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Regional brain metabolism in a murine systemic lupus erythematosus model
Systemic lupus erythematosus (SLE) is characterized by multiorgan inflammation, neuropsychiatric disorders (NPSLE), and anti-nuclear antibodies. We previously identified a subset of anti-DNA antibodies (DNRAb) cross-reactive with the N-methyl-D-aspartate receptor, present in 30% to 40% of patients,...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126091/ https://www.ncbi.nlm.nih.gov/pubmed/24824914 http://dx.doi.org/10.1038/jcbfm.2014.85 |
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author | Vo, An Volpe, Bruce T Tang, Chris C Schiffer, Wynne K Kowal, Czeslawa Huerta, Patricio T Uluğ, Aziz M Dewey, Stephen L Eidelberg, David Diamond, Betty |
author_facet | Vo, An Volpe, Bruce T Tang, Chris C Schiffer, Wynne K Kowal, Czeslawa Huerta, Patricio T Uluğ, Aziz M Dewey, Stephen L Eidelberg, David Diamond, Betty |
author_sort | Vo, An |
collection | PubMed |
description | Systemic lupus erythematosus (SLE) is characterized by multiorgan inflammation, neuropsychiatric disorders (NPSLE), and anti-nuclear antibodies. We previously identified a subset of anti-DNA antibodies (DNRAb) cross-reactive with the N-methyl-D-aspartate receptor, present in 30% to 40% of patients, able to enhance excitatory post-synaptic potentials and trigger neuronal apoptosis. DNRAb+ mice exhibit memory impairment or altered fear response, depending on whether the antibody penetrates the hippocampus or amygdala. Here, we used 18F-fluorodeoxyglucose (FDG) microPET to plot changes in brain metabolism after regional blood–brain barrier (BBB) breach. In DNRAb+ mice, metabolism declined at the site of BBB breach in the first 2 weeks and increased over the next 2 weeks. In contrast, DNRAb− mice exhibited metabolic increases in these regions over the 4 weeks after the insult. Memory impairment was present in DNRAb+ animals with hippocampal BBB breach and altered fear conditioning in DNRAb+ mice with amygdala BBB breach. In DNRAb+ mice, we observed an inverse relationship between neuron number and regional metabolism, while a positive correlation was observed in DNRAb− mice. These findings suggest that local metabolic alterations in this model take place through different mechanisms with distinct time courses, with important implications for the interpretation of imaging data in SLE subjects. |
format | Online Article Text |
id | pubmed-4126091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-41260912014-08-14 Regional brain metabolism in a murine systemic lupus erythematosus model Vo, An Volpe, Bruce T Tang, Chris C Schiffer, Wynne K Kowal, Czeslawa Huerta, Patricio T Uluğ, Aziz M Dewey, Stephen L Eidelberg, David Diamond, Betty J Cereb Blood Flow Metab Original Article Systemic lupus erythematosus (SLE) is characterized by multiorgan inflammation, neuropsychiatric disorders (NPSLE), and anti-nuclear antibodies. We previously identified a subset of anti-DNA antibodies (DNRAb) cross-reactive with the N-methyl-D-aspartate receptor, present in 30% to 40% of patients, able to enhance excitatory post-synaptic potentials and trigger neuronal apoptosis. DNRAb+ mice exhibit memory impairment or altered fear response, depending on whether the antibody penetrates the hippocampus or amygdala. Here, we used 18F-fluorodeoxyglucose (FDG) microPET to plot changes in brain metabolism after regional blood–brain barrier (BBB) breach. In DNRAb+ mice, metabolism declined at the site of BBB breach in the first 2 weeks and increased over the next 2 weeks. In contrast, DNRAb− mice exhibited metabolic increases in these regions over the 4 weeks after the insult. Memory impairment was present in DNRAb+ animals with hippocampal BBB breach and altered fear conditioning in DNRAb+ mice with amygdala BBB breach. In DNRAb+ mice, we observed an inverse relationship between neuron number and regional metabolism, while a positive correlation was observed in DNRAb− mice. These findings suggest that local metabolic alterations in this model take place through different mechanisms with distinct time courses, with important implications for the interpretation of imaging data in SLE subjects. Nature Publishing Group 2014-08 2014-05-14 /pmc/articles/PMC4126091/ /pubmed/24824914 http://dx.doi.org/10.1038/jcbfm.2014.85 Text en Copyright © 2014 International Society for Cerebral Blood Flow & Metabolism, Inc. http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Original Article Vo, An Volpe, Bruce T Tang, Chris C Schiffer, Wynne K Kowal, Czeslawa Huerta, Patricio T Uluğ, Aziz M Dewey, Stephen L Eidelberg, David Diamond, Betty Regional brain metabolism in a murine systemic lupus erythematosus model |
title | Regional brain metabolism in a murine systemic lupus erythematosus model |
title_full | Regional brain metabolism in a murine systemic lupus erythematosus model |
title_fullStr | Regional brain metabolism in a murine systemic lupus erythematosus model |
title_full_unstemmed | Regional brain metabolism in a murine systemic lupus erythematosus model |
title_short | Regional brain metabolism in a murine systemic lupus erythematosus model |
title_sort | regional brain metabolism in a murine systemic lupus erythematosus model |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126091/ https://www.ncbi.nlm.nih.gov/pubmed/24824914 http://dx.doi.org/10.1038/jcbfm.2014.85 |
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