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T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy

Aims. Normal human pregnancy is a complex process of many immunoregulatory mechanisms which protect fetus from the activation of the maternal immune system. The aim of the study was to investigate the apoptosis of lymphocytes in peripheral blood of normal pregnant patients and healthy nonpregnant wo...

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Autores principales: Darmochwal-Kolarz, Dorota, Sobczak, Ewelina, Pozarowski, Piotr, Kolarz, Bogdan, Rolinski, Jacek, Oleszczuk, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124785/
https://www.ncbi.nlm.nih.gov/pubmed/25133196
http://dx.doi.org/10.1155/2014/670524
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author Darmochwal-Kolarz, Dorota
Sobczak, Ewelina
Pozarowski, Piotr
Kolarz, Bogdan
Rolinski, Jacek
Oleszczuk, Jan
author_facet Darmochwal-Kolarz, Dorota
Sobczak, Ewelina
Pozarowski, Piotr
Kolarz, Bogdan
Rolinski, Jacek
Oleszczuk, Jan
author_sort Darmochwal-Kolarz, Dorota
collection PubMed
description Aims. Normal human pregnancy is a complex process of many immunoregulatory mechanisms which protect fetus from the activation of the maternal immune system. The aim of the study was to investigate the apoptosis of lymphocytes in peripheral blood of normal pregnant patients and healthy nonpregnant women. Methods. Sixty pregnant women and 17 nonpregnant women were included in the study. Lymphocytes were isolated and labeled with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies. Apoptosis was detected by CMXRos staining and analyzed using the flow cytometric method. Results. We found significantly higher apoptosis of total lymphocytes in peripheral blood of pregnant patients when compared to healthy nonpregnant women. The percentage of apoptotic T CD3(+)CD8(+) cells in the first trimester was significantly higher when compared to the third trimester of normal pregnancy. The ratio of T CD3(+)CD4(+) : T CD3(+)CD8(+) apoptotic lymphocytes was significantly lower in the first trimester when compared to other trimesters of pregnancy and to both of the phases of the menstrual cycle. Conclusions. The higher apoptosis of T CD3(+)CD8(+) lymphocytes and the lower ratio of T CD3(+)CD4(+) : T CD3(+)CD8(+) apoptotic cells in the first trimester of normal pregnancy may suggest a higher susceptibility of T CD3(+)CD8(+) cells for apoptosis as a protective mechanism at the early stage of pregnancy.
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spelling pubmed-41247852014-08-17 T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy Darmochwal-Kolarz, Dorota Sobczak, Ewelina Pozarowski, Piotr Kolarz, Bogdan Rolinski, Jacek Oleszczuk, Jan J Immunol Res Research Article Aims. Normal human pregnancy is a complex process of many immunoregulatory mechanisms which protect fetus from the activation of the maternal immune system. The aim of the study was to investigate the apoptosis of lymphocytes in peripheral blood of normal pregnant patients and healthy nonpregnant women. Methods. Sixty pregnant women and 17 nonpregnant women were included in the study. Lymphocytes were isolated and labeled with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies. Apoptosis was detected by CMXRos staining and analyzed using the flow cytometric method. Results. We found significantly higher apoptosis of total lymphocytes in peripheral blood of pregnant patients when compared to healthy nonpregnant women. The percentage of apoptotic T CD3(+)CD8(+) cells in the first trimester was significantly higher when compared to the third trimester of normal pregnancy. The ratio of T CD3(+)CD4(+) : T CD3(+)CD8(+) apoptotic lymphocytes was significantly lower in the first trimester when compared to other trimesters of pregnancy and to both of the phases of the menstrual cycle. Conclusions. The higher apoptosis of T CD3(+)CD8(+) lymphocytes and the lower ratio of T CD3(+)CD4(+) : T CD3(+)CD8(+) apoptotic cells in the first trimester of normal pregnancy may suggest a higher susceptibility of T CD3(+)CD8(+) cells for apoptosis as a protective mechanism at the early stage of pregnancy. Hindawi Publishing Corporation 2014 2014-07-20 /pmc/articles/PMC4124785/ /pubmed/25133196 http://dx.doi.org/10.1155/2014/670524 Text en Copyright © 2014 Dorota Darmochwal-Kolarz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Darmochwal-Kolarz, Dorota
Sobczak, Ewelina
Pozarowski, Piotr
Kolarz, Bogdan
Rolinski, Jacek
Oleszczuk, Jan
T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy
title T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy
title_full T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy
title_fullStr T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy
title_full_unstemmed T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy
title_short T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy
title_sort t cd3(+)cd8(+) lymphocytes are more susceptible for apoptosis in the first trimester of normal human pregnancy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124785/
https://www.ncbi.nlm.nih.gov/pubmed/25133196
http://dx.doi.org/10.1155/2014/670524
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