Cargando…
Uterine Rbpj is required for embryonic-uterine orientation and decidual remodeling via Notch pathway-independent and -dependent mechanisms
Coordinated uterine-embryonic axis formation and decidual remodeling are hallmarks of mammalian post-implantation embryo development. Embryonic-uterine orientation is determined at initial implantation and synchronized with decidual development. However, the molecular mechanisms controlling these ev...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4123295/ https://www.ncbi.nlm.nih.gov/pubmed/24971735 http://dx.doi.org/10.1038/cr.2014.82 |
_version_ | 1782329490070831104 |
---|---|
author | Zhang, Shuang Kong, Shuangbo Wang, Bingyan Cheng, Xiaohong Chen, Yongjie Wu, Weiwei Wang, Qiang Shi, Junchao Zhang, Ying Wang, Shumin Lu, Jinhua Lydon, John P DeMayo, Francesco Pear, Warren S Han, Hua Lin, Haiyan Li, Lei Wang, Hongmei Wang, Yan-ling Li, Bing Chen, Qi Duan, Enkui Wang, Haibin |
author_facet | Zhang, Shuang Kong, Shuangbo Wang, Bingyan Cheng, Xiaohong Chen, Yongjie Wu, Weiwei Wang, Qiang Shi, Junchao Zhang, Ying Wang, Shumin Lu, Jinhua Lydon, John P DeMayo, Francesco Pear, Warren S Han, Hua Lin, Haiyan Li, Lei Wang, Hongmei Wang, Yan-ling Li, Bing Chen, Qi Duan, Enkui Wang, Haibin |
author_sort | Zhang, Shuang |
collection | PubMed |
description | Coordinated uterine-embryonic axis formation and decidual remodeling are hallmarks of mammalian post-implantation embryo development. Embryonic-uterine orientation is determined at initial implantation and synchronized with decidual development. However, the molecular mechanisms controlling these events remain elusive despite its discovery a long time ago. In the present study, we found that uterine-specific deletion of Rbpj, the nuclear transducer of Notch signaling, resulted in abnormal embryonic-uterine orientation and decidual patterning at post-implantation stages, leading to substantial embryo loss. We further revealed that prior to embryo attachment, Rbpj confers on-time uterine lumen shape transformation via physically interacting with uterine estrogen receptor (ERα) in a Notch pathway-independent manner, which is essential for the initial establishment of embryo orientation in alignment with uterine axis. While at post-implantation stages, Rbpj directly regulates the expression of uterine matrix metalloproteinase in a Notch pathway-dependent manner, which is required for normal post-implantation decidual remodeling. These results demonstrate that uterine Rbpj is essential for normal embryo development via instructing the initial embryonic-uterine orientation and ensuring normal decidual patterning in a stage-specific manner. Our data also substantiate the concept that normal mammalian embryonic-uterine orientation requires proper guidance from developmentally controlled uterine signaling. |
format | Online Article Text |
id | pubmed-4123295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-41232952014-08-15 Uterine Rbpj is required for embryonic-uterine orientation and decidual remodeling via Notch pathway-independent and -dependent mechanisms Zhang, Shuang Kong, Shuangbo Wang, Bingyan Cheng, Xiaohong Chen, Yongjie Wu, Weiwei Wang, Qiang Shi, Junchao Zhang, Ying Wang, Shumin Lu, Jinhua Lydon, John P DeMayo, Francesco Pear, Warren S Han, Hua Lin, Haiyan Li, Lei Wang, Hongmei Wang, Yan-ling Li, Bing Chen, Qi Duan, Enkui Wang, Haibin Cell Res Original Article Coordinated uterine-embryonic axis formation and decidual remodeling are hallmarks of mammalian post-implantation embryo development. Embryonic-uterine orientation is determined at initial implantation and synchronized with decidual development. However, the molecular mechanisms controlling these events remain elusive despite its discovery a long time ago. In the present study, we found that uterine-specific deletion of Rbpj, the nuclear transducer of Notch signaling, resulted in abnormal embryonic-uterine orientation and decidual patterning at post-implantation stages, leading to substantial embryo loss. We further revealed that prior to embryo attachment, Rbpj confers on-time uterine lumen shape transformation via physically interacting with uterine estrogen receptor (ERα) in a Notch pathway-independent manner, which is essential for the initial establishment of embryo orientation in alignment with uterine axis. While at post-implantation stages, Rbpj directly regulates the expression of uterine matrix metalloproteinase in a Notch pathway-dependent manner, which is required for normal post-implantation decidual remodeling. These results demonstrate that uterine Rbpj is essential for normal embryo development via instructing the initial embryonic-uterine orientation and ensuring normal decidual patterning in a stage-specific manner. Our data also substantiate the concept that normal mammalian embryonic-uterine orientation requires proper guidance from developmentally controlled uterine signaling. Nature Publishing Group 2014-08 2014-06-27 /pmc/articles/PMC4123295/ /pubmed/24971735 http://dx.doi.org/10.1038/cr.2014.82 Text en Copyright © 2014 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0 |
spellingShingle | Original Article Zhang, Shuang Kong, Shuangbo Wang, Bingyan Cheng, Xiaohong Chen, Yongjie Wu, Weiwei Wang, Qiang Shi, Junchao Zhang, Ying Wang, Shumin Lu, Jinhua Lydon, John P DeMayo, Francesco Pear, Warren S Han, Hua Lin, Haiyan Li, Lei Wang, Hongmei Wang, Yan-ling Li, Bing Chen, Qi Duan, Enkui Wang, Haibin Uterine Rbpj is required for embryonic-uterine orientation and decidual remodeling via Notch pathway-independent and -dependent mechanisms |
title | Uterine Rbpj is required for embryonic-uterine orientation and decidual remodeling via Notch pathway-independent and -dependent mechanisms |
title_full | Uterine Rbpj is required for embryonic-uterine orientation and decidual remodeling via Notch pathway-independent and -dependent mechanisms |
title_fullStr | Uterine Rbpj is required for embryonic-uterine orientation and decidual remodeling via Notch pathway-independent and -dependent mechanisms |
title_full_unstemmed | Uterine Rbpj is required for embryonic-uterine orientation and decidual remodeling via Notch pathway-independent and -dependent mechanisms |
title_short | Uterine Rbpj is required for embryonic-uterine orientation and decidual remodeling via Notch pathway-independent and -dependent mechanisms |
title_sort | uterine rbpj is required for embryonic-uterine orientation and decidual remodeling via notch pathway-independent and -dependent mechanisms |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4123295/ https://www.ncbi.nlm.nih.gov/pubmed/24971735 http://dx.doi.org/10.1038/cr.2014.82 |
work_keys_str_mv | AT zhangshuang uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT kongshuangbo uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT wangbingyan uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT chengxiaohong uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT chenyongjie uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT wuweiwei uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT wangqiang uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT shijunchao uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT zhangying uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT wangshumin uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT lujinhua uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT lydonjohnp uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT demayofrancesco uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT pearwarrens uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT hanhua uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT linhaiyan uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT lilei uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT wanghongmei uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT wangyanling uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT libing uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT chenqi uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT duanenkui uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms AT wanghaibin uterinerbpjisrequiredforembryonicuterineorientationanddecidualremodelingvianotchpathwayindependentanddependentmechanisms |