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T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy
Aims. Normal human pregnancy is a complex process of many immunoregulatory mechanisms which protect fetus from the activation of the maternal immune system. The aim of the study was to investigate the apoptosis of lymphocytes in peripheral blood of normal pregnant patients and healthy nonpregnant wo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124785/ https://www.ncbi.nlm.nih.gov/pubmed/25133196 http://dx.doi.org/10.1155/2014/670524 |
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author | Darmochwal-Kolarz, Dorota Sobczak, Ewelina Pozarowski, Piotr Kolarz, Bogdan Rolinski, Jacek Oleszczuk, Jan |
author_facet | Darmochwal-Kolarz, Dorota Sobczak, Ewelina Pozarowski, Piotr Kolarz, Bogdan Rolinski, Jacek Oleszczuk, Jan |
author_sort | Darmochwal-Kolarz, Dorota |
collection | PubMed |
description | Aims. Normal human pregnancy is a complex process of many immunoregulatory mechanisms which protect fetus from the activation of the maternal immune system. The aim of the study was to investigate the apoptosis of lymphocytes in peripheral blood of normal pregnant patients and healthy nonpregnant women. Methods. Sixty pregnant women and 17 nonpregnant women were included in the study. Lymphocytes were isolated and labeled with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies. Apoptosis was detected by CMXRos staining and analyzed using the flow cytometric method. Results. We found significantly higher apoptosis of total lymphocytes in peripheral blood of pregnant patients when compared to healthy nonpregnant women. The percentage of apoptotic T CD3(+)CD8(+) cells in the first trimester was significantly higher when compared to the third trimester of normal pregnancy. The ratio of T CD3(+)CD4(+) : T CD3(+)CD8(+) apoptotic lymphocytes was significantly lower in the first trimester when compared to other trimesters of pregnancy and to both of the phases of the menstrual cycle. Conclusions. The higher apoptosis of T CD3(+)CD8(+) lymphocytes and the lower ratio of T CD3(+)CD4(+) : T CD3(+)CD8(+) apoptotic cells in the first trimester of normal pregnancy may suggest a higher susceptibility of T CD3(+)CD8(+) cells for apoptosis as a protective mechanism at the early stage of pregnancy. |
format | Online Article Text |
id | pubmed-4124785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41247852014-08-17 T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy Darmochwal-Kolarz, Dorota Sobczak, Ewelina Pozarowski, Piotr Kolarz, Bogdan Rolinski, Jacek Oleszczuk, Jan J Immunol Res Research Article Aims. Normal human pregnancy is a complex process of many immunoregulatory mechanisms which protect fetus from the activation of the maternal immune system. The aim of the study was to investigate the apoptosis of lymphocytes in peripheral blood of normal pregnant patients and healthy nonpregnant women. Methods. Sixty pregnant women and 17 nonpregnant women were included in the study. Lymphocytes were isolated and labeled with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies. Apoptosis was detected by CMXRos staining and analyzed using the flow cytometric method. Results. We found significantly higher apoptosis of total lymphocytes in peripheral blood of pregnant patients when compared to healthy nonpregnant women. The percentage of apoptotic T CD3(+)CD8(+) cells in the first trimester was significantly higher when compared to the third trimester of normal pregnancy. The ratio of T CD3(+)CD4(+) : T CD3(+)CD8(+) apoptotic lymphocytes was significantly lower in the first trimester when compared to other trimesters of pregnancy and to both of the phases of the menstrual cycle. Conclusions. The higher apoptosis of T CD3(+)CD8(+) lymphocytes and the lower ratio of T CD3(+)CD4(+) : T CD3(+)CD8(+) apoptotic cells in the first trimester of normal pregnancy may suggest a higher susceptibility of T CD3(+)CD8(+) cells for apoptosis as a protective mechanism at the early stage of pregnancy. Hindawi Publishing Corporation 2014 2014-07-20 /pmc/articles/PMC4124785/ /pubmed/25133196 http://dx.doi.org/10.1155/2014/670524 Text en Copyright © 2014 Dorota Darmochwal-Kolarz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Darmochwal-Kolarz, Dorota Sobczak, Ewelina Pozarowski, Piotr Kolarz, Bogdan Rolinski, Jacek Oleszczuk, Jan T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy |
title | T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy |
title_full | T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy |
title_fullStr | T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy |
title_full_unstemmed | T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy |
title_short | T CD3(+)CD8(+) Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy |
title_sort | t cd3(+)cd8(+) lymphocytes are more susceptible for apoptosis in the first trimester of normal human pregnancy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124785/ https://www.ncbi.nlm.nih.gov/pubmed/25133196 http://dx.doi.org/10.1155/2014/670524 |
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