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In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry

Here we describe the application of a new click chemistry method for fluorescent tracking of protein synthesis in individual microorganisms within environmental samples. This technique, termed bioorthogonal non-canonical amino acid tagging (BONCAT), is based on the in vivo incorporation of the non-c...

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Detalles Bibliográficos
Autores principales:	Hatzenpichler, Roland, Scheller, Silvan, Tavormina, Patricia L, Babin, Brett M, Tirrell, David A, Orphan, Victoria J
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BlackWell Publishing Ltd 2014
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4122687/ https://www.ncbi.nlm.nih.gov/pubmed/24571640 http://dx.doi.org/10.1111/1462-2920.12436

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