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Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus
BACKGROUND: Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China. Genome sequencing of the pathogen will provide important information for globally understanding its virulence mechanism. RESULTS: We report the genome sequences of a hig...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124159/ https://www.ncbi.nlm.nih.gov/pubmed/25056288 http://dx.doi.org/10.1186/1471-2164-15-627 |
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author | Gao, Shigang Li, Yaqian Gao, Jinxin Suo, Yujuan Fu, Kehe Li, Yingying Chen, Jie |
author_facet | Gao, Shigang Li, Yaqian Gao, Jinxin Suo, Yujuan Fu, Kehe Li, Yingying Chen, Jie |
author_sort | Gao, Shigang |
collection | PubMed |
description | BACKGROUND: Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China. Genome sequencing of the pathogen will provide important information for globally understanding its virulence mechanism. RESULTS: We report the genome sequences of a highly virulent C. lunata strain. Phylogenomic analysis indicates that C. lunata was evolved from Bipolaris maydis (Cochliobolus heterostrophus). The highly virulent strain has a high potential to evolve into other pathogenic stains based on analyses on transposases and repeat-induced point mutations. C. lunata has a smaller proportion of secreted proteins as well as B. maydis than entomopathogenic fungi. C. lunata and B. maydis have a similar proportion of protein-encoding genes highly homologous to experimentally proven pathogenic genes from pathogen-host interaction database. However, relative to B. maydis, C. lunata possesses not only many expanded protein families including MFS transporters, G-protein coupled receptors, protein kinases and proteases for transport, signal transduction or degradation, but also many contracted families including cytochrome P450, lipases, glycoside hydrolases and polyketide synthases for detoxification, hydrolysis or secondary metabolites biosynthesis, which are expected to be crucial for the fungal survival in varied stress environments. Comparative transcriptome analysis between a lowly virulent C. lunata strain and its virulence-increased variant induced by resistant host selection reveals that the virulence increase of the pathogen is related to pathways of toxin and melanin biosynthesis in stress environments, and that the two pathways probably have some overlaps. CONCLUSIONS: The data will facilitate a full revelation of pathogenic mechanism and a better understanding of virulence differentiation of C. lunata. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-627) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4124159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41241592014-08-12 Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus Gao, Shigang Li, Yaqian Gao, Jinxin Suo, Yujuan Fu, Kehe Li, Yingying Chen, Jie BMC Genomics Research Article BACKGROUND: Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China. Genome sequencing of the pathogen will provide important information for globally understanding its virulence mechanism. RESULTS: We report the genome sequences of a highly virulent C. lunata strain. Phylogenomic analysis indicates that C. lunata was evolved from Bipolaris maydis (Cochliobolus heterostrophus). The highly virulent strain has a high potential to evolve into other pathogenic stains based on analyses on transposases and repeat-induced point mutations. C. lunata has a smaller proportion of secreted proteins as well as B. maydis than entomopathogenic fungi. C. lunata and B. maydis have a similar proportion of protein-encoding genes highly homologous to experimentally proven pathogenic genes from pathogen-host interaction database. However, relative to B. maydis, C. lunata possesses not only many expanded protein families including MFS transporters, G-protein coupled receptors, protein kinases and proteases for transport, signal transduction or degradation, but also many contracted families including cytochrome P450, lipases, glycoside hydrolases and polyketide synthases for detoxification, hydrolysis or secondary metabolites biosynthesis, which are expected to be crucial for the fungal survival in varied stress environments. Comparative transcriptome analysis between a lowly virulent C. lunata strain and its virulence-increased variant induced by resistant host selection reveals that the virulence increase of the pathogen is related to pathways of toxin and melanin biosynthesis in stress environments, and that the two pathways probably have some overlaps. CONCLUSIONS: The data will facilitate a full revelation of pathogenic mechanism and a better understanding of virulence differentiation of C. lunata. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-627) contains supplementary material, which is available to authorized users. BioMed Central 2014-07-24 /pmc/articles/PMC4124159/ /pubmed/25056288 http://dx.doi.org/10.1186/1471-2164-15-627 Text en © Gao et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Gao, Shigang Li, Yaqian Gao, Jinxin Suo, Yujuan Fu, Kehe Li, Yingying Chen, Jie Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus |
title | Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus |
title_full | Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus |
title_fullStr | Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus |
title_full_unstemmed | Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus |
title_short | Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus |
title_sort | genome sequence and virulence variation-related transcriptome profiles of curvularia lunata, an important maize pathogenic fungus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124159/ https://www.ncbi.nlm.nih.gov/pubmed/25056288 http://dx.doi.org/10.1186/1471-2164-15-627 |
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