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Laser speckle contrast imaging for intraoperative assessment of liver microcirculation: a clinical pilot study

BACKGROUND: Liver microcirculation can be affected by a wide variety of causes relevant to liver transplantation and resectional surgery. Intraoperative assessment of the microcirculation could possibly predict postoperative outcome. The present pilot study introduces laser speckle contrast imaging...

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Autores principales: Eriksson, Sam, Nilsson, Jan, Lindell, Gert, Sturesson, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4122554/
https://www.ncbi.nlm.nih.gov/pubmed/25114599
http://dx.doi.org/10.2147/MDER.S63393
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author Eriksson, Sam
Nilsson, Jan
Lindell, Gert
Sturesson, Christian
author_facet Eriksson, Sam
Nilsson, Jan
Lindell, Gert
Sturesson, Christian
author_sort Eriksson, Sam
collection PubMed
description BACKGROUND: Liver microcirculation can be affected by a wide variety of causes relevant to liver transplantation and resectional surgery. Intraoperative assessment of the microcirculation could possibly predict postoperative outcome. The present pilot study introduces laser speckle contrast imaging (LSCI) as a new clinical method for assessing liver microcirculation. METHODS: LSCI measurements of liver microcirculation were performed on ten patients undergoing liver resection. Measurements were made during apnea with and without liver blood inflow occlusion. Hepatic blood flow was assessed by subtracting zero inflow signal from the total signal. Zero inflow signal was obtained after hepatic artery and portal vein occlusion. Perfusion was expressed in laser speckle perfusion units, and intraindividual and interindividual variability in liver perfusion was investigated using the coefficient of variability. RESULTS: Hepatic microcirculation measurements were successfully made in all patients resulting in analyzable speckle contrast images. Mean hepatic blood flow was 410±36 laser speckle perfusion units. Zero inflow signal amounted to 40%±4% of the total signal. Intraindividual and interindividual coefficients of variability in liver perfusion were 25% and 28%, respectively. CONCLUSION: Under the conditions of this pilot study, LSCI allows rapid noncontact measurements of hepatic blood perfusion over wide areas. More studies are needed on methods of handling movement artifacts.
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spelling pubmed-41225542014-08-11 Laser speckle contrast imaging for intraoperative assessment of liver microcirculation: a clinical pilot study Eriksson, Sam Nilsson, Jan Lindell, Gert Sturesson, Christian Med Devices (Auckl) Original Research BACKGROUND: Liver microcirculation can be affected by a wide variety of causes relevant to liver transplantation and resectional surgery. Intraoperative assessment of the microcirculation could possibly predict postoperative outcome. The present pilot study introduces laser speckle contrast imaging (LSCI) as a new clinical method for assessing liver microcirculation. METHODS: LSCI measurements of liver microcirculation were performed on ten patients undergoing liver resection. Measurements were made during apnea with and without liver blood inflow occlusion. Hepatic blood flow was assessed by subtracting zero inflow signal from the total signal. Zero inflow signal was obtained after hepatic artery and portal vein occlusion. Perfusion was expressed in laser speckle perfusion units, and intraindividual and interindividual variability in liver perfusion was investigated using the coefficient of variability. RESULTS: Hepatic microcirculation measurements were successfully made in all patients resulting in analyzable speckle contrast images. Mean hepatic blood flow was 410±36 laser speckle perfusion units. Zero inflow signal amounted to 40%±4% of the total signal. Intraindividual and interindividual coefficients of variability in liver perfusion were 25% and 28%, respectively. CONCLUSION: Under the conditions of this pilot study, LSCI allows rapid noncontact measurements of hepatic blood perfusion over wide areas. More studies are needed on methods of handling movement artifacts. Dove Medical Press 2014-07-25 /pmc/articles/PMC4122554/ /pubmed/25114599 http://dx.doi.org/10.2147/MDER.S63393 Text en © 2014 Eriksson et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Eriksson, Sam
Nilsson, Jan
Lindell, Gert
Sturesson, Christian
Laser speckle contrast imaging for intraoperative assessment of liver microcirculation: a clinical pilot study
title Laser speckle contrast imaging for intraoperative assessment of liver microcirculation: a clinical pilot study
title_full Laser speckle contrast imaging for intraoperative assessment of liver microcirculation: a clinical pilot study
title_fullStr Laser speckle contrast imaging for intraoperative assessment of liver microcirculation: a clinical pilot study
title_full_unstemmed Laser speckle contrast imaging for intraoperative assessment of liver microcirculation: a clinical pilot study
title_short Laser speckle contrast imaging for intraoperative assessment of liver microcirculation: a clinical pilot study
title_sort laser speckle contrast imaging for intraoperative assessment of liver microcirculation: a clinical pilot study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4122554/
https://www.ncbi.nlm.nih.gov/pubmed/25114599
http://dx.doi.org/10.2147/MDER.S63393
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