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Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor α and Suppresses Human Breast Cancer Cell Proliferation

The Tongshu Capsule (TSC) is a prevalent form of traditional Chinese medicine widely used for its purported effects in treating mammary gland hyperplasia and inflammation. Though successful in several clinical studies, there is no clear evidence as to why TSC has a positive treatment effect, and lit...

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Detalles Bibliográficos
Autores principales: Du, Chengzhi, Li, Zhen, Wang, Shuang, Zhou, Zhongmei, Wang, Jingkun, Dong, Jian, Chen, Ceshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125192/
https://www.ncbi.nlm.nih.gov/pubmed/25101695
http://dx.doi.org/10.1371/journal.pone.0104261
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author Du, Chengzhi
Li, Zhen
Wang, Shuang
Zhou, Zhongmei
Wang, Jingkun
Dong, Jian
Chen, Ceshi
author_facet Du, Chengzhi
Li, Zhen
Wang, Shuang
Zhou, Zhongmei
Wang, Jingkun
Dong, Jian
Chen, Ceshi
author_sort Du, Chengzhi
collection PubMed
description The Tongshu Capsule (TSC) is a prevalent form of traditional Chinese medicine widely used for its purported effects in treating mammary gland hyperplasia and inflammation. Though successful in several clinical studies, there is no clear evidence as to why TSC has a positive treatment effect, and little known about underlying mechanism that may account for it. In this study, we examined the effects of TSC and found that it has a comparatively strong growth inhibition on ERα positive breast cancer cells. TSC seems to cause G1 cell cycle arrest instead of apoptosis. Interestingly, TSC also down-regulated the expression of ERα and Cyclin D1. Consistently, TSC suppressed E2 mediated ERα downstream gene expression and cell proliferation in ERα positive breast cancer cell lines MCF7 and T47D. Depletion of ERα partially abolished the effects of TSC on the decrease of Cyclin D1 and cell viability. Our findings suggest that TSC may have therapeutic effects on ERα positive breast cancers and moreover that TSC may suppress breast epithelial cell proliferation by inhibiting the estrogen pathway.
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spelling pubmed-41251922014-08-12 Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor α and Suppresses Human Breast Cancer Cell Proliferation Du, Chengzhi Li, Zhen Wang, Shuang Zhou, Zhongmei Wang, Jingkun Dong, Jian Chen, Ceshi PLoS One Research Article The Tongshu Capsule (TSC) is a prevalent form of traditional Chinese medicine widely used for its purported effects in treating mammary gland hyperplasia and inflammation. Though successful in several clinical studies, there is no clear evidence as to why TSC has a positive treatment effect, and little known about underlying mechanism that may account for it. In this study, we examined the effects of TSC and found that it has a comparatively strong growth inhibition on ERα positive breast cancer cells. TSC seems to cause G1 cell cycle arrest instead of apoptosis. Interestingly, TSC also down-regulated the expression of ERα and Cyclin D1. Consistently, TSC suppressed E2 mediated ERα downstream gene expression and cell proliferation in ERα positive breast cancer cell lines MCF7 and T47D. Depletion of ERα partially abolished the effects of TSC on the decrease of Cyclin D1 and cell viability. Our findings suggest that TSC may have therapeutic effects on ERα positive breast cancers and moreover that TSC may suppress breast epithelial cell proliferation by inhibiting the estrogen pathway. Public Library of Science 2014-08-07 /pmc/articles/PMC4125192/ /pubmed/25101695 http://dx.doi.org/10.1371/journal.pone.0104261 Text en © 2014 Du et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Du, Chengzhi
Li, Zhen
Wang, Shuang
Zhou, Zhongmei
Wang, Jingkun
Dong, Jian
Chen, Ceshi
Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor α and Suppresses Human Breast Cancer Cell Proliferation
title Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor α and Suppresses Human Breast Cancer Cell Proliferation
title_full Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor α and Suppresses Human Breast Cancer Cell Proliferation
title_fullStr Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor α and Suppresses Human Breast Cancer Cell Proliferation
title_full_unstemmed Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor α and Suppresses Human Breast Cancer Cell Proliferation
title_short Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor α and Suppresses Human Breast Cancer Cell Proliferation
title_sort tongshu capsule down-regulates the expression of estrogen receptor α and suppresses human breast cancer cell proliferation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125192/
https://www.ncbi.nlm.nih.gov/pubmed/25101695
http://dx.doi.org/10.1371/journal.pone.0104261
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