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Salivary Gland Proteome Analysis Reveals Modulation of Anopheline Unique Proteins in Insensitive Acetylcholinesterase Resistant Anopheles gambiae Mosquitoes

Insensitive acetylcholinesterase resistance due to a mutation in the acetylcholinesterase (ace) encoding ace-1 gene confers cross-resistance to organophosphate and carbamate insecticides in Anopheles gambiae populations from Central and West Africa. This mutation is associated with a strong genetic...

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Autores principales: Cornelie, Sylvie, Rossignol, Marie, Seveno, Martial, Demettre, Edith, Mouchet, François, Djègbè, Innocent, Marin, Philippe, Chandre, Fabrice, Corbel, Vincent, Remoué, Franck, Mathieu-Daudé, Françoise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125145/
https://www.ncbi.nlm.nih.gov/pubmed/25102176
http://dx.doi.org/10.1371/journal.pone.0103816
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author Cornelie, Sylvie
Rossignol, Marie
Seveno, Martial
Demettre, Edith
Mouchet, François
Djègbè, Innocent
Marin, Philippe
Chandre, Fabrice
Corbel, Vincent
Remoué, Franck
Mathieu-Daudé, Françoise
author_facet Cornelie, Sylvie
Rossignol, Marie
Seveno, Martial
Demettre, Edith
Mouchet, François
Djègbè, Innocent
Marin, Philippe
Chandre, Fabrice
Corbel, Vincent
Remoué, Franck
Mathieu-Daudé, Françoise
author_sort Cornelie, Sylvie
collection PubMed
description Insensitive acetylcholinesterase resistance due to a mutation in the acetylcholinesterase (ace) encoding ace-1 gene confers cross-resistance to organophosphate and carbamate insecticides in Anopheles gambiae populations from Central and West Africa. This mutation is associated with a strong genetic cost revealed through alterations of some life history traits but little is known about the physiological and behavioural changes in insects bearing the ace-1(R) allele. Comparative analysis of the salivary gland contents between An. gambiae susceptible and ace-1(R) resistant strains was carried out to charaterize factors that could be involved in modifications of blood meal process, trophic behaviour or pathogen interaction in the insecticide-resistant mosquitoes. Differential analysis of the salivary gland protein profiles revealed differences in abundance for several proteins, two of them showing major differences between the two strains. These two proteins identified as saglin and TRIO are salivary gland-1 related proteins, a family unique to anopheline mosquitoes, one of them playing a crucial role in salivary gland invasion by Plasmodium falciparum sporozoites. Differential expression of two other proteins previously identified in the Anopheles sialome was also observed. The differentially regulated proteins are involved in pathogen invasion, blood feeding process, and protection against oxidation, relevant steps in the outcome of malaria infection. Further functional studies and insect behaviour experiments would confirm the impact of the modification of the sialome composition on blood feeding and pathogen transmission abilities of the resistant mosquitoes. The data supports the hypothesis of alterations linked to insecticide resistance in the biology of the primary vector of human malaria in Africa.
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spelling pubmed-41251452014-08-12 Salivary Gland Proteome Analysis Reveals Modulation of Anopheline Unique Proteins in Insensitive Acetylcholinesterase Resistant Anopheles gambiae Mosquitoes Cornelie, Sylvie Rossignol, Marie Seveno, Martial Demettre, Edith Mouchet, François Djègbè, Innocent Marin, Philippe Chandre, Fabrice Corbel, Vincent Remoué, Franck Mathieu-Daudé, Françoise PLoS One Research Article Insensitive acetylcholinesterase resistance due to a mutation in the acetylcholinesterase (ace) encoding ace-1 gene confers cross-resistance to organophosphate and carbamate insecticides in Anopheles gambiae populations from Central and West Africa. This mutation is associated with a strong genetic cost revealed through alterations of some life history traits but little is known about the physiological and behavioural changes in insects bearing the ace-1(R) allele. Comparative analysis of the salivary gland contents between An. gambiae susceptible and ace-1(R) resistant strains was carried out to charaterize factors that could be involved in modifications of blood meal process, trophic behaviour or pathogen interaction in the insecticide-resistant mosquitoes. Differential analysis of the salivary gland protein profiles revealed differences in abundance for several proteins, two of them showing major differences between the two strains. These two proteins identified as saglin and TRIO are salivary gland-1 related proteins, a family unique to anopheline mosquitoes, one of them playing a crucial role in salivary gland invasion by Plasmodium falciparum sporozoites. Differential expression of two other proteins previously identified in the Anopheles sialome was also observed. The differentially regulated proteins are involved in pathogen invasion, blood feeding process, and protection against oxidation, relevant steps in the outcome of malaria infection. Further functional studies and insect behaviour experiments would confirm the impact of the modification of the sialome composition on blood feeding and pathogen transmission abilities of the resistant mosquitoes. The data supports the hypothesis of alterations linked to insecticide resistance in the biology of the primary vector of human malaria in Africa. Public Library of Science 2014-08-07 /pmc/articles/PMC4125145/ /pubmed/25102176 http://dx.doi.org/10.1371/journal.pone.0103816 Text en © 2014 Cornelie et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cornelie, Sylvie
Rossignol, Marie
Seveno, Martial
Demettre, Edith
Mouchet, François
Djègbè, Innocent
Marin, Philippe
Chandre, Fabrice
Corbel, Vincent
Remoué, Franck
Mathieu-Daudé, Françoise
Salivary Gland Proteome Analysis Reveals Modulation of Anopheline Unique Proteins in Insensitive Acetylcholinesterase Resistant Anopheles gambiae Mosquitoes
title Salivary Gland Proteome Analysis Reveals Modulation of Anopheline Unique Proteins in Insensitive Acetylcholinesterase Resistant Anopheles gambiae Mosquitoes
title_full Salivary Gland Proteome Analysis Reveals Modulation of Anopheline Unique Proteins in Insensitive Acetylcholinesterase Resistant Anopheles gambiae Mosquitoes
title_fullStr Salivary Gland Proteome Analysis Reveals Modulation of Anopheline Unique Proteins in Insensitive Acetylcholinesterase Resistant Anopheles gambiae Mosquitoes
title_full_unstemmed Salivary Gland Proteome Analysis Reveals Modulation of Anopheline Unique Proteins in Insensitive Acetylcholinesterase Resistant Anopheles gambiae Mosquitoes
title_short Salivary Gland Proteome Analysis Reveals Modulation of Anopheline Unique Proteins in Insensitive Acetylcholinesterase Resistant Anopheles gambiae Mosquitoes
title_sort salivary gland proteome analysis reveals modulation of anopheline unique proteins in insensitive acetylcholinesterase resistant anopheles gambiae mosquitoes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125145/
https://www.ncbi.nlm.nih.gov/pubmed/25102176
http://dx.doi.org/10.1371/journal.pone.0103816
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