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Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo
BACKGROUND: Regulation of gene expression plays a central role in embryonic development. Early stages are controlled by gametic transcripts, which are subsequently substituted with transcripts from the genome of the zygote. Transcriptomic analyses provide an efficient approach to explore the tempora...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124161/ https://www.ncbi.nlm.nih.gov/pubmed/25023375 http://dx.doi.org/10.1186/1471-2164-15-594 |
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author | Škugor, Adrijana Krasnov, Aleksei Andersen, Øivind |
author_facet | Škugor, Adrijana Krasnov, Aleksei Andersen, Øivind |
author_sort | Škugor, Adrijana |
collection | PubMed |
description | BACKGROUND: Regulation of gene expression plays a central role in embryonic development. Early stages are controlled by gametic transcripts, which are subsequently substituted with transcripts from the genome of the zygote. Transcriptomic analyses provide an efficient approach to explore the temporal gene expression profiles in embryos and to search for the developmental regulators. We report a study of early Atlantic cod development that used a genome-wide oligonucleotide microarray to examine the composition and putative roles of polyadenylated transcripts. RESULTS: The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula transition and segmentation. Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism, signal perception and transduction, RNA processing, cell cycle, defense against pathogens and DNA damage. Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). Prezygotic transcripts included multiple regulators that are most likely involved in developmental processes that take place long after fertilization, such as components of ErbB, hedgehog, notch, retinoid, TGFb, VEGF and Wnt signaling pathways, as well as transcripts involved in the development of nervous system. The major event of MBT was the activation of a large group of histones and other genes that modify chromatin structure preceding massive gene expression changes. A hallmark of events observed during segmentation was the induction of multiple transcription factors, including a large group of homeobox proteins in pace with decay of a large fraction of maternal transcripts. Microarray analyses detected a suite of master developmental regulators that control differentiation and maintenance of diverse cell lineages. CONCLUSIONS: Transcriptome profiling of the early stages in Atlantic cod revealed the presence of transcripts involved in patterning and development of tissues and organs long before activation of the zygotic genome. The switch from maternal to zygotic developmental programs is associated with large-scale modification of chromosomes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-594) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4124161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41241612014-08-12 Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo Škugor, Adrijana Krasnov, Aleksei Andersen, Øivind BMC Genomics Research Article BACKGROUND: Regulation of gene expression plays a central role in embryonic development. Early stages are controlled by gametic transcripts, which are subsequently substituted with transcripts from the genome of the zygote. Transcriptomic analyses provide an efficient approach to explore the temporal gene expression profiles in embryos and to search for the developmental regulators. We report a study of early Atlantic cod development that used a genome-wide oligonucleotide microarray to examine the composition and putative roles of polyadenylated transcripts. RESULTS: The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula transition and segmentation. Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism, signal perception and transduction, RNA processing, cell cycle, defense against pathogens and DNA damage. Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). Prezygotic transcripts included multiple regulators that are most likely involved in developmental processes that take place long after fertilization, such as components of ErbB, hedgehog, notch, retinoid, TGFb, VEGF and Wnt signaling pathways, as well as transcripts involved in the development of nervous system. The major event of MBT was the activation of a large group of histones and other genes that modify chromatin structure preceding massive gene expression changes. A hallmark of events observed during segmentation was the induction of multiple transcription factors, including a large group of homeobox proteins in pace with decay of a large fraction of maternal transcripts. Microarray analyses detected a suite of master developmental regulators that control differentiation and maintenance of diverse cell lineages. CONCLUSIONS: Transcriptome profiling of the early stages in Atlantic cod revealed the presence of transcripts involved in patterning and development of tissues and organs long before activation of the zygotic genome. The switch from maternal to zygotic developmental programs is associated with large-scale modification of chromosomes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-594) contains supplementary material, which is available to authorized users. BioMed Central 2014-07-14 /pmc/articles/PMC4124161/ /pubmed/25023375 http://dx.doi.org/10.1186/1471-2164-15-594 Text en © Škugor et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Škugor, Adrijana Krasnov, Aleksei Andersen, Øivind Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo |
title | Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo |
title_full | Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo |
title_fullStr | Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo |
title_full_unstemmed | Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo |
title_short | Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo |
title_sort | genome-wide microarray analysis of atlantic cod (gadus morhua) oocyte and embryo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124161/ https://www.ncbi.nlm.nih.gov/pubmed/25023375 http://dx.doi.org/10.1186/1471-2164-15-594 |
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