Cargando…

Vitamin A and Feeding Statuses Modulate the Insulin-Regulated Gene Expression in Zucker Lean and Fatty Primary Rat Hepatocytes

Unattended hepatic insulin resistance predisposes individuals to dyslipidemia, type 2 diabetes and many other metabolic complications. The mechanism of hepatic insulin resistance at the gene expression level remains unrevealed. To examine the effects of vitamin A (VA), total energy intake and feedin...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Wei, Howell, Meredith L., Li, Yang, Li, Rui, Chen, Guoxun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126667/
https://www.ncbi.nlm.nih.gov/pubmed/25105869
http://dx.doi.org/10.1371/journal.pone.0100868
_version_ 1782329943187783680
author Chen, Wei
Howell, Meredith L.
Li, Yang
Li, Rui
Chen, Guoxun
author_facet Chen, Wei
Howell, Meredith L.
Li, Yang
Li, Rui
Chen, Guoxun
author_sort Chen, Wei
collection PubMed
description Unattended hepatic insulin resistance predisposes individuals to dyslipidemia, type 2 diabetes and many other metabolic complications. The mechanism of hepatic insulin resistance at the gene expression level remains unrevealed. To examine the effects of vitamin A (VA), total energy intake and feeding conditions on the insulin-regulated gene expression in primary hepatocytes of Zucker lean (ZL) and fatty (ZF) rats, we analyze the expression levels of hepatic model genes in response to the treatments of insulin and retinoic acid (RA). We report that the insulin- and RA-regulated glucokinase, sterol regulatory element-binding protein-1c and cytosolic form of phosphoenolpyruvate carboxykinase expressions are impaired in hepatocytes of ZF rats fed chow or a VA sufficient (VAS) diet ad libitum. The impairments are partially corrected when ZF rats are fed a VA deficient (VAD) diet ad libitum or pair-fed a VAS diet to the intake of their VAD counterparts in non-fasting conditions. Interestingly in the pair-fed ZL and ZF rats, transient overeating on the last day of pair-feeding regimen changes the expression levels of some VA catabolic genes, and impairs the insulin- and RA-regulated gene expression in hepatocytes. These results demonstrate that VA and feeding statuses modulate the hepatic insulin sensitivity at the gene expression level.
format Online
Article
Text
id pubmed-4126667
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41266672014-08-12 Vitamin A and Feeding Statuses Modulate the Insulin-Regulated Gene Expression in Zucker Lean and Fatty Primary Rat Hepatocytes Chen, Wei Howell, Meredith L. Li, Yang Li, Rui Chen, Guoxun PLoS One Research Article Unattended hepatic insulin resistance predisposes individuals to dyslipidemia, type 2 diabetes and many other metabolic complications. The mechanism of hepatic insulin resistance at the gene expression level remains unrevealed. To examine the effects of vitamin A (VA), total energy intake and feeding conditions on the insulin-regulated gene expression in primary hepatocytes of Zucker lean (ZL) and fatty (ZF) rats, we analyze the expression levels of hepatic model genes in response to the treatments of insulin and retinoic acid (RA). We report that the insulin- and RA-regulated glucokinase, sterol regulatory element-binding protein-1c and cytosolic form of phosphoenolpyruvate carboxykinase expressions are impaired in hepatocytes of ZF rats fed chow or a VA sufficient (VAS) diet ad libitum. The impairments are partially corrected when ZF rats are fed a VA deficient (VAD) diet ad libitum or pair-fed a VAS diet to the intake of their VAD counterparts in non-fasting conditions. Interestingly in the pair-fed ZL and ZF rats, transient overeating on the last day of pair-feeding regimen changes the expression levels of some VA catabolic genes, and impairs the insulin- and RA-regulated gene expression in hepatocytes. These results demonstrate that VA and feeding statuses modulate the hepatic insulin sensitivity at the gene expression level. Public Library of Science 2014-08-08 /pmc/articles/PMC4126667/ /pubmed/25105869 http://dx.doi.org/10.1371/journal.pone.0100868 Text en © 2014 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Wei
Howell, Meredith L.
Li, Yang
Li, Rui
Chen, Guoxun
Vitamin A and Feeding Statuses Modulate the Insulin-Regulated Gene Expression in Zucker Lean and Fatty Primary Rat Hepatocytes
title Vitamin A and Feeding Statuses Modulate the Insulin-Regulated Gene Expression in Zucker Lean and Fatty Primary Rat Hepatocytes
title_full Vitamin A and Feeding Statuses Modulate the Insulin-Regulated Gene Expression in Zucker Lean and Fatty Primary Rat Hepatocytes
title_fullStr Vitamin A and Feeding Statuses Modulate the Insulin-Regulated Gene Expression in Zucker Lean and Fatty Primary Rat Hepatocytes
title_full_unstemmed Vitamin A and Feeding Statuses Modulate the Insulin-Regulated Gene Expression in Zucker Lean and Fatty Primary Rat Hepatocytes
title_short Vitamin A and Feeding Statuses Modulate the Insulin-Regulated Gene Expression in Zucker Lean and Fatty Primary Rat Hepatocytes
title_sort vitamin a and feeding statuses modulate the insulin-regulated gene expression in zucker lean and fatty primary rat hepatocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126667/
https://www.ncbi.nlm.nih.gov/pubmed/25105869
http://dx.doi.org/10.1371/journal.pone.0100868
work_keys_str_mv AT chenwei vitaminaandfeedingstatusesmodulatetheinsulinregulatedgeneexpressioninzuckerleanandfattyprimaryrathepatocytes
AT howellmeredithl vitaminaandfeedingstatusesmodulatetheinsulinregulatedgeneexpressioninzuckerleanandfattyprimaryrathepatocytes
AT liyang vitaminaandfeedingstatusesmodulatetheinsulinregulatedgeneexpressioninzuckerleanandfattyprimaryrathepatocytes
AT lirui vitaminaandfeedingstatusesmodulatetheinsulinregulatedgeneexpressioninzuckerleanandfattyprimaryrathepatocytes
AT chenguoxun vitaminaandfeedingstatusesmodulatetheinsulinregulatedgeneexpressioninzuckerleanandfattyprimaryrathepatocytes