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Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus

BACKGROUND: Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China. Genome sequencing of the pathogen will provide important information for globally understanding its virulence mechanism. RESULTS: We report the genome sequences of a hig...

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Autores principales: Gao, Shigang, Li, Yaqian, Gao, Jinxin, Suo, Yujuan, Fu, Kehe, Li, Yingying, Chen, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124159/
https://www.ncbi.nlm.nih.gov/pubmed/25056288
http://dx.doi.org/10.1186/1471-2164-15-627
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author Gao, Shigang
Li, Yaqian
Gao, Jinxin
Suo, Yujuan
Fu, Kehe
Li, Yingying
Chen, Jie
author_facet Gao, Shigang
Li, Yaqian
Gao, Jinxin
Suo, Yujuan
Fu, Kehe
Li, Yingying
Chen, Jie
author_sort Gao, Shigang
collection PubMed
description BACKGROUND: Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China. Genome sequencing of the pathogen will provide important information for globally understanding its virulence mechanism. RESULTS: We report the genome sequences of a highly virulent C. lunata strain. Phylogenomic analysis indicates that C. lunata was evolved from Bipolaris maydis (Cochliobolus heterostrophus). The highly virulent strain has a high potential to evolve into other pathogenic stains based on analyses on transposases and repeat-induced point mutations. C. lunata has a smaller proportion of secreted proteins as well as B. maydis than entomopathogenic fungi. C. lunata and B. maydis have a similar proportion of protein-encoding genes highly homologous to experimentally proven pathogenic genes from pathogen-host interaction database. However, relative to B. maydis, C. lunata possesses not only many expanded protein families including MFS transporters, G-protein coupled receptors, protein kinases and proteases for transport, signal transduction or degradation, but also many contracted families including cytochrome P450, lipases, glycoside hydrolases and polyketide synthases for detoxification, hydrolysis or secondary metabolites biosynthesis, which are expected to be crucial for the fungal survival in varied stress environments. Comparative transcriptome analysis between a lowly virulent C. lunata strain and its virulence-increased variant induced by resistant host selection reveals that the virulence increase of the pathogen is related to pathways of toxin and melanin biosynthesis in stress environments, and that the two pathways probably have some overlaps. CONCLUSIONS: The data will facilitate a full revelation of pathogenic mechanism and a better understanding of virulence differentiation of C. lunata. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-627) contains supplementary material, which is available to authorized users.
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spelling pubmed-41241592014-08-12 Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus Gao, Shigang Li, Yaqian Gao, Jinxin Suo, Yujuan Fu, Kehe Li, Yingying Chen, Jie BMC Genomics Research Article BACKGROUND: Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China. Genome sequencing of the pathogen will provide important information for globally understanding its virulence mechanism. RESULTS: We report the genome sequences of a highly virulent C. lunata strain. Phylogenomic analysis indicates that C. lunata was evolved from Bipolaris maydis (Cochliobolus heterostrophus). The highly virulent strain has a high potential to evolve into other pathogenic stains based on analyses on transposases and repeat-induced point mutations. C. lunata has a smaller proportion of secreted proteins as well as B. maydis than entomopathogenic fungi. C. lunata and B. maydis have a similar proportion of protein-encoding genes highly homologous to experimentally proven pathogenic genes from pathogen-host interaction database. However, relative to B. maydis, C. lunata possesses not only many expanded protein families including MFS transporters, G-protein coupled receptors, protein kinases and proteases for transport, signal transduction or degradation, but also many contracted families including cytochrome P450, lipases, glycoside hydrolases and polyketide synthases for detoxification, hydrolysis or secondary metabolites biosynthesis, which are expected to be crucial for the fungal survival in varied stress environments. Comparative transcriptome analysis between a lowly virulent C. lunata strain and its virulence-increased variant induced by resistant host selection reveals that the virulence increase of the pathogen is related to pathways of toxin and melanin biosynthesis in stress environments, and that the two pathways probably have some overlaps. CONCLUSIONS: The data will facilitate a full revelation of pathogenic mechanism and a better understanding of virulence differentiation of C. lunata. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-627) contains supplementary material, which is available to authorized users. BioMed Central 2014-07-24 /pmc/articles/PMC4124159/ /pubmed/25056288 http://dx.doi.org/10.1186/1471-2164-15-627 Text en © Gao et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Gao, Shigang
Li, Yaqian
Gao, Jinxin
Suo, Yujuan
Fu, Kehe
Li, Yingying
Chen, Jie
Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus
title Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus
title_full Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus
title_fullStr Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus
title_full_unstemmed Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus
title_short Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus
title_sort genome sequence and virulence variation-related transcriptome profiles of curvularia lunata, an important maize pathogenic fungus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124159/
https://www.ncbi.nlm.nih.gov/pubmed/25056288
http://dx.doi.org/10.1186/1471-2164-15-627
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