Cargando…

Phosphatidylcholine Alteration Identified Using MALDI Imaging MS in HBV-Infected Mouse Livers and Virus-Mediated Regeneration Defects

In this study, we investigated whether hepatitis B virus (HBV) causes the alteration of lipid metabolism and composition during acute infection and liver regeneration in a mouse model. The liver controls lipid biogenesis and bile acid homeostasis. Infection of HBV causes various liver diseases and i...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Eun-Sook, Lee, Jeong Hwa, Hong, Ji Hye, Park, Yong Kwang, Lee, Joon Won, Lee, Won-Jae, Lee, Jae Won, Kim, Kwang Pyo, Kim, Kyun-Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125171/
https://www.ncbi.nlm.nih.gov/pubmed/25101682
http://dx.doi.org/10.1371/journal.pone.0103955
_version_ 1782329738874847232
author Park, Eun-Sook
Lee, Jeong Hwa
Hong, Ji Hye
Park, Yong Kwang
Lee, Joon Won
Lee, Won-Jae
Lee, Jae Won
Kim, Kwang Pyo
Kim, Kyun-Hwan
author_facet Park, Eun-Sook
Lee, Jeong Hwa
Hong, Ji Hye
Park, Yong Kwang
Lee, Joon Won
Lee, Won-Jae
Lee, Jae Won
Kim, Kwang Pyo
Kim, Kyun-Hwan
author_sort Park, Eun-Sook
collection PubMed
description In this study, we investigated whether hepatitis B virus (HBV) causes the alteration of lipid metabolism and composition during acute infection and liver regeneration in a mouse model. The liver controls lipid biogenesis and bile acid homeostasis. Infection of HBV causes various liver diseases and impairs liver regeneration. As there are very few reports available in the literature on lipid alterations by HBV infection or HBV-mediated liver injury, we have analyzed phospholipids that have important roles in liver regeneration by using matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS) in the livers of HBV model mice. As a result, we identified different phosphatidylcholines (PCs) showing significant changes in their composition as well as cationized ion adduct formation in HBV-infected mouse livers which are associated with virus-mediated regeneration defects. To find the factor of altered PCs, the expression kinetics of enzymes was also examined that regulate PC biosynthesis during liver regeneration. It is noteworthy that the expression of choline-phosphate cytidylyltransferase A (PCYT1A) was significantly delayed in wild type HBV-expressing livers. Moreover, the amount of hepatic total PC was also significantly decreased in wt HBV-expressing mice. These results suggest that infection of HBV alters the composition of PCs which may involve in HBV-mediated regeneration defects and liver disease.
format Online
Article
Text
id pubmed-4125171
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41251712014-08-12 Phosphatidylcholine Alteration Identified Using MALDI Imaging MS in HBV-Infected Mouse Livers and Virus-Mediated Regeneration Defects Park, Eun-Sook Lee, Jeong Hwa Hong, Ji Hye Park, Yong Kwang Lee, Joon Won Lee, Won-Jae Lee, Jae Won Kim, Kwang Pyo Kim, Kyun-Hwan PLoS One Research Article In this study, we investigated whether hepatitis B virus (HBV) causes the alteration of lipid metabolism and composition during acute infection and liver regeneration in a mouse model. The liver controls lipid biogenesis and bile acid homeostasis. Infection of HBV causes various liver diseases and impairs liver regeneration. As there are very few reports available in the literature on lipid alterations by HBV infection or HBV-mediated liver injury, we have analyzed phospholipids that have important roles in liver regeneration by using matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS) in the livers of HBV model mice. As a result, we identified different phosphatidylcholines (PCs) showing significant changes in their composition as well as cationized ion adduct formation in HBV-infected mouse livers which are associated with virus-mediated regeneration defects. To find the factor of altered PCs, the expression kinetics of enzymes was also examined that regulate PC biosynthesis during liver regeneration. It is noteworthy that the expression of choline-phosphate cytidylyltransferase A (PCYT1A) was significantly delayed in wild type HBV-expressing livers. Moreover, the amount of hepatic total PC was also significantly decreased in wt HBV-expressing mice. These results suggest that infection of HBV alters the composition of PCs which may involve in HBV-mediated regeneration defects and liver disease. Public Library of Science 2014-08-07 /pmc/articles/PMC4125171/ /pubmed/25101682 http://dx.doi.org/10.1371/journal.pone.0103955 Text en © 2014 Park et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Park, Eun-Sook
Lee, Jeong Hwa
Hong, Ji Hye
Park, Yong Kwang
Lee, Joon Won
Lee, Won-Jae
Lee, Jae Won
Kim, Kwang Pyo
Kim, Kyun-Hwan
Phosphatidylcholine Alteration Identified Using MALDI Imaging MS in HBV-Infected Mouse Livers and Virus-Mediated Regeneration Defects
title Phosphatidylcholine Alteration Identified Using MALDI Imaging MS in HBV-Infected Mouse Livers and Virus-Mediated Regeneration Defects
title_full Phosphatidylcholine Alteration Identified Using MALDI Imaging MS in HBV-Infected Mouse Livers and Virus-Mediated Regeneration Defects
title_fullStr Phosphatidylcholine Alteration Identified Using MALDI Imaging MS in HBV-Infected Mouse Livers and Virus-Mediated Regeneration Defects
title_full_unstemmed Phosphatidylcholine Alteration Identified Using MALDI Imaging MS in HBV-Infected Mouse Livers and Virus-Mediated Regeneration Defects
title_short Phosphatidylcholine Alteration Identified Using MALDI Imaging MS in HBV-Infected Mouse Livers and Virus-Mediated Regeneration Defects
title_sort phosphatidylcholine alteration identified using maldi imaging ms in hbv-infected mouse livers and virus-mediated regeneration defects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125171/
https://www.ncbi.nlm.nih.gov/pubmed/25101682
http://dx.doi.org/10.1371/journal.pone.0103955
work_keys_str_mv AT parkeunsook phosphatidylcholinealterationidentifiedusingmaldiimagingmsinhbvinfectedmouseliversandvirusmediatedregenerationdefects
AT leejeonghwa phosphatidylcholinealterationidentifiedusingmaldiimagingmsinhbvinfectedmouseliversandvirusmediatedregenerationdefects
AT hongjihye phosphatidylcholinealterationidentifiedusingmaldiimagingmsinhbvinfectedmouseliversandvirusmediatedregenerationdefects
AT parkyongkwang phosphatidylcholinealterationidentifiedusingmaldiimagingmsinhbvinfectedmouseliversandvirusmediatedregenerationdefects
AT leejoonwon phosphatidylcholinealterationidentifiedusingmaldiimagingmsinhbvinfectedmouseliversandvirusmediatedregenerationdefects
AT leewonjae phosphatidylcholinealterationidentifiedusingmaldiimagingmsinhbvinfectedmouseliversandvirusmediatedregenerationdefects
AT leejaewon phosphatidylcholinealterationidentifiedusingmaldiimagingmsinhbvinfectedmouseliversandvirusmediatedregenerationdefects
AT kimkwangpyo phosphatidylcholinealterationidentifiedusingmaldiimagingmsinhbvinfectedmouseliversandvirusmediatedregenerationdefects
AT kimkyunhwan phosphatidylcholinealterationidentifiedusingmaldiimagingmsinhbvinfectedmouseliversandvirusmediatedregenerationdefects