin, Nicholas O. Turner, Christopher E. |
author_sort | Deakin, Nicholas O. |
collection | PubMed |
description | Polarized cell migration is essential for normal organism development and is also a critical component of cancer cell invasion and disease progression. Directional cell motility requires the coordination of dynamic cell–extracellular matrix interactions as well as repositioning of the Golgi apparatus, both of which can be controlled by the microtubule (MT) cytoskeleton. In this paper, we have identified a new and conserved role for the focal adhesion scaffold protein paxillin in regulating the posttranslational modification of the MT cytoskeleton through an inhibitory interaction with the α-tubulin deacetylase HDAC6. We also determined that through HDAC6-dependent regulation of the MT cytoskeleton, paxillin regulates both Golgi organelle integrity and polarized cell invasion and migration in both three-dimensional and two-dimensional matrix microenvironments. Importantly, these data reveal a fundamental role for paxillin in coordinating MT acetylation-dependent cell polarization and migration in both normal and transformed cells. |
format | Online Article Text |
id | pubmed-4121979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41219792015-02-04 Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration Deakin, Nicholas O. Turner, Christopher E. J Cell Biol Research Articles Polarized cell migration is essential for normal organism development and is also a critical component of cancer cell invasion and disease progression. Directional cell motility requires the coordination of dynamic cell–extracellular matrix interactions as well as repositioning of the Golgi apparatus, both of which can be controlled by the microtubule (MT) cytoskeleton. In this paper, we have identified a new and conserved role for the focal adhesion scaffold protein paxillin in regulating the posttranslational modification of the MT cytoskeleton through an inhibitory interaction with the α-tubulin deacetylase HDAC6. We also determined that through HDAC6-dependent regulation of the MT cytoskeleton, paxillin regulates both Golgi organelle integrity and polarized cell invasion and migration in both three-dimensional and two-dimensional matrix microenvironments. Importantly, these data reveal a fundamental role for paxillin in coordinating MT acetylation-dependent cell polarization and migration in both normal and transformed cells. The Rockefeller University Press 2014-08-04 /pmc/articles/PMC4121979/ /pubmed/25070956 http://dx.doi.org/10.1083/jcb.201403039 Text en © 2014 Deakin and Turner This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Deakin, Nicholas O. Turner, Christopher E. Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration |
title | Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration |
title_full | Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration |
title_fullStr | Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration |
title_full_unstemmed | Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration |
title_short | Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration |
title_sort | paxillin inhibits hdac6 to regulate microtubule acetylation, golgi structure, and polarized migration |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121979/ https://www.ncbi.nlm.nih.gov/pubmed/25070956 http://dx.doi.org/10.1083/jcb.201403039 |
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